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EC number: 230-224-6 | CAS number: 6974-77-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The acute oral toxicity of 1 -bromo-3 -chloro-2 -methylpropane was invesigated in four groups of five male and five female CD rats.
Under this test conditions the acute oral median lethal dosage (LD50) of 1 -bromo-3 -chloro-2 -methylpropane was 2248 mg/kg for males, 2239 mg/kg for females and 2243 mg/kg combined.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-2-10 to 1995-3-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- 1987 version
- Qualifier:
- according to guideline
- Guideline:
- other: US EPA guideline (1985)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Specific details on test material used for the study:
- Batch Number 48241
- Species:
- rat
- Strain:
- other: CD strain
- Remarks:
- remote Sprague-Dawley origin
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Limited (UK)
- Females (if applicable) nulliparous and non-pregnant: [yes/no] not specified
- Age at study initiation: approximately five weeks
- Weight at study initiation: for males from 111 - 163 g for females from 103 - 172 g
- Fasting period before study: Overnight
- Housing:five animals of the same sex are housed in stainless grid cages (RS Biotech, Northants, England)
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum):ad libitum
- Acclimation period: at least five days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): Target ; 21 C (19 - 25 C)
- Humidity (%): Target 55 % R.H.(40 % - 70% RH)
- Air changes (per hr): at least 10 complete air changes per hour without re-circulating
- Photoperiod (hrs dark / hrs light): 12 hrs darks/12 hrs light
IN-LIFE DATES: From: 1 To:15 days - Route of administration:
- oral: gavage
- Vehicle:
- maize oil
- Details on oral exposure:
- The test material was prepared at appropriate concentrations in maize oil to permit administration at constant volume-dosage of 10 ml/kg bodyweight
- Doses:
- 2000, 2515, 3162 and 5000 mg/kg bodyweight
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- Observation: Three separate recordings of signs were made during the first hour after dosing and two further recordings during the remainder of Day1. From Day 2 onwards, the animals were inspected twice daily and recordings were made one daily. Circumstances of any death were recorded. ANy animal showing severe or enduring signs of pain or distress was humanely killed. The bodyweight of each animal was recorded on the day before dosing and on Days 1, 8 and 15. The test was terminated the morning of Day 15.
Necropsy: Carcases were stored in a refrigerator at approximately 4 C until traiined necropsy staff were available. Surviving animals were killed by carbon dioxide inhalation at termination of the study.
All animals were throughly examined for abnormality of tissues or organs. All body cavities were opened, larger organs were sectioned and the gastro-intestinal tract was opened at intervals for examination of the mucosal surfaces. All abnormalities were described or the normal appearance of majo organs was confirmed.
Macroscopically abnormal tissues were preserved in 4% neutral buffered formaldehyde and retained against future possible histopathological requirement. - Statistics:
- Probit analysis by the method of Finney (1971) was used to determine the acute median lethal dosage, 95% confidence interval and slope of the dose response curve of the test material for both sexes. The calculation were performed by the GLIM statistics program (Baker and Neder, 1978) using a special macro program developed by Baker (Baker, 1980)
- Preliminary study:
- The preliminary study was carried out using two groups of one male and one female rat given an oral administration of 2-MBCP at dosage of 800 or 2000 mg/ke, at a constant volume-dosage of 10 ml/kg in maize oil. There were no deaths, but significant signs were obsered at 2000 mg/kg.
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 239 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 981 - <= 2 497
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2 248 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 714 - <= 2 781
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 22 434 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 2 093 - <= 2 393
- Remarks on result:
- other: combined
- Mortality:
- The majority of animals treated at 2515 mg/kg or above died. The deaths occurred during the first four days after dosing. There was a single death, on Day 3, at the low dosage of 2000 mg/kg.
- Clinical signs:
- Ante mortem signs comprised lethargy, under activity, prone position, nconsciousness, reddening, staggering gait, tremor, slow deep respiration, gasping, piloerection, ungroomed fur, hypothermia, pigmented staining of the snout, ocular discharge, hunched posture and closed eyes. Four animals treated at 3162 mg/kg appeared to show some stereotype behaviour (continous ear scratching).
Signs of reaction in animals surviving to termination comprised under activity, prone position, reddening staggering gait, lethargy, respiratory abnormalities, ungroomed fur, hunched posture, thin body conformation and closed eyes.
Suvivors treated at 2000 mg/kg were overtly normal to Day 5, while the suvivor treated at 2515 mg/kg had fully recovered by Day 10. - Body weight:
- The surviving animals achieved aniticipated bodyweigjht gains.
- Gross pathology:
- Necropsy findings for the decendents comprised incidences of altered gastro-intestinal contents, distended urinary bladder, firm luinmgs and fur staining. Necropsy of the survivors, on Day 15, revealed no significant lesion.
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Under the conditions of this study the acute oral median lethal dosage (LD50) of 1-bromo-3-chloro-2-methylpropane was 2248 mg/kg (1714 - 2781 mg/kg) for males, 2239 mg/kg (1981 - 2947 mg/kg) for females and 2243 mg/kg (2093 - 2393 mg/kg) combined.
- Executive summary:
Under the conditions of this study the acute oral median lethal dosage (LD50) of 1-bromo-3-chloro-2-methylpropane was 2248 mg/kg for males, 2239 mg/kg for females and 2243 mg/kg combined. This substance was classified as category 4 based on this study.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 243 mg/kg bw
Additional information
Justification for classification or non-classification
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