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EC number: 240-464-3 | CAS number: 16415-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Remarks:
- prolonged to 60 d
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Jul - 24 Sep 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt Hildesheim
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, Hildesheim, Germany
- Pretreatment: Sludge was washed twice with chlorine free tap water. After the second washing settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration with CO2 free air until test start (2 d). The amount of inoculum used to initiate inoculation was 3.83 mg/L (25 mg/L dw)
- Initial cell/biomass concentration: Colony Forming Units (CFU) of the inoculum were determined prior to test start by standard dilution plate count. The CFU concentration of the inoculum corresponds to approx. 0.52 x 10^7 CFU/L in the final test solution.
- Water filtered: no - Duration of test (contact time):
- 60 d
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Initial conc.:
- 51.2 other: mg O2/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Solubilising agent: silicone oil (500 µL in 250 mL test solution, 100 mg test item / 10 mL silicone oil)
- Test temperature: 21 - 21.7 °C
- pH: inoculum control: 7.51 - 7.65, functional control: 7.64 - 7.68, inoculum control with silicone oil: 7.51 - 7.73, test item: 7.5 - 7.71, toxicity control: 7.64 - 7.73
- pH adjusted: no
- Continuous darkness: yes
- Other: continuous stirring
TEST SYSTEM
- Culturing apparatus: 500 mL brown glass bottles filled with 250 mL
- Number of culture flasks/concentration: 2
- Measuring equipment: OxiTop measuring heads
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: A rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2.
SAMPLING
- Sampling frequency and method: The oxygen consumption was determined in the incubation vessels by the OxiTop measuring system at 360 measuring points (every 240 min) during the 60 d incubation period.
CONTROL AND BLANK SYSTEM
- Inoculum control: 2 replicates (test medium without test and / or reference item)
- Inoculum control with silicone oil: 2 replicates (test medium without test and / or reference item and 500 µL silicone oil)
- Toxicity control: 1 replica (test item (incl. 500 µL silicone oil) and reference item)
- Functional control: 1 replica
- Functional control with silicone oil: 1 replica - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 30 mg/L
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 30 mg/L sodium benzoate and 500 µL silicone oil / 250 mL test solution
- Parameter:
- % degradation (O2 consumption)
- Value:
- 65
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 81
- Sampling time:
- 60 d
- Details on results:
- - The biodegradation of the substance failed the 10 - d window criterion.
- O2 depletion in inoculum control with silicon oil: 28.6 mg O2/L on Day 28 and 33.5 mg/L on Day 60.
- Toxicity control: 47% degradation of test item after 14 d and 86% after 60 d. - Results with reference substance:
- - Functional control: 86% biodegradation of sodium benzoate on Day 14.
- Functional control with silicone oil: 98% biodegradation on Day 60. - Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Interpretation of results:
- readily biodegradable, but failing 10-day window
Reference
Table 1: % Biodegradation of test item, functional control and toxicity control during 60 d.
[d] |
% Biodegradation |
||||
Functional Control |
Functional control with silicon oil |
Test Item 1 |
Test Item 2 |
Tox. Control |
|
2 |
30 |
30 |
3 |
1 |
14 |
4 |
64 |
66 |
3 |
3 |
33 |
6 |
75 |
78 |
3 |
3 |
39 |
8 |
83 |
86 |
4 |
4 |
43 |
10 |
84 |
90 |
9 |
9 |
45 |
12 |
85 |
90 |
23 |
20 |
46 |
14 |
86 |
90 |
36 |
34 |
47 |
16 |
81 |
90 |
40 |
41 |
48 |
18 |
78 |
93 |
46 |
45 |
55 |
20 |
77 |
94 |
51 |
52 |
62 |
22 |
78 |
94 |
56 |
57 |
67 |
24 |
78 |
93 |
59 |
60 |
70 |
26 |
78 |
94 |
62 |
64 |
72 |
28 |
79 |
94 |
64 |
66 |
74 |
30 |
80 |
95 |
65 |
68 |
76 |
32 |
80 |
94 |
67 |
70 |
78 |
34 |
80 |
96 |
67 |
72 |
79 |
36 |
79 |
96 |
70 |
74 |
80 |
38 |
77 |
97 |
70 |
74 |
80 |
40 |
80 |
95 |
71 |
76 |
80 |
42 |
81 |
94 |
72 |
78 |
81 |
44 |
80 |
96 |
73 |
79 |
82 |
46 |
82 |
97 |
75 |
79 |
83 |
48 |
82 |
96 |
75 |
80 |
83 |
50 |
81 |
96 |
75 |
80 |
83 |
52 |
80 |
97 |
76 |
80 |
84 |
54 |
80 |
97 |
76 |
81 |
84 |
56 |
78 |
97 |
76 |
81 |
84 |
58 |
79 |
97 |
78 |
81 |
85 |
60 |
77 |
98 |
79 |
83 |
86 |
Table 2: Validity criteria for OECD 301 F.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
4% |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
Functional control: 86% Functional control with silicone oil: 90% |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
47% |
yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
Functional control: 32% Functional control with silicone oil: 28.6% |
yes |
the pH value should be in the range 6-8.5. |
7.47 – 7.64 |
yes |
Description of key information
Readily biodegradable but failing the 10 -day window (65% and 81% biodegradation after 28 and 60 d respectively, OECD 301 F)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable but failing 10-day window
Additional information
One study is available testing the biodegradability of Hexadecyl(trimethoxy)silane (CAS 16415-12-6) according to the OECD guideline 301 F and GLP standards. Domestic, non-adapted, activated sludge was exposed to 20 mg/L test item for 60 d. Silicone oil was used as a solubilizing agent. A toxicity control containing test substance, reference substance and silicon oil was run in parallel. A biodegradation of 65% and 81% was recorded in the treatments with the test item after 28 and 60 d respectively. However, the 10-day window criterion was not fulfilled. Therefore the substance is readily biodegradable but failing the 10-day window. Furthermore, the substance was not inhibitory to activated sludge microorganisms as shown by the toxicity control (47% degradation of the reference substance after 14 d and 86% after 60 d).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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