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EC number: 205-778-7 | CAS number: 150-90-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Toxicity of test material to Bacteria Klebsiella pneumoniae H12 was evaluated in the study for the duration of 96 hr.The toxicity is evaluated based on Effect concentration observed.
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Details on sampling:
- 405.13,810.26,1620.52,3241.04,6482.08, 12964.16mg/l
- Vehicle:
- not specified
- Details on test solutions:
- No data available
- Test organisms (species):
- other: Klebsiella pneumoniae H12
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 4 d
- Hardness:
- No data available
- Test temperature:
- 28⁰ C
- pH:
- 7.0
- Salinity:
- No data available
- Nominal and measured concentrations:
- 405.13,810.26,1620.52,3241.04,6482.08, 12964.16mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open / closed No data available
- Material, size, headspace, fill volume: No data available
- Aeration: Yes, with a totary shaker (250 rpm with a 50-mm stroke)
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: No data available
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control (replicates): No data available
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available
OTHER TEST CONDITIONS
- Adjustment of pH: Yes, pH was adjusted to 7.0
- Photoperiod: No data available
- Light intensity: No data available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Polysaccharide productivity
TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study No data available
- Test concentrations: 2.5, 5.0, 10.0, 20.0, 40.0, 80.0 mM (4051.3, 8102.6, 16205.2, 32410.4, 64820.8, 129641.6 mg/L)
- Results used to determine the conditions for the definitive study: No data available - Duration:
- 4 d
- Dose descriptor:
- other: EC
- Effect conc.:
- 3 241.04 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Decrease in the polysaccharide productivity
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
- Conclusions:
- The EC for the test material was found to be 20mM (3241.04 mg/L).
- Executive summary:
Klebsiella pneumonieH12 is a polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, , two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using test material. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and test material was studied. In an assay to study polysaccharide productivity, Strain H12 was cultured for 4 days at 28°C in 250-ml Erlenmeyer flasks containing 30 ml of YPE medium. Production of the polysaccharide was measured by the dry weight after ethanol precipitation of the culture supernatant. Polysaccharide production was promoted at low concentration of upto 10mM but the productivity decreased from 20mM ( 3241.04 mg/L) dose range. The EC for the test material is thus found to be 20mM ( 3241.04 mg/L).
Reference
Description of key information
Toxicity to microorganism:
Klebsiella pneumonieH12 is a polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, , two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using test material. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and test material was studied. In an assay to study polysaccharide productivity, Strain H12 was cultured for 4 days at 28°C in 250-ml Erlenmeyer flasks containing 30 ml of YPE medium. Production of the polysaccharide was measured by the dry weight after ethanol precipitation of the culture supernatant. Polysaccharide production was promoted at low concentration of upto 10mM but the productivity decreased from 20mM ( 3241.04 mg/L) dose range. The EC for the test material is thus found to be 20mM ( 3241.04 mg/L).
Key value for chemical safety assessment
Additional information
Toxicity to microorganism:
Effect of test material was evaluated on microorganisms based on the data of target chemical from peer reviewed journal.
Klebsiella pneumonieH12 is a polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, , two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using test material. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and test material was studied. In an assay to study polysaccharide productivity, Strain H12 was cultured for 4 days at 28°C in 250-ml Erlenmeyer flasks containing 30 ml of YPE medium. Production of the polysaccharide was measured by the dry weight after ethanol precipitation of the culture supernatant. Polysaccharide production was promoted at low concentration of upto 10mM but the productivity decreased from 20mM ( 3241.04 mg/L) dose range. The EC for the test material is thus found to be 20mM ( 3241.04 mg/L).
In another study , Klebsiella pneumonieH12 is a bacteria and polysaccharide flocculant that is involved to mediate plant-microbe interaction. In the given study, two- way regulation of polysaccharide-mediated interactions betweenA. pneumoniasandRaphanus sativuswas studied using sodium succinate. Polysaccharide productivity and flocculation activity assayed using kaolin, polysaccharide and title comp was studied. In the flocculation assay -in a test tube, 9 ml of a kaolin clay suspension (5.5 g /l) was mixed with 1 ml of polysaccharide solution, 100 µl of or- ganic acid solution, and 100 µl CaCl2.2H2O solution in this sequence with a Vortex mixer for 5 sec. After 5 min, OD550c of the supernatant was measured. A control experiment was done in the absence of the polysaccharide and the OD550cwas measured. Flocculation of bacterial cells by polysaccharide was inhibited at a concentration of 0.3mM (48.61 mg/l) by the test compound .The EC for the test material Sodium succinate is thus found to be 0.3mM (48.61 mg/l).
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