o-Biphenyl-dimethylfluorenyl-amin

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09.12.14

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

column elution method

Key result

Water solubility:

< 0.015 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

1 g/L

Incubation duration:

47 h

Temp.:

20 °C

pH:

> 5.4 - < 5.7

Column 12.5 mL/h:

Rotation period of the fraction collector: 90 min per fraction

Duration of sampling: about 48 h (32 fractions) 

 

Fraction no.	V in mL	pH	water solubility in µg/L
26	18.5	5.4	< 14.5
27	18.5	5.6	< 14.5
28	18.5	5.6	< 14.5
29	18.0	5.6	< 14.5
30	18.0	5.7	< 14.5

 

Evaluation of the fractions 26 to 30: The water solubility was found to be below the detection limit of 14.5 ug/L.

 

Column 25.0 mL/h:

Rotation period of the fraction collector: 45 min per fraction Duration of sampling: about 47 h (63 fractions) 

 

Fraction no.	V in mL	pH	water solubility in µg/L
48	17.5	5.7	< 14.5
49	17.5	5.7	< 14.5
50	17.5	5.7	< 14.5
51	17.5	5.7	< 14.5
52	17.0	5.7	< 14.5

 

Evaluation of the fractions 48 to 52: The water solubility was found to below the detection limit of 14.5 µg/L.

In both columns the concentration of five consecutive fractions was constant within ± 30 %. No increasing or decreasing tendency was observed.

The water solubility of the test item (mean value of the columns) was be below the detection limit of 14.5 µg/L.

Conclusions:

The water solubility of the test item at a temperature of 20 °C was determined to be below 0.015 mg/L.

Executive summary:

The water solubility was determined according to OECD 105 using the column elution method with HPLC analysis. 104.3 mg of the test item were dissolved in 100 mL acetone. For charging the columns in each case 25 mL of the test item solution was added to about 3 g of sea sand and the mixtures were shaken. The solvent was then removed under reduced pressure using a rotary evaporator (50 °C, approx. 20 mbar). The dry residues were placed into the columns of the test apparatus, which have afterwards been filled with water (test temperature: 20 °C; water pH at 23 °C: 5.5). After a swelling time of 2 h pumps were started to rinse the test item coated sand with distilled water. The values for the water solubility were calculated from fractions 26 to 30 for the 12.5 mL/h column and from the fractions 48 to 52 for the 25.0 mL/h column. The water solubility was found to be below the quantification limit of 14.5 µg/L. Thus, the water solubility of the test item was determined to be below 0.015 mg/L at 20 °C.

Description of key information

The water solubility of the test item at a temperature of 20 °C was determined to be below 0.015 mg/L (reference 4.8 -1).

Additional information

The water solubility was determined according to OECD 105 using the column elution method with HPLC analysis (reference 4.8 -1). 104.3 mg of the test item were dissolved in 100 mL acetone. For charging the columns in each case ,25 mL of the test item solution was added to about 3 g of sea sand and the mixtures were shaken. The solvent was then removed under reduced pressure using a rotary evaporator (50 °C, approx. 20 mbar). The dry residues were placed into the columns of the test apparatus, which have afterwards been filled with water (test temperature: 20 °C; water pH at 23 °C: 5.5). After a swelling time of 2 h pumps were started to rinse the test item coated sand with distilled water. The values for the water solubility were calculated from fractions 26 to 30 for the 12.5 mL/h column and from the fractions 48 to 52 for the 25.0 mL/h column. The water solubility was found to be below the quantification limit of 14.5 µg/L. Thus, the water solubility of the test item was determined to be below 0.015 mg/L at 20 °C.