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EC number: 247-361-2 | CAS number: 25952-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August to October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- White Crystalline powder
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Test type:
- semi-static
- Water media type:
- other: Tap water purified by reverse osmosis
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- 3 hours, during which aeration and stirring took place.
- Post exposure observation period:
- After the 3-hour contact time the oxygen consumption was recorded for a period of approximately 10 minutes.
- Test temperature:
- 20-22 C
- pH:
- 7.7
- Nominal and measured concentrations:
- Five concentrations: 3.2, 10, 32, 100, 320 and 1000 mg/L. 5 replicates per test group and 6 replicates for the control.
- Details on test conditions:
- Contact time: 3 hours, during which aeration and stirring took place.
Vessels: All glass open bottles/vessels.
Milli-RO / Milli-RO water: Tap water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA).
Synthetic medium (=sewage feed): 16 g peptone, 11 g meat extract, 3 g urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4. Dissolved in Milli-RO water, made up to 1 litre
and filtered. The pH was within 7.5 ± 0.5.
Air supply: Clean, oil-free air.
Aeration: The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the sludge flocs in suspension. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 69 - <= 132 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- >= 123 - <= 199 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 347 - <= 470 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (total fraction)
- Basis for effect:
- inhibition of total respiration
- Details on results:
- A combined limit/ Range-finding test was carried out. It showed 11%, 26% and 90% inhibition of the respiration rate at a concentration of 10,100 and 1000mg/l respectively. Therefore, the expected EC50 was between a concentration of 100 and 1000mg/l. There was no oxygen uptake from abiotic processes. All test conditions prescribed by the study plan were met. Although the validity criterion of the mean control oxygen uptake was less than 30 mg O2/L.h (29.28 mg O2/L.h) the study was considered to be valid, since the validity criterion stated in the OECD Guideline (20 mg O2/g.h) was met.
For the final test, The effects observed were in agreement with what was expected based on the results of the combined limit/range-finding test. The results of this test allowed for reliable determination of a NOEC and ECx values. No statistically significant inhibition of the respiration rate of the sludge was recorded at 100 mg 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride per liter (for more information on statistics see Appendix 4). At higher concentrations the inhibitory effect of 1- (3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride on aerobic waste water
(activated sludge) bacteria increased with increasing concentration, with 42% and 80% inhibition of the respiration rate at 320 and 1000 mg/L, respectively. - Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, under the conditions of this present test 1-(3-Dimethylaminopropyl)- 3- ethylcarbodiimide hydrochloride was not toxic to waste water bacteria (activated sludge) at or below a concentration of 100 mg/L (NOEC). The EC10 was 101 mg/L (95% confidence interval: 69 - 132 mg/L). The EC20 was 163 mg/L (95% confidence interval: 123 - 199 mg/L). The EC50 was 403 mg/L (95% confidence interval: 347 - 470 mg/L).
- Executive summary:
The objective of the study was to evaluate 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride for its ability to adversely affect aerobic microbial treatment plants and, if possible, to determine the EC50 and/or the no-observed effect concentration (NOEC). The influence of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The study procedures described in this report were based on the OECD guideline No. 209, 2010. The batch of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride tested was a white crystalline powder with a purity of 99.9% (titrimetric assay). No correction was made for the purity/composition of the test item. The test started with the preparation of a stock solution of 10 g/L. Magnetic stirring was applied to accelerate dissolution and to ensure homogeneity. Volumes of the clear and colourless stock solution corresponding to the test concentration were then added to the test media. Optimal contact between the test item and test medium was ensured by applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was recorded for approximately 10 minutes. The final test was performed based on the result of a preceding combined limit/range-finding test. Six concentrations were tested, ranging from 3.2 to 1000 mg/L and increasing with a factor 3.2. Five replicates per concentration and six replicates for an untreated control group were tested. No statistically significant inhibition of the respiration rate of the sludge was recorded at 100 mg 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride per L. At higher concentrations the inhibitory effect on aerobic waste water (activated sludge) bacteria increased with increasing concentration, with 42% and 80% inhibition of the respiration rate at 320 and 1000 mg/L, respectively. The batch of activated sludge was tested for sensitivity with the reference item 3,5-dichlorophenol, and showed normal sensitivity. The study met the acceptability criteria prescribed by the study plan and was considered valid. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/L (NOEC). The effect parameters for the inhibition of the respiration rate are presented below.
Parameter 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride Concentration (mg/L) NOEC 100 EC10 101 (69 -132mg/L) EC20 163 (123 -199 mg/L) EC50 403 (347 -470 mg/L)
Reference
Description of key information
A combined limit/ Range-finding test was carried out. It showed 11%, 26% and 90% inhibition of the respiration rate at a concentration of 10,100 and 1000mg/l respectively. Therefore, the expected EC50 was between a concentration of 100 and 1000mg/l. There was no oxygen uptake from abiotic processes. All test conditions prescribed by the study plan were met. Although the validity criterion of the mean control oxygen uptake was less than 30 mg O2/L.h (29.28 mg O2/L.h) the study was considered to be valid, since the validity criterion stated in the OECD Guideline (20 mg O2/g.h) was met.
For the final test, The effects observed were in agreement with what was expected based on the results of the combined limit/range-finding test. The results of this test allowed for reliable determination of a NOEC and ECx values. No statistically significant inhibition of the respiration rate of the sludge was recorded at 100 mg 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride per liter. At higher concentrations the inhibitory effect of 1- (3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride on aerobic waste water (activated sludge) bacteria increased with increasing concentration, with 42% and 80% inhibition of the respiration rate at 320 and 1000 mg/L, respectively.
EC50 values are >=347 <=470mg/L
NOEC ca 100 mg/L
EC10 >=69 <=132 mg/L
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
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