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EC number: 230-262-3 | CAS number: 6994-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1986-1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Principles of method if other than guideline:
- Similar to OECD 423.
- GLP compliance:
- no
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Ethyl 3-amino-1H-pyrazole-4-carboxylate
- EC Number:
- 230-262-3
- EC Name:
- Ethyl 3-amino-1H-pyrazole-4-carboxylate
- Cas Number:
- 6994-25-8
- Molecular formula:
- C6H9N3O2
- IUPAC Name:
- ethyl 3-amino-1H-pyrazole-4-carboxylate
- Details on test material:
- - Name of test material (as cited in study report): Karbetoxi-pirazol
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance):
- Smiles notation (if other than submission substance):
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: white or yellowish-white crystalline powder
- Analytical purity:
- Impurities (identity and concentrations):
- Composition of test material, percentage of components:
- Isomers composition:
- Purity test date:
- Lot/batch No.:
- Expiration date of the lot/batch:
- Radiochemical purity (if radiolabelling):
- Specific activity (if radiolabelling):
- Locations of the label (if radiolabelling):
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions: stable
- Storage condition of test material: room temperature
- Other:
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source:
- Age at study initiation:
- Weight at study initiation: 193.00 +/-15.3 g (male), 153.00 +/- 6.3 g (female)
- Fasting period before study: 18 h
- Housing:
- Diet (e.g. ad libitum): LATI food
- Water (e.g. ad libitum): ad libitum
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 1.5
- Humidity (%):
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12 h/12 h
IN-LIFE DATES: From: To:
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 1 % methylcellulose in water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 1 % methylcellulose
- Amount of vehicle (if gavage):
- Justification for choice of vehicle:
- Lot/batch no. (if required):
- Purity:
MAXIMUM DOSE VOLUME APPLIED: 5000 mg/kg
DOSAGE PREPARATION (if unusual):
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose:
Dose selection according to Deichmann-Le Blanck.
5 doses, 3-3 animals/dose. - Doses:
- 360 mg/kg
510 mg/kg
552 mg/kg
600 mg/kg
620 mg/kg
710 mg/kg
840 mg/kg
1000 mg/kg - No. of animals per sex per dose:
- Dose selection (approximate LD50 test)
360 mg/kg 2 male/2 female
510 mg/kg 2 male/2 female
710 mg/kg 2 male/2 female
1400 mg/kg 2 male/2 female
2600 mg/kg 2 male/2 female
360 mg/kg 6 male/6 female
510 mg/kg 6 male/6 female
552 mg/kg 6 male/6 female
600 mg/kg 6 male/6 female
620 mg/kg 6 male/6 female
710 mg/kg 6 male/6 female
840 mg/kg 6 male/6 female
1000 mg/kg 6 male/6 female - Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 2 h, 4h, 24h, 48 h, 72 h and 7. day, 14. day
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, relative organ weights, histopathology
Results and discussion
- Preliminary study:
- Dose Male Female
(mg/kg) (dead/alive) (dead/alive)
360 ( -/2) symptom free survivors (-/2) survivors with symptoms
510 (- /2) survivors with symptoms (2/2) dead in 4 hours
710 (- /2) survivors with symptoms (2/2) dead in 4 hours
1400 (2/2) dead in 2 hours (2/2) dead in 1/2 hours
2600 (2/2) dead in 1/2 hours (2/2) dead in 10 min.
Females were more sensitive, they exited at 510 mg/kg doses.
Males were exited at 2600 mg/kg doses.
Approximative lethal dose is 2600 mg/kg.
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- approximate LD50
- Effect level:
- 2 600 mg/kg bw
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 673.674 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 611.44 - < 747.17
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 573.27 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 529.27 - < 620.14
- Mortality:
- Males
Doses Dead/Alive Time
360 mg/kg 0/6 --
510 mg/kg 1/6 in 4 hours
552 mg/kg 1/6 in 4 hours
600 mg/kg 1/6 between 4-48 hours
620 mg/kg 3/6 in 4 hours
710 mg/kg 3/6 2-72 hours
840 mg/kg 5/6 in 4 hours
1000 mg/kg 6/6 in 1/2 hours - Clinical signs:
- other: Disorientation. Cramps, rolling after 10-15 minutes time. Cyanosis, tachicardia, death. Survivors hair were ruffled. No food consumptions because lack of appetite in the first 2 days. After the 3rd-4th day the body weight gain became normal.
- Gross pathology:
- Clinical signs and pathology has shown nerve system effects.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The substance is harmful if swallowed according to EU classifications.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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