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EC number: 700-235-5 | CAS number: 174899-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study Initiation: January 18, 2018 Experiment Start Date: January 23, 2018 Experiment Termination Date: January 25, 2018 Study Termination (Draft Report): February 20, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)
- Version / remarks:
- OECD Guideline for testing of chemicals: Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage OECD 492, last version from 9 October 2017
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-ethyl-3-methyl-1H-imidazol-3-ium; trifluoro[(trifluoromethanesulfonylazanidyl)sulfonyl]methane
- EC Number:
- 700-235-5
- Cas Number:
- 174899-82-2
- Molecular formula:
- C8H11F6N3O4S2
- IUPAC Name:
- 1-ethyl-3-methyl-1H-imidazol-3-ium; trifluoro[(trifluoromethanesulfonylazanidyl)sulfonyl]methane
- Test material form:
- liquid
- Details on test material:
- clear liquid, colorless
Constituent 1
- Specific details on test material used for the study:
- Test Item Name 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide
Synonym EMIM-TFSI; EMIM-NTf2
Molecular weight 391.31 g/mol
Molecular Formula C8H11F6N3O4S2
CAS No 174899-82-2
EC-No 700-235-5
Lot No. 25PI208_12
Appearence colourless clear liquid
Purity > 99 %w
Homogenity homogenous
Production Date 27/04/2017
Expiry Date 05/2019
Storage Conditions: at 20±5°C, kept away from humidity
Test animals / tissue source
- Species:
- human
- Details on test animals or tissues and environmental conditions:
- The EpiOcular™ human cell construct for eye irritation testing (OCL-200-EIT) (Lot No. 27020) was obtained from MatTek In Vitro Life Science Laboratories, SR.
Standard Assay Kit Components (OCL-200-EIT)
1 Sealed 24-well plate (EPI-200) Contains 24 tissues in cell culture inserts on agarose
2 24-well plates sterile (Falcon) Used for MTT viability assay
6 6-well plates sterile (Falcon) Used for maintaining tissues during assay
1 bottle, 200 mL Assay medium (OCL-200-ASY) DMEM based medium
1 bottle, 100 mL DPBS Rinse Solution (TC-PBS) Used for wetting and rinsing the inserts
Test system
- Controls:
- yes, concurrent positive control
- yes, concurrent negative control
- Duration of treatment / exposure:
- 30- min exposure
- Duration of post- treatment incubation (in vitro):
- 2-hour post-incubation after exposure
- Number of animals or in vitro replicates:
- Two tissue replicates
Results and discussion
In vitro
Results
- Irritation parameter:
- other: relative tissue viability
- Remarks:
- determined by the MTT reduction assay
- Value:
- 101.4
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
Any other information on results incl. tables
Eye irritation potentialof1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imideafter 30-min exposure in human model EpiOcularTM
Test item |
OD |
SD |
Viability |
SD |
in vivo |
|
mean |
of OD |
mean (%) |
of viabilities |
prediction |
Negative controla |
1.940 |
0.008 |
100.0 |
0.44 |
NI |
Positive controlb |
0.904 |
0.021 |
46.6 |
1.11 |
I |
1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide |
1.966 |
0.047 |
101.4 |
2.42 |
NI |
a H2O
b methyl acetate
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test item 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide was examined for in vitro eye irritation in human model EpiOcularTM. The magnitude of viability was quantified by using MTT test.
Validity of the test method was ascertained by positive control methyl acetate. Two tissue replicates were used for each treatment (exposure time 30 minutes), including negative and positive controls.
The tissue viability met the acceptance criterion (mean OD of negative control was 1.940). The viability of culture treated by positive control methyl acetate was 46.6%. The positive control met the acceptance criterion: mean tissue viability less than 50%.
Determined viability of culture treated by 1-Ethyl-3-methylimidazolium bis (trifluoromethylsulfonyl)imide (101.4%) fulfilled the criteria for irritancy.
Therefore, the test item 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide is considered to be non irritant to the eye. - Executive summary:
The test item 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide was examined for eye irritation potential in EpiOcularTMEye Irritation Test (OCL-200-EIT).
The irritation potential of the test item was assessed in compliance with: OECD Guideline for testing of chemicals: Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals not Requiring Classification and Labelling for Eye Irritation or Serious EyeDamage OECD 492
and
Protocol: EpiOcular™ Eye Irritation Test (OCL-200-EIT) -For the prediction of acute ocular ]irritation of chemicals; for use with MatTek Corporation’s Reconstructed Human EpiOcularTMModel.
Two tissue replicates were used for each treatment (exposure time of 30 min), including negative and positive controls. The magnitude of viability was quantified by MTT test. Validity of the test method was ascertained by a positive control.
The tissue viability met the acceptance criterion (mean OD of negative control was 1.94). The viability of culture treated by positive control methyl acetate was 46.6%. The positive control met the acceptance criterion: mean tissue viability less than 50%.
The viability of culture treated by 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide was 101.4%.
Based on the results of the study, the test item 1-Ethyl-3-methylimidazolium bis (trifluoromethylsulfonyl)imide according to Evaluation criteria and Acceptance criteria is considered to be non-irritant (NI).
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