Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 211-322-8 | CAS number: 638-16-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-04-14 to 2006-04-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: "Method concerning tests relating to new chemical substances"
- Version / remarks:
- No.1121002 by the Pharmaceutical and Food Safety Bureau, 2003; No.2 by the Manufacturing Bureau directive, 2003; No. 021121002 Environmental Protection Agency Planning Directive
- Principles of method if other than guideline:
- method similar to OECD203 performed under GLP; with analytical verification of test substance
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- EC Number:
- 211-322-8
- EC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- Cas Number:
- 638-16-4
- Molecular formula:
- C3H3N3S3
- IUPAC Name:
- 1,3,5-triazine-2,4,6(1H,3H,5H)-trithione
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The freshly prepared test solutions at test start and water renewal and the old solutions at water renewal were sampled for analysis.
- Sampling method: The analytical samples were collected 10 mL each with graduation of the test tube in the glass-stoppered 10mL capacity test tubes from the middle layer of the test liquid in the test container using 10 mL capacity Komagome pipettes.
- Sample storage conditions before analysis: Samples were stored at 4 °C until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 500 mg of test substance were added to dilution water and the mixture was placed in an ultrasonic bath at 24 °C for 10 min. Afterwards, the volume was adjusted to 5 L.
Test organisms
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Source: In-house rearing within the institute of environmental ecology
- Age at study initiation (mean and range, SD): 6 months
- Length at study initiation (length definition, mean, range and SD): 2.9 cm (2.7 to 3.0 cm)
- Weight at study initiation (mean and range, SD): 0.23 g (0.20 to 0.26 g)
- Feeding during test: None
ACCLIMATION
- Acclimation period: 7 days in a flow water system, 24 h at the same conditions as the test
- Type and amount of food: Tetramine, ca. 2% of fish bodyweight/day
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- prolongation to 120 h
Test conditions
- Hardness:
- 59 mg/L as CaCO3
- Test temperature:
- 23.7 - 24.1° C (control group), 23.5 - 24.8° C (concentration groups)
- pH:
- 7.5 - 7.9 (control group), 6.1 - 6.8 (concentration groups)
- Dissolved oxygen:
- 7.0 - 8.5 mg/L (control group), 7.1 - 8.8 mg/L (concentration groups)
- Nominal and measured concentrations:
- Nominal: control, 100 mg/L
Measured: 102.4 mg/L (geometric mean) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Container
- Type (delete if not applicable): covered with glass
- Material, size, fill volume: Glass, 12 L, 5 L
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): Test water was renewed every 24 h.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Intervals of water quality measurement: Temperature, oxygen and pH were measured every 24 h.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
- Light intensity: White fluorescent lamp
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Abnormalities or mortalities were recorded after 3, 6, 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No mortality was observed. Therefore, a limit test with a test substance concentration of 100 mg/L has been conducted in the main study. - Reference substance (positive control):
- yes
- Remarks:
- copper sulfate (II) pentahydrate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 102.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 120 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 102.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No toxicity symptoms were observed during th exposure duration period (120 h).
- Mortality of control: None
- Other adverse effects control: None
- Analytics: The measured test substance concentration in the freshly prepared media ranged from 100.8 to 103.9% of nominal values. In the old media, the measured concentration was 102.2 to 102.8% of nominal values. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- LC50 (96h): 0.26 mg/L (95% CL: 0.18 - 0.37 mg/L)
- Date of experiment: 26 Nov - 30 Nov 2005
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 for the test substance was > 102.4 mg/L after 96 h under the conditions reported.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.