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EC number: 610-992-2 | CAS number: 53378-52-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 June - 11 July 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Also according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- GLP compliance:
- yes
Test material
- Reference substance name:
- Sodium O,O-diisobutyl phosphorothioate
- EC Number:
- 610-992-2
- Cas Number:
- 53378-52-2
- Molecular formula:
- C8 H18 O3 P S . Na
- IUPAC Name:
- Sodium O,O-diisobutyl phosphorothioate
- Test material form:
- other: grease-like solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): S10783
- Name: Sodium diisobutyl monothiophoshate
- CAS: 53378-52-2
- Molecular formula: C8H18O3PS.Na
- Molecular weight: 248.26
- Storage condition of test material: not indicated in the study report
- Purity: 70%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations (final test):
Control, 10, 22, 46, 100 and 200 mg/L
- Sampling method:
Samples for possible analysis were taken from all test concentrations and the control according to the schedule below.
Frequency at t=0 h and t=48 h
Volume 1 ml from the approximate centre of the test vessels
- Sample storage conditions before analysis: Samples were stored in a freezer until analysis
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Preparation of test solutions started with a loading rate of 100 mg/l for the combined limit/range-finding test and a loading rate of 220 mg/l for the final test. A short period of vigorous stirring was applied to dissolve the test substance in the test medium. The lower test concentrations were prepared by subsequent dilutions of the 100 and 220 mg/l concentrations, respectively, in test medium. The final test solutions were all clear and colourless.
- Controls: Test medium without test substance or other additives
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: In-house laboratory culture with a known history.
- Age at study initiation (mean and range, SD): With newborn daphnids, i.e. less than 3 days old
- Method of breeding: Acyclical parthenogenesis under specified breeding conditions.
- Feeding during test: None
ACCLIMATION
- Acclimation period: Culture conditions same as test conditions, no acclimation required
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Suspension of fresh water algae
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): Daphnids to be used in the test did not show signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there no delay in the production of the first brood.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- 19.6 - 20.9°C
- pH:
- 6.0 - 8.5
- Dissolved oxygen:
- ≥3 mg/L at the end of the test
- Nominal and measured concentrations:
- Nominal conc.: 0, 10, 22, 46, 100, 220 mg/L
Measured conc.:
At 0 h = 0.0003043 (0), 7.66 (10), 21.8 (22), 51.2 (46), 101 (100) and 222 (220) mg/L
At 48 h = <0.000014 (0), 10.3 (10), 21.7 (22), 44.9 (46), 107 (100) and 240 (220) mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass vessel
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water purified by Reverse Osmosis
- Culture medium different from test medium: No
- Intervals of water quality measurement: pH and dissolved oxigen measured at the beginning and at the end of the test, for all concentrations and the control.
OTHER TEST CONDITIONS
- Photoperiod: 16 h photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility (including mortality) at 24 hours and at 48 hours.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approximately 2
- Range finding study
- Test concentrations: 0.10, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 43-68
- Details on results:
- No immobilisation observed in the control
Analysis of the samples taken at the start of the final test showed that the initial concentrations were in agreement with nominal (99-111%) at nominally 22 mg/l and higher. These concentrations remained stable during the test period (88-108% of initial at the end of the test). The lowest test concentration of 10 mg/l was slightly lower at the start (77-82% of nominal), but again in agreement with nominal at the end of the test period (103% of nominal). Given these results, the effect parameters were based on nominal test concentrations. - Results with reference substance (positive control):
- The actual responses in this reference test with K2Cr2O7 were just outside (below) the ranges of the expected historical responses at the different concentrations, i.e. a 48h-EC50 between 0.3 and 1.0 mg/l. Hence, the sensitivity of this batch of D. magna was slightly higher when compared to the historical data collected at NOTOX.
The 24h-EC50 was 0.49 mg/l with a 95% confidence interval between 0.45 and 0.55 mg/l.
The 48h-EC50 was 0.28 mg/l with a 95% confidence interval between 0.14 and 0.65 mg/l.
Any other information on results incl. tables
Acute immobilisation of daphnids after 24 and 48 hours in the final test
Concentration S-10783 (mg/l) |
Vessel number
|
Number Daphnia exposed |
Response at 24 h |
Response at 48 h |
||
number |
Total % |
number |
Total % |
|||
control |
A B C D |
5 5 5 5 |
0 0 0 0 |
0 |
0 0 0 0 |
0 |
10 |
A B C D |
5 5 5 5 |
0 [2] 0 [2] 0 [4] 0 |
0 |
0 0 0 0 |
0 |
22 |
A B C D |
5 5 5 5 |
0 0 [1] 0 [2] 0 [1] |
0 |
1 0 0 1 |
10 |
46 |
A B C D |
5 5 5 5 |
0 [3] 0 [4] 0 [4] 0 [3] |
0 |
1 [3] 1 [4] 0 [5] 1 [4] |
15 |
100 |
A B C D |
5 5 5 5 |
0 [5] 0 [5] 0 [5] 0 [5] |
0 |
5 [4] 5 [5] 4 [4] 5 [4] |
95 |
220 |
A B C D |
5 5 5 5 |
3 [5] 2 [5] 0 [5] 0 [5] |
25 |
5 5 [3] 5 [1] 5 |
100 |
[ ]: number of daphnids observed trapped at the surface of the test solutions. These organisms were reimmersed into the respective solutions before recording of mobility.
Concentrations of the test substance in test medium - final test | ||||||
Concentration | ||||||
Time of sampling [hours] | Date of sampling [dd-mm-yy] | Date of analysis1[dd-mm-yy] | Nominal [mg/l] | Analysed [mg/l] | Relative to nominal [%] | Relative to initial [%] |
0 | 09/07/2012 | 19/07/2012 | 0 | 0.0003043 | n.a. | |
02 | 0.0002393 | n.a. | ||||
10 | 7.66 | 77 | ||||
102 | 8.19 | 82 | ||||
22 | 21.8 | 99 | ||||
46 | 51.2 | 111 | ||||
100 | 101 | 101 | ||||
220 | 222 | 101 | ||||
48 | 11/07/2012 | 19/07/2012 | 0 | <0.000014 | n.a. | |
10 | 10.3 | 103 | 1305 | |||
22 | 21.7 | 99 | 100 | |||
46 | 44.9 | 98 | 88 | |||
100 | 107 | 107 | 106 | |||
220 | 240 | 109 | 108 |
1) Samples were stored in the freezer (≤ -15°C) until the day of analysis.
2) Reserve sample.
3) A small response in the blank sample, maximum contribution to the 10 mg/l samples is 0.004% based on the response
4) Response smaller then lowest standard taking a dilution factor into account.
5) Relative to the mean concentration of corresponding t=0 samples
n.a.) Not applicable.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study the test material did not induce acute immobilisation of Daphnia magna at 10 mg/L after 48 hours of exposure (NOEC). The 48h-EC50 was 53 mg/L (95% confidence interval between 43 and 68 mg/L).
- Executive summary:
The toxicity of S-10783 to aquatic invertebrates was assessed according to OECD TG 202 and GLP principles. Nominal exposure concentrations were the following: control, 10, 22, 46, 100, 220 mg/L.
The NOEC was 10 mg/L and the EC50 was 53 mg/L (as nominal concentrations).
All criteria for acceptability of the test were met and the present toxicity study is classified as reliable without restrictions according to the OECD guideline No. 202.
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