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EC number: 942-299-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 10, 2015 - March 13, 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Remarks:
- The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (< 1 mg/L), the compound cannot be detected with standard analytical methods.
- Vehicle:
- yes
- Remarks:
- reconstituted water
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- The test medium (reconstituted water and test item) was prepared freshly. Therefore, the calibrated tlask with test medium was treated in an ultrasonic device for 1 hour. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the forrnulation was passed through a membrane filter with a pore size of 0.2 μm. The filtrate was used for the study. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna Straus
- Source: IBACON GmbH (Roßdorf, Germany)
- Age of parental stock (mean and range, SD): Parental animals were used until they were about 6 weeks old.
- Feeding during test: no
ACCLIMATION
- Acclimation period:To avoid an acclimation phase before start of the study, reconstituted water was used during culture as weil as during the study.
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: ad libitum with unicellular green algae Desmodesmus subspicatus
- Feeding frequency: three times per week
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Before start of the experimental phase, the young daphnids were separated from their different parent animals and put into a collective test vessel containing reconstituted water. The daphnids were visually inspected for motility. If daphnids did not make any swimming movements within 15 seconds after their test vessels have been gently agitated, they were considered to be immobile and were not used for the study. At the start of the experimental phase the daphnids were cautiously removed using a pipette, separated from the reconstituted water using a finemesh sieve, and placed into the test vessels containing test media (test item group) or reconstituted water (control group). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- about 250 mg/L CaCO3
- Test temperature:
- 21.1 - 21.8°C
- pH:
- 7.70 - 7.73
- Dissolved oxygen:
- 8.07 - 8.16 O2 mg/L
- Salinity:
- NA
- Nominal and measured concentrations:
- 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessels
- Type: open
- Material, size, headspace, fill volume: glass, fill volume: 20 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
According to ELENDT M4 medium. After preparation the reconstituted water was aerated for 24 hours. Hardness: about 2.5 mmol/L (about 250 mg/L CaC03), pH: 7.9 ± 0.3, after an aeration of 24 hours.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours dark
- Light intensity: 667 - 668 Lux
EFFECT PARAMETERS MEASURED: mobility after 24 and 48 hours
VEHICLE CONTROL PERFORMED: yes
- Reference substance (positive control):
- no
- Remarks:
- No positive control used in this study. The accuracy and reliability of the test method is demonstrated periodically as recommended by guidelines with potassium dichromate.
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- EC50 > maximum solubility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other:
- Remarks:
- EC50 > maximum solubility
- Details on results:
- - Mortality of control: no
- Other adverse effects control: no - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of revealed no aquatic toxicity in the test system. The 48h EC50 exceeded the maximum solubility (nominal > 100 mg/L) and, thus, could not be determined in this study.
- Executive summary:
The objective of this study was to evaluate the influence of the test item on the immobilization of Daphnia magna.
For this purpose, juvenile daphnids were exposed to a nominal test item concentration of 100 mg/L (limit test) over 48 hours, in an open static system. The daphnids were observed for immobilization 24 and 48 hours after placing in the aqueous test item solution. The study comprised of one test item group with four test vessels containing five daphnids each, i.e. 20 daphnids in total. Additionally, one control group (20 daphnids) was used.
The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (< 1 mg/L), the compound cannot be detected with standard analytical methods and the development of an analytical method with a sufficiently low detection and quantification limit is not justified.
Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
Daphnids exposed to an aqueous preparation of a nominal concentration of 100 mg/L of the test item were not affected.
The following EC50 values for daphnids were determined:
24h EC50: > maximum solubility (nominal> 100 mg/L)
48h EC50: > maximum solubility (nominal > 100 mg/L)
The 48h EC50 exceeded the maximum solubility (nominal > 100 mg/L) and, thus, could not be determined in this study.
Reference
Description of key information
Under the conditions of the present study, an aqueous solution of nominal 100 mg/L of revealed no aquatic toxicity in the test system. The 48h EC50 exceeded the maximum solubility (nominal > 100 mg/L) and, thus, could not be determined in this study.
Key value for chemical safety assessment
Additional information
The objective of this study was to evaluate the influence of the test item on the immobilization of Daphnia magna.
For this purpose, juvenile daphnids were exposed to a nominal test item concentration of 100 mg/L (limit test) over 48 hours, in an open static system. The daphnids were observed for immobilization 24 and 48 hours after placing in the aqueous test item solution. The study comprised of one test item group with four test vessels containing five daphnids each, i.e. 20 daphnids in total. Additionally, one control group (20 daphnids) was used.
The test item concentration in the reconstituted water was not quantified at the start and the end of this study. Because of the low water solubility (< 1 mg/L), the compound cannot be detected with standard analytical methods and the development of an analytical method with a sufficiently low detection and quantification limit is not justified.
Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
Daphnids exposed to an aqueous preparation of a nominal concentration of 100 mg/L of the test item were not affected.
The following EC50 values for daphnids were determined:
24h EC50: > maximum solubility (nominal> 100 mg/L)
48h EC50: > maximum solubility (nominal > 100 mg/L)
The 48h EC50 exceeded the maximum solubility (nominal > 100 mg/L) and, thus, could not be determined in this study.
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