N-(butoxymethyl)acrylamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

See the Analogue Approach Report attached in Section 13 of the IUCLID dossier.

Reason / purpose for cross-reference:

other: Read across to target substance

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Diluent: US EPA moderately hard reconstituted
Solvent: None required
Controls: Diluent only

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Strain: Onchorhyncus mykiss
- Source: Aquatic Research organisms, Hampton, NH
- Age at study initiation: 64 days (Hatch: 11/09/90)
- Length at study initiation: ≤50 mm total length, uniform size
- Feeding during test: None
ACCLIMATION:Test fish were acclimated to the receiving water for a minimum of 24 hours

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

80-100 mg/L as CaC03.

Test temperature:

15°C±2°C

pH:

7.4 to 7.8.

Dissolved oxygen:

7.4-8.6

Nominal and measured concentrations:

Nominal : 0, 62.5, 125, 250, 500, 1000 ppm

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 4L polypropylene vessels; exposure volume 3000 ml
- Aeration: free air supplied at ≤100 bubbles per minute to maintain ≥ 60% saturation.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per vehicle control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: EPA moderately hard reconstituted (from EPA 600/4-85/013):
Reconstituted freshwater
NaHCO3 = 96 mg/L
CaSO4.2H20 = 60 mg/L
MgSO4 = 60 mg/L
KCI = 4 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16hr/8hr light/dark cycle
- Light intensity: fluorescent, 50 to 100 foot-candles (lab ambient)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, behaviour (lethargy, erratic swimming), and physiology (discoloration), initially, and daily thereafter.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

500 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not obtainable graphical interpolation

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

360 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not obtainable graphical interpolation

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

240 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not obtainable graphical interpolation

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

180 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not obtainable graphical interpolation

Sublethal observations / clinical signs:

Acute Toxicity versus Onchorhyncus mykiss Definitive Test - CT 444 90 D

Conc.( ppm)	Number Organism	Cumulative Mortality
		24 h	48 h	72 h	96 h	% Mortality
0 control	10	0	0	0	0	0
62.5	10	0	0	0	1	10
125	10	0	0	0	0	0
250	10	0	0	6	10	100
500	10	5	10	10	10	100
1000	10	10	10	10	10	100

Validity criteria fulfilled:

yes

Remarks:

all of control organisms survived 96 hours

Conclusions:

Acute toxicity of acrylamide (CT 444 90 D) to the Rainbow Trout, Onchorhyncus mykiss, was determined to be 180 ppm.

Description of key information

The acute toxicity of the read-across material, acrylamide, to fish was assessed according to OECD Test Guideline 203. The 96 h LC50 was 180 ppm.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

180 mg/L

Additional information

The short term toxicity to fish of the read-across material, acrylamide, was assessed according to OECD Test Guideline 203. Freshwater trout (Oncorhynchus mykiss) were exposed to the read-across material for 96 hours in a static freshwater test at nominal concentrations of 0, 62.5, 125, 250, 500 and 1000 ppm in open 4 L polypropylene vessels.  Mortality, behaviour (lethargy, erratic swimming), and physiology (discoloration) were measured initially and daily thereafter. 

At 250, 500 and 1000 ppm there was 100 % mortality. No mortality was observed at 125 ppm, and 10 % mortality at 62.5 ppm. The LC50 value was based on graphical interpolation.

The 96 hour LC50 was 180 ppm.