methyl (2E)-{2-[(2,5-dimethylphenoxy)methyl]phenyl}(methoxyimino)ethanoate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study conducted in accordance with GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): MEOE
- Test-substance No.: 04/0450-2
- Analytical purity: 96% (analytical report No.: 06L00055)

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Strain: HanRcc:WIST(SPF)
- Source: RCC Ltd Laboratory Animal Services
- Age at study initiation: male animals approx. 8-10 weeks, female animals approx. 12-14 weeks
- Weight at study initiation: males 247 g, females 229 g (mean)
- Housing: Single in stainless steel wire mesh cages, type DK-III
- Diet (ad libitum): Kliba-Labordiaet, Provimi Kliba SA, Kaiseraugst, Basel, Switzerland
- Water (ad libitum): Tap water
- Acclimation period: at least 5 days for administration


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: 0.5% CMC-solution (cleaned sodlum carboxymethylcellulose) in doubly distilled water

Details on dermal exposure:

TEST SITE
- Area of exposure: About 40 cm2
- Type of wrap if used: The bandage consisted of four Iayers absorbent gauze, and Fixomull stretch (adhesive fleece)


REMOVAL OF TEST SUBSTANCE
- Washing: Rinsing of the application site with warm water.
- Time after start of exposure: 24 h

Duration of exposure:

24 h

Doses:

2000 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Individual body weights shortly before application (day 0), weekly thereafter and at the end of the study; Recording of signs and symptoms several times on the day of application, at least once each workday for the individual animals; A check for any dead or moribund animal was made twice each workday and once on Saturdays, Sundays and on public holidays; Necropsy with gross-pathology examination on the last day of the observation period after killing with CO2.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No mortality occurred.

Clinical signs:

No systemnic clinical observations were observed during clinical examination. No local effects were observed.

Body weight:

The mean body weights of the animals increased throughout the study period.

Gross pathology:

No macroscopic pathologic abnormalities were noted in the animals (5 males and 5 females) examined at termination of the study.

Any other information on results incl. tables

Under the conditions of this study the acute dermal median lethal dose (LD50) of MEOE after dermal application was found to be greater than 2000 mg/kg body weight in male and female rats.