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Short-term toxicity to fish

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Description of key information

LC50 (96h) > 100 mg/L (nominal) for Danio rerio (OECD 203)
measured concentrations after 96 h: 5.64 mg Mo/L, 0.14 mg Al/L

Key value for chemical safety assessment

Additional information

Two studies were conducted with the test item aluminium molybdenum oxide. In a GLP guideline study by Muckle (2013) conducted according to OECD 203, Danio rerio were exposed to a nominal concentration of 100 mg/L in a static limit test. One day before the start of the test, a filtered test solution was prepared (24 h shaking, 0.45 µm filter). At the beginning and at the end of the test, the content of molybdenum and aluminium as components of the test item (10.1% Al and 53.9% Mo) was determined using a validated AAS-method. After 96 h, 5.64 mg Mo/L and 0.14 mg Al/L was measured. The obtained measured concentrations show that the test item was present in the solution and sufficient dissolution was achieved. The recoveries after 96 hours were 98% for Mo and 117% for Al of the start concentration. No mortalities or any abnormal behaviour were observed. Thus, the 96h-LC50 and NOEC were determined to be >100 mg/L and 100 mg/L, respectively, based on nominal concentrations.

This result is supported in a non-GLP study by Chen (2013) conducted according to a national guideline similar to OECD guideline 203. The test species was Gobiocyprus rarus, otherwise the test procedure was the same as in the study by Muckle (2013). Also in this study recoveries showed that the test item was present in the solution and sufficient dissolution was achieved. After 96 h, 13.5 mg Mo/L and 0.0394 mg Al/L was measured. No mortalities or any abnormal behaviour was observed. Consequently, the 96h-LC50 and NOEC are reported to be >100 mg/L and 100 mg/L, respectively.