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Diss Factsheets
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EC number: 277-539-5 | CAS number: 73570-52-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May/Jun 1982
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1)
- EC Number:
- 264-355-5
- EC Name:
- 3,7-bis(diethylamino)phenoxazin-5-ium tetrachlorozincate (2:1)
- Cas Number:
- 63589-47-9
- Molecular formula:
- C20H26N3O.1/2Cl4Zn
- IUPAC Name:
- bis[3,7-bis(diethylamino)phenoxazin-5-ium] tetrachlorozincate(2-)
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: ca. 9 wks (male), ca. 14 wks (female)
- Weight at study initiation: 157 - 189 g (male), 152 - 174 g (female)
- Fasting period before study: 4 - 16 h
- Housing: Makrolon III cage, 5 animals per cage
- Diet (e.g. ad libitum): ad lib.
- Water (e.g. ad libitum): ad lib.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20.5 - 23.5°C
- Humidity (%): 55 - 65% rh
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Remarks:
- tap water
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 20 mL/kg
- Justification for choice of vehicle: water soluble
MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg - Doses:
- 63, 100, 200, 225, 250, 310 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations daily, weighing on day 0, 7 and 14
- Necropsy of survivors performed: yes, randomly (not all animals)
- Other examinations performed: clinical signs, body weight - Statistics:
- Probit analysis
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 130 mg/kg bw
- Based on:
- act. ingr.
- 95% CL:
- 114 - 140
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 217 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 190 - < 234
- Mortality:
- high dose: 100%
2 lowest doses: 0% - Clinical signs:
- in all concentrations except low dose (from 1 min up to 2 d after dosing): bad general condition, sedation, prone and lateral position, nausea, breathing difficulties, no difference in sexes
- Body weight:
- no effects
- Gross pathology:
- two highest doses: no pathology of stomach/intestine possible due to heavy staining and discolouration, other organs w/o effects
mid doses: slight gastritis
low dose: no effects
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- The substance was admintered orally (gavage) in male and female rats. The LD50 was determined at 130 mg/kg bw (act. ingr.). Therefore, the source substance is classified as Category 3 for acute oral toxicity. The 95% CI interval is within the range of GHS Category 3, too.
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