3,4-diethoxyphenylacetic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,4-diethoxyphenylacetic acid

EC Number:

253-957-3

EC Name:

3,4-diethoxyphenylacetic acid

Cas Number:

38464-04-9

Molecular formula:

C12H16O4

IUPAC Name:

2-(3,4-diethoxyphenyl)acetic acid

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Aerobic activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage treatment plant Bensheim, Germany

Duration of test (contact time):

>= 28 d

Initial test substance concentration

Initial conc.:

ca. 0.104 g/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The test flasks prepared according paragraph 6.6 were incubated at 22°C ± 1°C. The pressure decrease in the reaction vessels was measured over complete experimental phase of 28 days using the BSB/BODSensor-
System, Aqualytic Dortmund, Germany. The test flasks were closed gas-tight by a measuring head. Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated.
from the decrease of pressure in the reaction vessel.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 0

Sampling time:

28 d

Details on results:

The degradation rate of DIETHOXY ACID was 0% and after 28 days (THODNH4).
The reference item sodium benzoate was sufficiently degraded to 87% after 14 days and to 89% after 28 days of incubation.

BOD5 / COD results

BOD5 / COD

Key result

Parameter:

ThOD

Value:

0 mg O2/g test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The substance is not biodegradable after 28 days, in this test condition