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EC number: 606-285-3 | CAS number: 1928-56-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study had been performed in accordance with OECD Guideline No.: 423.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- methyl (2,3-dichlorophenoxy)acetate
- EC Number:
- 606-285-3
- Cas Number:
- 1928-56-9
- Molecular formula:
- C9H8Cl2O3
- IUPAC Name:
- methyl (2,3-dichlorophenoxy)acetate
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): Dichlorophenoxyacetic acid methyl ester
- Physical state: almost white powder
- Purity test date: 26. 09. 2005
- Lot/batch No.: 3
- Expiration date of the lot/batch: : 07. 2006
- Storage condition of test material: at room temperature, protected from light
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: TOXI COOP KKT. Budapest, HUNGARY
- Age at study initiation: 40-45 days
- Weight at study initiation: 131.8-150.5 g
- Fasting period before study: approx. 20 hours (after the test item administration food was withheld for 3 hours)
- Housing: 3 animals / cage, in Techniplast 1291 type plastic cages. (425x266x180 mm)
- Diet (e.g. ad libitum): ad libitum, ssniff SM r/M-z+H complete feed for rats and mice supplied by TOXI COOP KKT.
- Water (e.g. ad libitum): potable water, ad libitum, offered daily in 500-ml drinking bottles sterilized before use (121 °C, 20 minutes)
- Acclimation period: 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20.3-22.6 °C
- Humidity (%): 36-53 %
- Air changes (per hr): 10-15 / h
- Photoperiod (hrs dark / hrs light): 12 h dark / 12 h artificial light
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 1% Methylcellulose
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 2000 mg / kg bodyweight = 20 ml / kg test item in vehicle suspension
3.000 g, 0.450 g dichlorophenoxyacetic acid methyl ester + 1% mucilage methylcellulose ad 30.0 ml - Doses:
- 2000 mg / kg
300 mg / kg - No. of animals per sex per dose:
- 3 at dose 2000 mg/kg
2x3 at dose 300 mg/kg - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: for 6 hours after treatment and then at least twice a day
- Necropsy of survivors performed: yes ( gross)
- Other examinations performed: clinical signs of toxicity, status of skin, fur, eyes, mucous membranes, respiratory, circulatory, autonomic and central nervous system, somatomotor activity, body weight
Results and discussion
Effect levels
- Sex:
- female
- Dose descriptor:
- approximate LD50
- Effect level:
- > 200 - <= 2 000 mg/kg bw
- Mortality:
- All animals given 2000 mg/kg test item died within three days after dosing (Day 1, 2 and 3). No lethalities occurred in the other six animals dosed with 300 mg/kg on the dosing day or during the 14-day observation period.
- Clinical signs:
- other: Before death, somnolence of different degree, incoordinated movement and haemorrhagic nasal discharge were seen in the animals which died (2000 mg/kg). No changes were observed in the appearance and behaviour of animals dosed with 300 mg/kg on the dosing
- Gross pathology:
- The following necropsy findings were found in the died animals (2000 mg/kg): bad condition; disseminated haemorrhages in the thymus; enlarged stomach containing dosing preparation, dark-coloured, point-like deposits, and mucosal erosions in the stomach; red-coloured gaseous, mucous content in the small intestines; mottled lungs with haemorrhages; congested brain.
No macroscopic changes were detected at he necropsy of animals dosed with 300 mg/kg.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the applied test conditions, the approximate calculated cut-off LD50 value of dichlorophenoxyacetic acid methyl ester administered by oral route to Crl:(WI)BR rats was above 300 mg/kg, therefore, according to the requirements of the Minister of Health 44/2000.(XII.27.) EüM regulation the test item could be classified as harmful (>200-2000 mg/kg).
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