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EC number: 251-090-5 | CAS number: 32539-83-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Between 1999-10-19 and 1999-10-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to 92/69/EEC C.2 and OECD test guideline 202
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,4,5,6,7,8,9,10,11,12,13,14-dodecahydro-2H-cyclododeca[b]pyran
- EC Number:
- 251-090-5
- EC Name:
- 3,4,5,6,7,8,9,10,11,12,13,14-dodecahydro-2H-cyclododeca[b]pyran
- Cas Number:
- 32539-83-6
- Molecular formula:
- C15H26O1
- IUPAC Name:
- 2H,3H,4H,5H,6H,7H,8H,9H,10H,11H,12H,13H,14H-cyclododeca[b]pyran
- Details on test material:
- - Name of test material (as cited in study report): DDP
- Substance type: active substance
- Physical state: viscous liquid
- Isomers composition: no isomers
- Purity test date: 1999.02.17
- Lot/batch No.: 9020015
- Expiration date of the lot/batch: 2000.07
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Analysis of all test concentrations plus a control at test start and after 48h of exposure (in the nominal concentration 0.25 mg/L, after 24h of exposure because of early 100% immobilization rates)
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUS, parthenogenetic females
- Source: cloned at the Federal Health Office (BGA) in Berlin
- Age at study initiation (mean and range, SD): 1 - 24hrs
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 24 h
- Post exposure observation period:
- 24 hours
Test conditions
- Hardness:
- 15.5 °dH (M4-Medium)
- Test temperature:
- 20.0 - 20.1°C, at 48h
- pH:
- 87.9 - 8.9 at 48h
- Dissolved oxygen:
- 8.2 - 8.7 mg/L at 48h
- Nominal and measured concentrations:
- 0 hours, nominal vs measured concentrations in mg/L : control < 0.01, 0.03 vs<0.018, 0.06 vs 0.037, 0.13 vs 0.10, 0.25 vs 0.22
48 hours: nominal vs measured concentrations in mg/L: control < 0.01, 0.03 vs<0.013, 0.06 vs 0.030, 0.13 vs 0.061, 0.25 vs 0.15 - Details on test conditions:
- TEST SYSTEM
- Test vessel: "Karlsruher bottles"
- Material, size, headspace, fill volume: bottles with stoppers, 250-300 ml test medium, headspace
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Solubilizer or other auxiliaries: ultra-turrax magnetic stirrer folded filter
Control: M4- Medium according to Elendt and BGA (1992)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4- Medium according to Elendt and BGA (1992), used also as control
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours light/ 8hours dark - Reference substance (positive control):
- not required
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 0.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- ca. 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 0.02 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- ca. 0.08 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Reported statistics and error estimates:
- Geometric mean after 24 hours: EC0/EC100: 0.12 mg/L
Geometric mean after 48 hours: EC0/EC100: 0.04 mg/L
Any other information on results incl. tables
Immobile daphnids absolute % | Oxygen | pH | Temperature | ||||
Nominal Concentration test substance (mg/l) | 24h | 48h | 24h | 48h | 48 h (mg/L) | 48h | 48 h (°C) |
control | 0 | 0 | 0 | 0 | 8.7 | 8.0 | 20.1 |
0.03 | 0 | 0 | 0 | 0 | 8.6 | 8.0 | 20.0 |
0.06 | 0 | 5 | 0 | 25 | 8.2 | 7.9 | 20 .0 |
0.13 | 18 | 20 | 90 | 100 | 8.3 | 7.9 | 20.1 |
0.25 | 20 | 20 | 100 | 100 | 8.5 * | 7.9 * | 20.3 * |
To accelerate the solution procedure a thirty fold amount of the maximum water solubility under exposure conditions was taken (10 mg/l). To produce the stock solution the test substance was weighed into water, treated for 60 seconds at 8000 rpm. with an ultra-turrax and afterwards stirred for 24 hours on a magnetic stirrer. To remove undissolved particles, the resulting suspension was filtered using a folded filter of the pore size 7-12 microm. Deviating from the above test guideline and considering the decrease of the test concentrations during the preliminary experiment, the main test was conducted under closed-bottle conditions in so-called "Karlsruher bottles".
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control , 0% of the daphnids was immobilised (<10% required in the guidance). 2.The dissolved oxygen concentration at the end of the test is 8 mg/l in control and test vessels (>=32 mg/L required in the guidance)
- Conclusions:
- EC50 at 48 hours for the test material determined by geometric mean is 0.04 mg/l.
- Executive summary:
The test material has been tested following the procedure "Acute Toxicity for Daphnia" of 92/69/EEC C.2. Four defined concentrations of the test material plus a control will lead to a certain percentage proportion of immobile daphnids at the end of the study period. The EC0 and EC100 have been determined directly from the study, while the EC50 has been calculated by the geometric average. The study lasted for 48hours. The test material has a EC50 of 0.04 mg/l. The study report fulfill sthe criteria of validity of the followed procedure.
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