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EC number: 700-345-3 | CAS number: 515851-08-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- , The following deviation from the study plan was documented: -The temperature in the main study was higher than stated in the guidelines. As algal growth in the controls was normal, this deviation was stated as uncritical. The deviation was signed and a
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- , The composition of stock solution ll is taken from OECD Guideline 201. As in EU-Method C.3, a different composition (80 mg FeCl3*6HzO/L) is stated, this is stated as a deviation from the EU Method.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [TN]Kn 172[/TN][SPEC][/SPEC][AM]96.2%[/AM]
- IUPAC Name:
- [TN]Kn 172[/TN][SPEC][/SPEC][AM]96.2%[/AM]
- Reference substance name:
- N4-[3-(1H-imidazol-1-yl)propyl]-2-methylbenzene-1,4-diamine trihydrochloride
- EC Number:
- 700-345-3
- Cas Number:
- 515851-08-8
- Molecular formula:
- C13H21Cl3N4
- IUPAC Name:
- N4-[3-(1H-imidazol-1-yl)propyl]-2-methylbenzene-1,4-diamine trihydrochloride
- Details on test material:
- Name: Kn 172
Batch no.: Kn-Gi 9328-182
Appearance: white powder
Molecular formula: C13H18N4 x 3HCl
Molecular weight: 339.69 g/mol
Purity: NMR: 96.2%, 0.8% EtOH, 1.4% water
Homogeneity: not stated
Vapour pressure: not stated
Stability: unknown
Solubility H2O: > 1 g/L; EtOH; acetone; CH3CN; DMSO: unknown
Production date: not stated
Expiry date: 10. Jun. 2013
Storage: fridge 2 - 8° C, keep away from light and humidity, keep under Ar!
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 8.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 5.6 - 14.9 mg/L
- Duration:
- 72 h
- Dose descriptor:
- other: EbC50
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: AUC (Area Under Curve according to EU Method C.3)
- Remarks on result:
- other: 1.4 - 3.4 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Kn 172 is considered toxic to algae (Desmodesmus subspicatus)
- Executive summary:
Two experiments were performed.
As the test item wasn't stable under test conditions and in the samples which were taken
for analytical determinations, the results of the analytical determinations in the first experiment
were considered as insufficient. Therefore, a second experiment was performed.
The study was performed using five concentrations ranging from 1.0 to 22 mg/L. The
treatments were used to incubate the unicellular freshwater green algae Desmodesmus
subspicatus for a period of 72 hours. The cell concentration of each replicate was determined
microscopically with counting chamber every 24 hours. From the measured cell
numbers, the growth rate µ, the area under the growth curve (AUC) and the Yield were
determined.
At the start, after 2, 24 and 48 hours, the content of the test item in the test solutions was
determined using HPLC. All samples are measured separately and directly after sampling
and not by using an auto sampler.
The measured concentration at the beginning of the test was between 82 % and 104 % of
the nominal concentration. The recovery after 2 hours was in a range of 56 and 69 % of
the start concentration. After 24 hours, no test item was detectable. Therefore, calculation
of the mean of the measured concentration at the start and the end of the test wasn't feasible.
lt is believed that the toxic effects of the test item took part within the first 24 hours.
The determination of the results was based on the nominal concentrations.
The EC50s of potassium dichromate were tested in a current reference test. The values
lay within the normal range of the laboratory.
The following results for the test item Kn 172 were determined:
Endpoint NOEC LOEC EC50 Growth Rate 2.2 mg/L 4.6 mg/L 8.3 mg/L AUC 1.0 mg/L 2.2 mg/L 2.3 mg/L Yield 2.2 mg/L 4.6 mg/L 2.4 mg/L
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