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EC number: 221-111-2 | CAS number: 3006-86-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-02-15 to 2000-02-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 4 April 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Each test solution was prepared by direct addition of the test substance in the test flask with an appropriate micropipette immediately before the start of the test.
- Controls: yes (two controls) - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: no
- Source: the water treatment plant Emeraude (SIARR); 76141 Petit-Quevilly, France
- Preparation of inoculum for exposure: the inoculum was left to settle and the supernatant rejected. It was then filtered and washed with dechlorinated water. Four samples of 50 mL were taken to determine the content in suspended matter (dry weight). The concentration of sludge (8.80 g/L) was then adjusted to 4 g/L by addition of the appropriate volume of dechlorinated water.
- Initial biomass concentration: 1.6 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 280 +/- 20 mg/L as CaCO3
- Test temperature:
- 22 °C
- Nominal and measured concentrations:
- - nominal concentration: 2, 10 and 20 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks which were made up to 300 mL with water
- Type: open
- Aeration: air was bubbled through each solutions at the rate of 0.5 to 1 L/min until the start of oxygen concentration measurements
- No. of organisms per vessel: 200 mL of inoculum
- No. of vessels per concentration: 2
- No. of vessels per control: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5- Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The respiration rate of the test item solution at the highest concentration was equivalent to the respiration rate of the first control. Therefore, the oxygen consumption rate of the two other test solutions was not determined.
Based on the inhibition percentage of the respiration rate obtained in the test solution of highest concentration, the 3-hour EC10 of the test substance was also > 20 mg/L. - Results with reference substance (positive control):
- The EC50 (3 h) of 3,5-Dichlorophenol was 14 mg/L (95 % confidence limits 12 - 17 mg/L).
- Reported statistics and error estimates:
- Determination of the EC50:
When for at least two concentrations, inhibition is > 0% and < 100 %, the EC50 is calculated according to Probit analysis. The confidence interval limits are calculated statistically according to Fieller`s method.
When at only one concentration, inhibition is > 0 % and < 100 %, the EC50 is also calculated by Probit analysis. In this case, the highest concentration causing no inhibition and lowest concentration producing 100 % inhibition are used as confidence limits.
If at all concentrations inhibition is 0 or 100 %, the EC50 corresponds to the geometric mean of the highest concentration causing no inhibition and the lowest concentration producing 100 % inhibition. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour EC50 of cyclohexylidenebis[tert-butyl]peroxide was determined to be > 20 mg/L for activated sludge. The 3-hour EC10 of the test substance was > 20 mg/L.
- Executive summary:
The acute toxicity of cyclohexylidenebis[tert-butyl]peroxide on micro-organismus present in activated sludge was evaluated using a 3-hour static test according to OECD guideline no. 209. Three test solutions (2, 10 and 20 mg/L) were tested. The respiration rate of the test solution at the highest concentration (20 mg/L) was equivalent to the respiration rate of the first control. Therefore, the oxygen consumption rate of the two other test solutions was not determined. The 3-hour EC10 and the 3-hour EC50 of the test substance cyclohexylidenebis[tert-butyl]peroxide were determined to be > 20 mg/L.
Reference
Description of key information
The 3-hour EC50 of cyclohexylidenebis[tert-butyl]peroxide was determined to be > 20 mg/L for activated sludge. The 3-hour EC10 of the test substance was > 20 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 20 mg/L
Additional information
The acute toxicity of cyclohexylidenebis[tert-butyl]peroxide on micro-organismus present in activated sludge was evaluated using a 3-hour static test according to OECD guideline no. 209. Three test solutions (2, 10 and 20 mg/L) were tested. The respiration rate of the test solution at the highest concentration (20 mg/L) was equivalent to the respiration rate of the first control. Therefore, the oxygen consumption rate of the two other test solutions was not determined. The 3-hour EC10 and the 3-hour EC50 of the test substance cyclohexylidenebis[tert-butyl]peroxide were determined to be > 20 mg/L.
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