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EC number: 689-188-9 | CAS number: 149343-84-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1 - 5 June 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- OECD Guidelines for Testing of Chemicals (1984) No. 203 "Fish, Acute Toxicity Test"
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Method C.1 of Commission Directive 84/449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC) .
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- No further details specified in the study report.
- Analytical monitoring:
- yes
- Details on sampling:
- Ensured by daily renewal of the test media and verified by chemical analysis of the control and the 100 mg/ 1 test groups at 0, 24 and 96 hours.
- Vehicle:
- no
- Details on test solutions:
- Direct dispersion in water. 4.00 g of test material was dispersed in water with the aid of ultrasonic disruption and the volume adjusted to 2 litres to give a 4.00 g/l stock solution. 500 ml of this stock solution was dispersed in dechlorinated tap water and the volume adjusted to 20 litres to give a 100 mg/1 test concentration.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Name: Rainbow trout (Oncorhynchus mykiss).
Source: Parkwood Trout Farm, Wigmore, Kent, U.K.
Acclimatisation: Stock held since 13 May 1993 and acclimatised to test conditions from 25 May 1993.
The stock fish were maintained in a glass fibre tank with a “Single pass” water renewal system. The lighting was controlled to give a 16 hours light and 8 hours darkness cycle.
Temperature: 14.0 °C.
Dissolved Oxygen: ≥ 9.9 mg O2/l
Feeding: Commercial trout pellets daily. Discontinued 24h prior to test.
Mortalities: Zero in the 7 days prior to the start of the test.
Medication: None.
Mean standard length: 4.0 cm (SD = 0.2 cm).
Mean weight: 0.78 g (SD = 0.11 g). - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No post exposure observation period specified in the study report.
- Hardness:
- approximately 50 mg/l as CaCo3.
- Test temperature:
- 14.0 °C.
- pH:
- 7.3 - 7.5
- Dissolved oxygen:
- 9.9 - 10.0 mg O2/l
- Salinity:
- Not applicable-freshwater study
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Verification of test concentrations showed the measured test concentrations from analysis at 0, 24 and 96 hours to be near nominal. It was therefore considered justifiable to estimate the LC50 values in terms of nominal test concentrations only.
- Details on test conditions:
- Test vessels: Glass aquaria holding 20 1 of test media.
Experimental design: 1 test concentration in duplicate plus 1 control. 10 animals per replicate.
This experimental design conforms to a “Limit Test" to confirm that at the maximum test concentration given in the OECD/EEC test guidelines, no mortalities or adverse reactions to exposure were observed.
Method of initiation: Fish were placed, at random, in prepared test media.
Loading: 0.39 g bodyweight/ litre.
Photoperiod: 16 h light: 8 h dark.
Temperature: 14.0 °C.
Aeration: via narrow bore glass tubes.
Test concentration: 100 mg/l in duplicate. This test concentration was assigned following a preliminary range-finding study, where 3 fish were exposed to each of the following concentrations for a period of 96 hours: 1.0, 10 and 100 mg/l. The results showed no mortalities at all the test concentrations, thereby indicating the choice of test concentration employed.
Medium renewal: Daily
Duration of exposure: 96 hours.
Environmental Measurements: pH, dissolved oxygen concentration and temperature were recorded daily throughout the study.
Criteria of death: Absence of (i) respiratory movement and (ii) response to a physical stimulation. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- There were no mortalities or other adverse reactions to exposure in 20 fish exposed to a test concentration of 100 mg/l for a period of 96 hours.
Inspection of the mortality data gave the following results based on nominal test concentrations:
Time (h) LC50 (mg/l)
3 >100
6 >100
24 >100
48 >100
72 >100
96 >100
Highest test concentration resulting in 0% mortality ≥ 100 mg/l
Lowest test concentration resulting in 100% mortality > 100 mg/l
"No Observed Effect Concentration" (NOEC) ≥ 100 mg/l
The "No Observed Effect Concentration" is based upon zero mortalities and the absence of any behavioural reactions to exposure at this concentration.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l. - Results with reference substance (positive control):
- Not specified
- Sublethal observations / clinical signs:
Cumulative Mortality Data for Rainbow Trout Exposed for 96 hours to JPR Blue 100
Nominal Concentration (mg/l)
Cumulative mortality (initial population: 10)
3 h
6 h
24 h
48 h
72 h
96 h
Control
0
0
0
0
0
0
100 R1
0
0
0
0
0
0
100 R2
0
0
0
0
0
0
R1– R2= Replicates 1 and 2
Environmental Measurements for Rainbow Trout Exposed for 96 Hours to JPR Blue 100
Nominal Concentration (mg/l)
0 hours
24 hours
48 hours
72 hours
96 hours
pH
mg O2/l
T °C
pH
mg O2/l
T °C
pH
mg O2/l
T °C
pH
mg O2/l
T °C
pH
mg O2/l
T °C
Control
7.3
9.9
14.0
7.4
9.9
14.0
7.5
10.0
14.0
7.4
10.0
14.0
7.4
10.0
14.0
100 R1
7.4
9.9
14.0
7.5
10.0
14.0
7.5
9.9
14.0
7.4
10.0
14.0
7.4
9.9
14.0
100 R2
7.4
9.9
14.0
7.5
10.0
14.0
7.5
9.9
14.0
7.5
9.9
14.0
7.4
10.0
14.0
R1– R2= Replicates 1 and 2
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The following LC50 values are based on nominal test concentrations
Time (h) LC50 (mg/l)
3 >100
6 >100
24 >100
48 >100
72 >100
96 >100
"No Observed Effect Concentration" (NOEC) ≥ 100 mg/l - Executive summary:
TEST SPECIES: Rainbow trout (Oncorhynchus mykiss)
TEST TYPE: Acute toxicity. 96 h LC50
TEST CONDITIONS: Semi-static test conditions according to OECD Guideline No. 203 referenced as Method C.1 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/548/EEC).
The following LC50 values are based on nominal test concentrations
Time (h) LC50 (mg/l)
3 >100
6 >100
24 >100
48 >100
72 >100
96 >100
"No Observed Effect Concentration" (NOEC)≥100 mg/l
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.
Reference
Description of key information
The following values are based on nominal test concentrations
96h LC50: >100 mg/l
"No Observed Effect Concentration" (NOEC )≥100 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
TEST SPECIES: Rainbow trout (Oncorhynchus mykiss)
TEST TYPE: Acute toxicity. 96 h LC50
TEST CONDITIONS: Semi-static test conditions according to OECD Guideline No. 203 referenced as Method C.1 of Commission Directive 84/449/EEC (which constitutes Annex V of Council Directive 67/548/EEC) .
The following LC50 values are based on nominal test concentrations
Time (h) LC50 (mg/l)
3 >100
6 >100
24 >100
48 >100
72 >100
96 >100
"No Observed Effect Concentration" (NOEC)≥100 mg/l
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.
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