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EC number: 612-975-5 | CAS number: 6225-08-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-05-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Specific details on test material used for the study:
- -Test Item Properties
Test item: N,N-Dimethylnonanamide
Trade name: Genagen PA
Batch number: 112-2016-11 D
CAS No.: 6225-08-7
Sum formula: C11H23NO
Molecular weight: 185.31 g/mol
Purity (certified): 98.3% (w/w) (100% minus by-products)
Water solubility: approx. 340 mg/L (20 °C)
Appearance: Yellow clear liquid
Expiry date: May 2018
Recommended storage: Cool and dry (+2-+8 °C)
The test item and the information concerning the test item were provided by the sponsor.
-Test Facility Actions
Receipt: 2016-08-12
Identification parameter: Name, batch number, state, color and turbidity
Retention sample: At least 1 g has been retained on 2016-08-18 and stored at 6 ± 2 °C.
Storage at test facility: 6 ± 2 °C, protected from light in the tightly closed original container - Test temperature:
- The test temperature was measured by a data logger with an interval of 2 minutes.
The mean temperature of 23.9 °C was inside the range of 20 - 25 °C ± 1 °C during the course of the study - Details on study design: HPLC method:
- -Test system: Analytical HPLC-column (based on silica matrix), coupled to DAD, packed with a cyanopropyl solid phase containing non-polar and polar moieties.
-Reasons for the selection: The selection of the test system is based on the guidelines. HPLC coupled to DAD is a useful method for estimation of the adsorption coefficient KOC. The KOC indicates the binding capacity of the test item on soil or sewage sludge and allows comparing the adsorption behaviour of different chemicals. The test item and reference items were detected by DAD.
-HPLC system HPLC AGILENT 1100 consisting of:
Auto sampler G1329A
Auto sampler thermostat G1330B
Pump G1312A
Degasser G1322A
Column oven G1316A
Detector G1315B
-Software: Empower 2 (Build No: 2154), WATERS
-Column: YMC-Pack Cyano, S-5µm, 12 nm, 250 x 4.0 mm, YMC EUROPE GMBH
Mobile phase : Methanol and HPLC water in a ratio 50 : 50. This mobile phase allowed the determination of the adsorption coefficient (KOC) for the test item in a reasonable period with suitable chromatographic properties for the test and reference items. No buffer was used because the adsorption coefficient (KOC) of the test item is independent on the pH value for this test item.
-Additional equipment: Data logger/thermometer TESTO 175-T3
Analytical balance, SARTORIUS
Top pan balance, KERN
Positive displacement pipettes, Microman, GILSON
Piston stroke pipettes, THERMO SCIENTIFIC
Laboratory glassware
-Reagents: HPLC water, for HPLC, batch 17B174005, VWR
Methanol, gradient grade, batch 17C134009, VWR
3-Nitrobenzamide, 98%, batch 53297LJV, ALDRICH
Endosulfan-diol, 99.9%, batch SZBD302XV, SIGMA-ALDRICH
-Column temperature: 20 - 25 ± 1 °C
-Flow rate: 1.0 mL/min
-Injection volume: 20 µL
-Temperature monitoring: Data logger using 2 minutes interval
-System suitability: The following capacity factors k’ were reached:
log k’ > 0 for log KOC = 3.02 (Endosulfan-diol) and
log k’ > -0.4 for log KOC = 1.95 (3-Nitrobenzamide)
by using methanol : HPLC water (55 : 45) as mobile phase. - Key result
- Type:
- log Koc
- Value:
- ca. 2.32 dimensionless
- Temp.:
- 24 °C
- Remarks on result:
- other: for soil
- Validity criteria fulfilled:
- yes
- Remarks:
- Calculated values for log KOC of the test item did not differ by more than 0.25 between injections. The variation in temperature was between ± 1 °C during the course of measurements. A signal-to-noise ratio > 3 was provided for all substances.
- Conclusions:
- The adsorption coefficient log KOC of the test item N,N-Dimethylnonanamide was determined to be 2.32 for soil and 2.35 for sewage sludge.
- Executive summary:
The adsorption coefficient (KOC) for the test itemN,N-Dimethylnonanamide (batch number:112-2016-11D) on soil and on sewage sludge using high performance liquid chromatography (HPLC) was estimated according to OECD/OCDE 121 (2001) and Council Regulation (EC) No. 440/2008, Method C.19 on 2017-05-12 at Noack Laboratorien GmbH, 31157 Sarstedt, Germany.
The test was performed temperature controlled (23.9 °C, mean value), with a mobile phase consisting of 50% methanol and 50% HPLC water. A 50 mg/L sample of the test item
N,N-Dimethylnonanamide was analyzed threefold via HPLC on a cyanopropyl (CN-) column with DAD‑detection.Calibrations were performed using seven reference items for soil and for sewage sludgewith known logKOC values (given by the guidelines). The reference items were measured twice, once before and once after the measurement (double injection of one replicate) of the test item. Dead time of the HPLC-system was determined with sodium nitrate.
Two individual log KOC values for soil and for sewage sludge for the test item were derived under consideration of the first and second calibration. Finally a mean value for soil and for sewage sludge was calculated from these individual logKOC values. The results are shown in the table below.
Log KOC Values forN,N-Dimethylnonanamidein Soil and Sewage Sludge
Test Item
logKOC, soil
logKOC,sewage sludge
1. Calibration
2. Calibration
1. Calibration
2. Calibration
2.31 ± 0.08
2.11 to 2.50(95% CI)
2.32 ± 0.08
2.11 to 2.53(95% CI)
2.35 ± 0.11
2.07 to 2.62(95% CI)
2.36 ± 0.11
2.09 to 2.63(95% CI)
Mean
2.32
2.35
KOC : Adsorption coefficientaccording to the guidelines
CI : Confidence Interval
The adsorption coefficient logKOCfor the test item
N,N-Dimethylnonanamide was calculated to be 2.32 for soil
and 2.35 for sewage sludge.
Reference
Temperature
The test temperature was measured by a data logger with an interval of 2 minutes.
The mean temperature of 23.9 °C wasinside the range of 20 - 25 °C ± 1 °C during the course of the study.Details are given in the table below.
Temperature Range
|
Temperature[°C] |
Min. |
23.7 |
Max. |
24.1 |
Mean Value |
23.9 |
Dead Time of the HPLC-System
The HPLC dead time (t0) was determined by threefold injection of a solution of 5 mg/L NaNO3. The results are provided in the table below.
Dead Time of the HPLC-System
Injection No.
|
t0 [min] |
1 |
1.526 |
2 |
1.524 |
3 |
1.524 |
Mean ± SD |
1.525±0.001 |
SD = Standard deviation
Calibration Data
Retention time data of the reference items used for calibration of soil and sewage sludge are given in the tables below.
Capacity Factors (k’)of the Reference Items for Soil and Sewage Sludge
Reference item |
logKOC,soil |
logKOC,sewage sludge |
1. Calibration |
2. Calibration |
||||
tR1 |
k' |
logk' |
tR2 |
k' |
logk' |
|||
Acetanilide |
1.26 |
1.52 |
3.655 |
1.40 |
0.145 |
3.655 |
1.40 |
0.145 |
Atrazin |
1.81 |
1.66 |
4.591 |
2.01 |
0.303 |
4.592 |
2.01 |
0.304 |
Aniline |
2.07 |
1.74 |
4.679 |
2.07 |
0.316 |
4.438 |
1.91 |
0.281 |
2,5-Dichloroaniline |
2.55 |
2.45 |
6.547 |
3.29 |
0.518 |
6.527 |
3.28 |
0.516 |
Linuron |
2.59 |
2.43 |
8.915 |
4.85 |
0.685 |
8.891 |
4.83 |
0.684 |
Phenyl benzoate |
2.87 |
3.26 |
8.908 |
4.84 |
0.685 |
8.880 |
4.82 |
0.683 |
Fenthion |
3.31 |
3.75 |
17.544 |
10.5 |
1.02 |
17.517 |
10.5 |
1.02 |
tR1 =
Retention time 1st injection tR2 =
Retention time 2nd injection
k’ =
Capacity factor KOC =
Adsorption coefficient
The corresponding calibration data for the corresponding calibration lines are summarized inTable 7. The calibration data were obtained from the regression line of the graph log KOC vs. logk’ .
Calibration Data of the Reference Items for Soil and Sewage Sludge
|
Soil |
Sewage Sludge |
||
|
1. Calibration |
2. Calibration |
1. Calibration |
2. Calibration |
slope |
2.226 |
2.182 |
2.711 |
2.679 |
intercept |
1.184 |
1.219 |
0.979 |
1.011 |
r2 |
0.9287 |
0.9163 |
0.9075 |
0.9092 |
r |
0.9637 |
0.9572 |
0.9526 |
0.9535 |
r : Correlation coefficient r2: Coefficient of determination
Results of Test Item Analysis
The table below contains the retention time data and calculated log k´ values for N,N-Dimethylnonanamide.
Two individual logKOC values for the test item were derived under consideration of the first and second calibration. Finally, a mean value was calculated from these individual logKOC value for soil and for sewage sludge.
Retention Times, Capacity Factors (k’) and
Adsorption Coefficient (logKOC)of
N,N-Dimethylnonanamidefor Soiland Sewage Sludge
|
N,N-Dimethylnonanamide |
||||||
tR |
k’ |
logk’ |
logKOC, soil |
logKOC,sewage sludge |
|||
1stCalibration |
2ndCalibration |
1stCalibration |
2ndCalibration |
||||
6.399 |
3.20 |
0.505 |
2.31 ± 0.08 (95% CI) |
2.32 ± 0.08 (95% CI) |
2.35 ± 0.11 (95% CI) |
2.36 ± 0.11 (95% CI) |
|
6.391 |
3.19 |
0.504 |
|||||
6.391 |
3.19 |
0.504 |
|||||
Mean |
6.394 |
3.19 |
0.504 |
2.32 |
2.35 |
tR= Retention time SD = Standard deviation CI = Confidence interval
Description of key information
The adsorption coefficient log KOC of the test item N,N-Dimethylnonanamide was determined to be 2.32 for soil and 2.35 for sewage sludge.
Key value for chemical safety assessment
- Koc at 20 °C:
- 2.32
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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