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EC number: 210-764-9 | CAS number: 623-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-01-13 - 1997-01-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 Part L8
- Version / remarks:
- 03-1991
- Qualifier:
- according to guideline
- Guideline:
- other: NEN-EN-ISO 10712
- Version / remarks:
- 1996
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-bromobenzonitrile
- EC Number:
- 210-764-9
- EC Name:
- 4-bromobenzonitrile
- Cas Number:
- 623-00-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 4-bromobenzonitrile
- Reference substance name:
- 2-bromobenzonitrile
- EC Number:
- 218-045-1
- EC Name:
- 2-bromobenzonitrile
- Cas Number:
- 2042-37-7
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 2-bromobenzonitrile
- Reference substance name:
- 3-bromobenzonitrile
- EC Number:
- 230-127-9
- EC Name:
- 3-bromobenzonitrile
- Cas Number:
- 6952-59-6
- Molecular formula:
- C7H4BrN
- IUPAC Name:
- 3-bromobenzonitrile
- Reference substance name:
- Benzonitrile
- EC Number:
- 202-855-7
- EC Name:
- Benzonitrile
- Cas Number:
- 100-47-0
- Molecular formula:
- C7H5N
- IUPAC Name:
- benzonitrile
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Water
- Test material form:
- solid
Constituent 1
impurity 1
impurity 2
impurity 3
impurity 4
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: Psreudomonas putida
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain:
- Source (laboratory, culture collection):
- Age of inoculum (at test initiation):
- Method of cultivation:
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 16 h
Test conditions
- Test temperature:
- 23 +/- 1
- pH:
- 7.1 - 7.4
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glas, 100 mL, ./. 40 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): na
- Renewal rate of test solution (frequency/flow rate): na
- Initial cells density: FNU/436 nm = 50
- Control end cells density:
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): 0
GROWTH MEDIUM
- Standard medium used: no
- Detailed composition if non-standard medium was used:
Solution I: 10.0 g NaNO3
2.4 g K2HPO4
1.2 g KH2PO4
1.0 g yeast extract
Dissolved in 500 mL water
Silution II: 10.0 g NaNO3
2.4 g K2HPO4
1.2 g KH2PO4
Dissolved in 500 mL water
Solution III: 40.0 g D(+)-glucose monohydrate
Dissolved in 500 mL water
Solution IV: 0.01 g Fe(III)citrate
4.0 g MgSO4 * 7 H2O
Dissolved in 1 L water
test medium: 25 mL I
25 mL III
50 mL IV
made up to 1 L with water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
tab water purified by reverse osmosis and subsequently passed over activated carbon and ion-exchange cartiges
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile test conditions:
- Adjustment of pH: 7.1 - 7.4
- Photoperiod:
- Light intensity and quality:
- Salinity (for marine algae):
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer
- Chlorophyll measurement: na
- Other:
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline:
- Range finding study na
- Test concentrations (mg/L): 0; 6.3; 12.5; 25; 50; 100; 200
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 57 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 135 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 22 mg/L
- Other: - Reported statistics and error estimates:
- Linear regression
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the result of the test presently performed, the toxicity threshold value (EC10) of 4-Bromobenzonitrile was 57 mg/L. The "Bewertungszahl" is therefore 4.2 (Bewertung wassergefährdender Stoffe, 1989).
The EC50 was calculated to be 135 mg/L. - Executive summary:
4 -Bromobenzonitril was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L 8 (March 1991) and NEN-EN-ISO 10712 (1996).
Suspensions of Speudomonas putida were exposed th concentrations ranging from 6.3 to 200 mg/L 4 -Bromobenzonitrile.
After incubation for 16 h the extinction values of the bacterial cell suspensions were measured. Significant (> 10 %) toxicity was observed at 100 and 200 mg/L.
Based on the result of the test presently performed, the toxicity threshold value (EC10) of 4-Bromobenzonitrile was 57 mg/L. The "Bewertungszahl" (Assessment figure or value for bacterial toxicity) is therefore 4.2 (Bewertung wassergefährdender Stoffe, 1989). The EC50 was calculated to be 135 mg/L.
The EC50 value for the reference substance, 3,5 -dichlorophenol, tested during the same experiment, was 22 mg/L. Since this value was within the eccepted range of 10 - 30 mg/L for the EC50, and the multiplication factor of the bacteria within the test was over 60, it was concluded that the test conditions were optimal and the results obtained were valid.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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