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EC number: 216-629-0 | CAS number: 1630-78-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 May 2020 - 30 July 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- TEST MATERIAL
- Source and lot/batch number of test material: N1200115
- Purity: 99.9989%
- Storage: gas cylinder
- Appearance: colourless gas - Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Secondary effluent of a sewage treatment plant treating predominantly domestic sewage in Kurame-shi, Fukuoka, Japan.
- Sampling date: 27 May 2020 - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 7.62 mg/L
- Details on study design:
- Preparation of test
a) Preparation of inoculum: the collected secondary effluent was filtered through a No. 2 filter paper and the filtrate was used as inoculum. The inoculum was kept under aerobic conditions until used.
b) Preparation of mineral medium: The mineral medium (6L) was prepared: each 1 mL of stock solutions (a), (b), (c) and (d) prescribed in the test guideline were made up to 1 L with purified water. After the preparation, it was aerated for 20 min and stood still for 24.0 hours at a test temperature.
c) Addition of inoculum: The prepared inoculum (a) was added into the mineral medium at the rate of 0.05 mL of the inoculum per litre of the mineral medium.
Preparation of test solutions
The following test solutions were prepared by using 3 test bottles and incubated.
a) Abiotic control: test solutions (water + teste item, n=2): each test bottle with purified water and 200 µL of the test item aqueous solution (0.762 mg as the test item) was added into it with a microsyringe, giving he concentration of the test item of 7.62 mg/L.
b) Test suspension: test solution (inoculum + test item, n=12): each test bottle was filled with inoculated mineral medium and 200 µL of the test item aqueous solution (0.762 mg as the test item) was added into it with a microsyringe, giving final test item concertation of 7.62 mg/L.
c) Control: Test solution (inoculum + sodium benzoate, n=10): Each test bottle was filled with 3.00 mg/L was prepared with the inoculated mineral medium.
d) Blank inoculum (n=11): Each bottle was filled with inoculated mineral medium.
Conditions of incubation:
Test vessel: glass bottle (100 mL)
Temperature: 20±1° (measured value: 19.3 – 20.6 °C)
Incubation duration: 28 days (under dark condition)
Observation: during the incubation period, appearances of the test solutions were observe once a day. The incubation temperature was measured and recorded once a day. - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The validity criteria of the test were met.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- BOD
- Value:
- ca. -1
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Remarks:
- by GC
- Value:
- ca. 0
- Sampling time:
- 28 d
- Results with reference substance:
- Percentage biodegradation of sodium benzoate by BOD on Day 14: 71%, 70%
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item was not biodegradable under the test conditions of this study.
- Executive summary:
Summary:
Objetive: This study was aimed at evulationg the biodegradability of TKN1 by microorganisms.
Test Guideline: OECD No 301D "Ready Biodegradability: Close Bottle Test
Conditions of incubation:
Concentration of test item: 7.62 mg/L
Inoculum: Filtrate of secondary effluent of a sewage treatment plant treating predominatly domestic sewage.
Concentration of inoculum: 0.05 mL/L
Volume of test solution: 100 mL
Incubation temperature: 20°C
Incubation condition: Stationary incubation in sealed bottles.
Incubation duration: 28 days (under dark condition)
Measurement and analysis for calculation of percentage biodegradation:
a) Measurement of disssolved oxygen (DO) by diaphragm electrode mehtod.
b) Determination of test item by gas chromatography (GC).
Results:
Percentage biodegradation by biochemical oxygen demand (BOD) at Day 28: average -1%
Percentage biodegradation of test item (by GC) at Day 28: average 0%
Conclusion:
The test item was not biodegraded under the test conditions of this study.
Reference
Analytical results of the test solutions on Day 28:
|
Water + test item |
Inoculum + test item |
Theoretical amount |
|||
BOD |
mg O2/L |
- |
- |
-0.05 |
-0.05 |
7.62 |
Residual amount and percentage residue of test item (by GC) |
mg |
0.761 |
0.746 |
0.756 |
0.754 |
0.762 |
% |
100 |
98 |
99 |
99 |
- |
Percentage biodegradation:
|
Inoculum + test item [mean value] |
||||
Day 7 |
Day 14 |
Day 21 |
Day 28 |
||
Percentage biodegradation by BOD |
% |
2 2 [2] |
1 2 [1] |
0 1 [0] |
-1 -1 [-1] |
Percentage biodegradation of test item (by GC) |
% |
- - - |
- - - |
- - - |
0 0 [0] |
Averages of the percentage biodegradation on Day 28 by BOD and GC were -1% and 0%, respectively. Moreover, no peak corresponding to a degradation product was detected on the GC chromatograms. The results showed that the test item was not biodegraded under the test conditions of this study.
Validity of test condition
The test was valid:
|
|
Value in this test |
Value of criterion |
Difference between extremes of replicate values of percentage biodegradation on test solutions (inoculum + test item) on Day 28 |
BOD |
0% |
< 20 % |
Test item (by GC) |
0% |
||
Percentage biodegradation of sodium benzoate by BOD on Day 14 |
71%, 70% |
> 60 % |
|
Amount of oxygen depletion in test solution (inoculum blank) on Day 28 |
0.56 mg O2/L |
≤ 1.5mg O2/L |
|
Residual concentration of oxygen in each bottle during the incubation period |
≥5.04 mg O2/L |
≥0.5 mg O2/L |
Description of key information
The ready biodegradability of TKN1 was assessed (CERI, 2020, Study 16698). The procedure was designed to be compatible the OECD test guidelines 301D, and was conducted in compliance with GLP.
The parameter determined in the test was the percentage biodegradation by biochemical oxygen demand after 28 days of incubation at temperatures nominally in the range 20 ± 1°C. TNK1 was added to bottles containing mineral salts medium inoculated with activated sludge at a nominal test concentration of 7.62 mgO2/L. Control cultures contained inoculated mineral salts medium alone and inoculated mineral salts medium plus the reference substance sodium benzoate (3.0 mgO2/L).
Averages of the percentage biodegradation on Day 28 by BOD and GC were -1% and 0%, respectively. Moreover, no peak corresponding to a degradation product was detected on the GC chromatograms. The results showed that the test item was not biodegraded under the test conditions of this study.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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