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EC number: 814-072-3 | CAS number: 77614-79-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 2019 - Oct. 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- July 2011
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Version / remarks:
- January 2012
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,5-bis(difluoromethyl)-1H-pyrazole
- EC Number:
- 814-072-3
- Cas Number:
- 77614-79-0
- Molecular formula:
- C5H4F4N2
- IUPAC Name:
- 3,5-bis(difluoromethyl)-1H-pyrazole
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 32, 10, 3.2 and 1.0 mg test item/L
- Sampling method: Duplicate samples from all test concentrations and the control at the start and every 24 hours until the end of the experiment.
- Sample storage conditions before analysis: Storage in a freezer (<= -20°C) in the dark until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of a 100 mg/L stock solution in test medium. Lower concentrations were prepared by dillution of the stock solution.
- Test medium: Reconstituted Water (OECD Medium)
- Controls: Yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Strain No. 61.81 SAG,
- Source (laboratory, culture collection): in-House Breeding, originally supplied by Sammlung von Algenkulturen, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen"
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Calculated water hardness of the test water: 0.24 mmol/L (= 24 mg/L) as CaCO3
- Test temperature:
- 22.2 - 23.5 °C
- pH:
- The pH value of untreated test water was checked and in range of 8.1 ± 0.1 at test start.
The pH in the control was: 8.0 at test start, 9.1 after 72 hours of exposure and 10.0 at test end The pH in the control medium increased by 1.1 units over the first 72 h. Over the entire test duration of 96 h, an increase of 2.0 units was observed in the control media.
The pH at different test item concentrations was: 7.8 to 7.9 at test start, 7.0 to 9.1 after 72 hours of exposure and 4.7 to 10.0 at test end - Nominal and measured concentrations:
- Nominal concentrations: 100, 32, 10, 3.2 and 1.0 mg test item/L and a control
Measured concentrations (geometric mean): 72 hours: 66.0, 15.5, 2.65, 0.595 and 0.195 mg test item/L and a control
The initial concentrations and the maintenance of the exposure concentrations during the test were determined in the analytical part. All reported results refer to geometric mean measured values since the concentrations of the test item were not within ± 20 % of the measured initial concentrations during the test. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): open covered with air permeable glass dishes
- Material, size, headspace, fill volume: Size of 200 mL volume filled with approximately 100 mL of test medium (≤ 50% of flask volume)
- Aeration: none
- Initial cells density: 10'000 cell/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted Water (OECD Medium)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Continuous illumination
- Light intensity: 4691 lx (range: 4450 to 5060 lx)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer (at 0, 24, 48, and 72 h)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.16 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 47.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % conf. interval
- Remarks:
- 45.6 - 49.7 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.65 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 11.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % conf. interval
- Remarks:
- 10.6 - 12.9 mg/L
- Results with reference substance (positive control):
- 72-h EC50 (Growth rate) Potassium dichromate = 0.941 mg/L
72-h EC50 (Biomass) Potassium dichromate = 0.494 mg/L
72-h EC50 (Yield) Potassium dichromate = 0.445 mg/L
Any other information on results incl. tables
Table 1: Growth Rates µ and Percentage Inhibition of µ during the Test Period
Nominal test |
Growth rate (1/day) 0 - 24 h |
Inhibition growth rate 0 - 24 h in % |
Significance |
Growth rate (1/day) 24 - 48 h |
Inhibition growth rate 24 - 48 h in % |
Significance |
Growth rate (1/day) 0 - 24 h |
Inhibition growth rate 48 - 72 h in % |
Significance |
Control |
1.823 |
- |
|
1.837 |
- |
|
1.675 |
- |
|
1 |
1.757 |
3.7 |
|
1.812 |
1.4 |
|
1.659 |
1 |
|
3.2 |
1.805 |
1 |
|
1.837 |
0 |
|
1.682 |
-0.4 |
|
10 |
1.807 |
0.9 |
|
1.797 |
2.1 |
|
1.668 |
0.4 |
|
32 |
1.694 |
7.1 |
* |
1.615 |
12.1 |
* |
1.422 |
15.1 |
* |
100 |
1.277 |
30 |
* |
0.93 |
49.4 |
* |
0.64 |
61.8 |
* |
negative values in ‘% inhibition’ indicate an increase in growth relative to that of the control
* mean value significantly different from the control (tested with Williams t-test, α = 0.05, one-sided)
Table 2: Validity criteria for OECD 201
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
153 |
Yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
17.1 % |
Yes |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. |
1.7 % |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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