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EC number: 204-844-2 | CAS number: 127-47-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-02-01 - 1993-11-06
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: According to OECD guideline 203
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Principles of method if other than guideline:
- not relevant
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Retinyl acetate
- EC Number:
- 204-844-2
- EC Name:
- Retinyl acetate
- Cas Number:
- 127-47-9
- Molecular formula:
- C22H32O2
- IUPAC Name:
- (2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraen-1-yl acetate
- Details on test material:
- - Name of test material (as cited in study report): Vitamin A-Acetat
- Physical state: yellow solid
- Storage condition of test material: room temperature, in dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling method: Taken from the center of the test vessels immediately before exposure and after 96 h
- Sample storage conditions before analysis: kept at -18 to -22 degrees C
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 4.00 g test substance were mixed with 4.00 g of a mixture 1-methyk-2-pyrrolydone (NMP) / polyoxy-ethylene-sorbitanmonoleate
(TWEEN80) (96%/4%)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): 1-methyk-2-pyrrolydone (NMP) / polyoxy-ethylene-sorbitanmonoleate
(TWEEN80) (96%/4%)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): deposit of test substance was observed in the test vessel at all test times and
test concentrations.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow Trout
- Source: P. Hohler/CH-4314 Zeininge
- Length at study initiation (length definition, mean, range and SD): 50 mm (37-55 mm)
- Weight at study initiation (mean and range, SD): 1.05 g (0.47-1.30 g)
- Feeding during test: no
ACCLIMATION
- Acclimation period: 12 days
- Feeding frequency: 24 h, no food 24 h prior to exposure
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no data
Test conditions
- Hardness:
- 183 CaCo3/l
- Test temperature:
- 14 ± 1 degrees C
- pH:
- 7.8-8.2
- Dissolved oxygen:
- 70-105% at t=0h to 92-104% at t=96h
- Salinity:
- not relevant
- Nominal and measured concentrations:
- nominal concentrations: 0, 10, 18, 32, 58 and 100 mg/L
measured concentration (start): <0.1, 0.1, 0.3, 1.7, 4.0, 15.6
measured concentration at 96h: <0.1 in all exposures - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass aquaria of 20 L filled with 15 L (36.22.25 cm)
- Aeration: gentle aeration during exposure
- No. of organisms per vessel: 10
- Biomass loading rate: 0.70 g/L
OTHER TEST CONDITIONS
- Photoperiod:16 h
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.37 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: nominal concentration calculated 43 mg/L, 95%CL 36-51 mg/L
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.37 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: nominal concentration calculated 43 mg/L, 95%CL 36-51 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.37 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: nominal concentration calculated 43 mg/L, 95%CL 36-51 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.875 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: nominal concentration calculated 32 mg/L, 95%CL 65-89 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.875 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: nominal 32 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 2.03 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: nominal 58 mg/L
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.38 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: nominal concentration calculated 76 mg/L, CL% 65-89 mg/L
- Details on results:
- - Behavioural abnormalities: Moderate symptoms were disorders in swimming behaviour and respiratory function, loss of equilibrium and severe pigmentation in the 58 mg/L exposure group
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:not relevant
- Effect concentrations exceeding solubility of substance in test medium: not relevant because substance was solubilized - Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- Calculated according to Berkson, JASA 48 (1953), 569-599
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Test substance is instable in water and was degraded by light and therefore was lost during the test. Due to the fact that a clear dose-effect response was observed the LC50 was cacluated based on average measured concentrations
Applicant's summary and conclusion
- Conclusions:
- The 96h LC50 of Vitamin A acetate to Rainbow trout was found to be 1.37 mg/l, the 96h NOEC was found to be 0.875 mg/l and the 96 h LC100 was 2.03 mg/L.
- Executive summary:
The toxicity of Vitamin A acetate towards Rainbow trout was determined according to OECD Guideline 203 under GLP conditions. Exposure concentrations decreased during exposure period, therefore concentrations are expressed on average measured concentrations. All other validity criteria were met. The 96h LC50 of Vitamin A acetate to Rainbow trout was found to be 1.37 mg/l, the 96h NOEC was found to be 0.875 mg/l and the 96 h LC100 was 2.03 mg/L.
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