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EC number: 701-337-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 12, 1988 – October 7, 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test was conducted according to OECD Test Guideline No. 471, 1983, under GLP Standards, and QA. Chemical identity and purity of the test substance are not reported. One strain not tested.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1983
- Deviations:
- yes
- Remarks:
- Only four Salmonella test strains
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- yes
- Remarks:
- EU Method B.14 (Other effects-Mutagenicity: Salomonella typhimurium - Reverse Mutation Assay, 1984)
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Remarks:
- Statement of Compliance
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-[(diphenoxyphosphoryl)oxy]phenyl diphenyl phosphate
- EC Number:
- 701-337-2
- Cas Number:
- not available
- Molecular formula:
- C30H24O8P2
- IUPAC Name:
- 3-[(diphenoxyphosphoryl)oxy]phenyl diphenyl phosphate
- Details on test material:
- - Name of test material (as cited in study report): CR 733-S
- Physical state: no data
- Lot/batch No.: confidential
- Storage condition of test material: At room temperature in the dark
Constituent 1
Method
- Target gene:
- His-gene: Amino acid histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- other: S. typhimurium TA98 and TA100: pKM101. All strains: rfa, gal, chl, bio, uvrB
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S9 induced by Aroclor 1254
- Test concentrations with justification for top dose:
- 100, 333, 1000, 3330, and 5000 μg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: -S9-mix: TA1535: sodium azide; TA1537: 9-aminoacridine; TA98: daunomycine; TA100: methylmethanesulfonate. +S9-mix: all strains: 2-aminoanthracene.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Selection time (if incubation with a selection agent): 48 hours
SELECTION AGENT (mutation assays): agar containing Histidine
NUMBER OF REPLICATIONS: 3 plates per concentration
NUMBER OF CELLS EVALUATED: 1 x 10*9 viable cells
DETERMINATION OF CYTOTOXICITY
- Method: Viability determination in non-selective agar: the percentage survival of the TA100 culture is determined by comparing the number of colonies on the solvent control plate with those on the plates containing the test substance, both in the absence and presence of S9-mix.
OTHER EXAMINATIONS:
Preliminary toxicity at 1.0, 3.3, 10, 33.3, 100, 333, 1000, 3330, and 5000 ug/plate.
Plate incorporation: independently repeated experiment. - Evaluation criteria:
- A test substance is considered negative (not mutagenic) in the Ames test if:
a) The total number of revertants in any test strain at any concentration is not greater than two times the solvent control value, with or without metabolic activation.
b) The negative response should be reproducible in at least one independently repeated experiment.
A test substance is considered positive (mutagenic) in the Ames test if:
a) It induces at least a 2-fold, dose-related increase in the number of revertants with respect to the number induced by the solvent control in any of the test strains, either with or without metabolic activation. However, any mean plate count of less than 20 is considered to be not significant. If the test substance shows in the first test only a positive response at one or two concentrations, the assay is repeated with doses just below and exceeding those showing positive effects in the first test.
b) The positive response should be reproducible in at least one independently repeated experiment. - Statistics:
- Mean and Standard Deviation
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: The test substance was not toxic towards the test strain TA100 in the preliminary toxicity test, both in the absence and presence of S9-mix. Therefore 5000 ug/plate was chosen as the top dose level in the mutation tests.
COMPARISON WITH HISTORICAL CONTROL DATA: The negative and strain-specific positive control values fell within the laboratory background historical ranges. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Not relevant
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
All bacterial strains showed negative responses over the entire dose range of the test substance. Based on the results of this study it is concluded that the test substance can be considered as not mutagenic in the Ames Salmonella/microsome assay. - Executive summary:
CR 733-S was tested in the Salmonella/microsome plate test at 100, 333, 1000, 3330, and 5000 ug/plate in the absence and presence of S9-mix. The test substance did not induce a dose-related increase in the number of revertant (His+) colonies in each of the four test strains (S. typhimurium TA1535, TA1537, TA98, and TA100). These results were confirmed in an independently repeated experiment. The test substance can, therefore, be considered as not mutagenic in this test system.
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