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Reaction mass of N,N-ethanediylbis- Hexadecanamide, N-[2-[(1-oxohexadecyl)amino]ethyl]-Octadecanamide, Hexadecanoic acid, 12-hydroxystearic acid, reaction products with ethylenediamine, N,N-ethanediylbis-Octadecanamide and Octadecanoic acid, 12-hydroxystearic acid, reaction products with ethylenediamine
EC number: 906-763-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Nov - 18 Nov 2005
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Remarks:
- according to the Japanese GLP Standard
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 146.6 °C
- Water solubility: < 4.9 μg/L
OTHER PROPERTIES
- Other: solubility: < 200 μg/L in hexane, 57 mg/L in chloroform, < 200 μg/L in ethyl acetate, < 200 μg/L in methanol, < 4.9 mg/L in octanol - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 100 mg/L
- Sampling method: test solution for analysis was separately prepared for analysis at the initiation of the test. After exposure, same volume of test solution was sampled directly from each test vessel and cetrifuged (3000 rpm, 10 min) to get rid of alga.
- Sample storage conditions before analysis: analysis was conducted immediately after sampling, no storage - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test item is immiscible with water and therefore a WAF was prepared by adding the test item in dilution water. After 30 minutes sonication, test water was stirred (750 rpm) for 24 hours at 23± 2 °C. Test water was filtered with No. 2 filter paper.
- Controls: dilution water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): test water was colorless after preparation - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: alga
- Strain: ATCC22662
- Source: American Type Culture Collection
- Method of cultivation: on rotary shaker
ACCLIMATION
- Acclimation period: 5 days (11/11/2005 - 15/11/2005)
- Culturing media and conditions (same as test or not): same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 22.0 - 22.7 (23 ± 2 °C)
- pH:
- 7.7 - 9.9
- Nominal and measured concentrations:
- nominal concentration: 100 mg/L
measured concentration : 0.053 (at the initiation), 0.00619 (after exposure) mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flask with an air-permeable stopper
- Type: open
- Material, fill volume: glass, 100 mL
- Aeration: no
- Initial cells density: 1000 cells/mL
- Control end cells density: 2883100 ± 120600
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not stated
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: not adjusted
- Photoperiod: continuously
- Light intensity and quality: 4000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber; electronic particle counter;
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: control, 10.0, 50.0, 100 mg/L
- Results used to determine the conditions for the definitive study: growth inhibition rate: 10.0 mg/l: -8%, 50.0 mg/L: -11%, 100 mg/L: -40% - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (reagent grade)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- refers to N N'-ethylene-bis-octadecanamide
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- refers to N N'-ethylene-bis-octadecanamide
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- refers to N N'-ethylene-bis-octadecanamide
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 39.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Remarks:
- refers to N N'-ethylene-bis-octadecanamide
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): not observed
- Unusual cell shape: not observed
- Flocculation: not observed
- Adherence to test vessels: not observed
- Aggregation of algal cells: not observed - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EbC50: 0.430 ± 0.0698 mg/L (n=17)
- Other: this test is performed every 6 months. - Reported statistics and error estimates:
- The EC50 values and associated 95% confidence limits could not be determined statistically because the test was conducted at one concentration level. The NOEC were determined by Student's t-testm subsequent to F test for homogeneity of variances. Statistical analyses were performed using Yukms Statlight #3 software (Yukms Corp., Tokyo) and all tests of significant were at α=0.05.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- refer to chapter 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the result of a toxicity to algae test with a read across substance it can be concluded that the registered substance exhibited no acute toxicity to the freshwater algae Pseudokirchenriella subcapitata within the range of water solubility.
- Executive summary:
The toxicity of the read Across Source substance Amides, C16-C18 (even), N,N'-ethylenebis to the freshwater algae Pseudokircheneriella subcapitata was investigated in one study from the Japanese Ministry of Environment (MOE, 2002). This study followed the OECD Guideline No. 201: Algae, Growth Inhibition Test (1984). The results from this test show that after 72 hours exposure to Amides, C16-C18 (even), N,N'-ethylenebis, no effects on growth rate of Pseudokircheneriella subcapitata were observed, up to the highest attainable concentration (0.053 mg/L, limit of solubility). Therefore, the 72h-EC50 was greater than 0.053 mg/L and no toxicity within the range of water solubility was reported.
Referenceopen allclose all
Determined effect concentration based on the measured concentration of the test item:
EbC50: > 0.053 mg/L
NOECb: > 0.053 mg/L
ErC50: > 0.053 mg/L
NOECr: > 0.053 mg/L
Determined effect concentration based on the measured concentration of active ingredient (N N'-ethylene-bis-octadecanamide, CAS 110 -30 -5)
EbC50: > 0.0211 mg/L
NOECb: > 0.0211 mg/L
ErC50: > 0.0211 mg/L
NOECr: > 0.0211 mg/L
Table 1. Measured Concentration of the Test Substance in Test Cultures
Loading Rate (*1) (mg/L) |
Measured Concentration (mg/L) (Percent of 0hr) |
|
0 Hour |
72 Hour |
|
Control |
<0.002 |
<0.00002 |
100 |
0.053 |
0.00619 (12) |
(*1): The ratio of test substance to water (in mg/L) used in the preparation of the WAF.
Table 2. Cell Densities of Pseudokirchneriella subcapitata during the 72 -Hour Exposure
Loading Rate [Measured Conc. at 0 hr] (mg/L) |
Cell Densities (cells/mL) |
||||
|
0 Hour |
24 Hour |
48 Hour |
72 Hour |
|
Control |
Average |
10000 |
82800 |
756900 |
2883100 |
SD |
0 |
11900 |
123400 |
120600 |
|
100 [0.053] |
Average |
1000 |
83000 |
778900 |
3413100 |
SD |
0 |
16300 |
138300 |
70200 |
Description of key information
Based on the result of a toxicity to algae test with a read across substance it can be concluded that the registered substance exhibited no acute toxicity to the freshwater algae Pseudokirchenriella subcapitata within the range of water solubility.
The toxicity of the read Across Source substance Amides, C16-C18 (even), N,N'-ethylenebis to the freshwater algae Pseudokircheneriella subcapitata was investigated in one study from the Japanese Ministry of Environment (MOE, 2002). This study followed the OECD Guideline No. 201: Algae, Growth Inhibition Test (1984). The results from this test show that after 72 hours exposure to Amides, C16-C18 (even), N,N'-ethylenebis, no effects on growth rate of Pseudokircheneriella subcapitata were observed, up to the highest attainable concentration (0.053 mg/L, limit of solubility). Therefore, the 72h-EC50 was greater than 0.053 mg/L and no toxicity within the range of water solubility was reported.
Key value for chemical safety assessment
Additional information
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