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EC number: 942-520-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July to August 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants, Growth Test)
- Version / remarks:
- adopted July 19, 2006
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): The test concentration of 1000 mg/kg dw was prepared for two pieces of 4750 g. 4.7503 and 4.7501 g test item were weighed and mixed with 50 g soil, respectively.These mixtures was mixed with 4700 g dry soild each to be homogenous with the blender to get 1000 mg/kg dw. For the main test with pea 5500 g soil mixtures of the concentration of 100, 211, 447, 946 and 2000 mg/kg/kg were prepared of 0.5503 g, 1.1607g, 2.4610 g5.2031 g and 11.0078 g test item with 50g and afterwards 5450 g dry soil.
- Controls: The blank control group was maintained under identical conditions in parallel, only without test application. - Species:
- Triticum aestivum
- Plant group:
- other: Poaceae (Gramineae)
- Details on test organisms:
- Origin: Shenyang Agricultural University Seed Industry
- Species:
- Cucumis sativus
- Plant group:
- other: Cucurbitaceae
- Details on test organisms:
- Origin: Heiongjiang Harbin Seed Breeding Center
- Species:
- Pisum sativum
- Plant group:
- other: Fabaceae (Leguminosae)
- Details on test organisms:
- Origin: Shenyang Gaotian Seed Industry Co. Ltd.
- Test type:
- seedling emergence and seedling growth test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- yes
- Total exposure duration:
- 16 d
- Remarks:
- For pea no limit test was performed.
- Test temperature:
- 24-27 °C
- pH:
- 6.57
- Moisture:
- Humidity: 68-87%
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): plastic pot with diameter of 15 cm and depth of 10 cm
- Amount of soil: 600 g (dry weight)
- No. of seeds per container: 5 (wheat), 2 (cucumber), 3 (pea)
- No. of replicates per treatment group: 4 (wheat), 4 (pea, 2 containers per replicate), 5 (cucumber, 2 containers per replicate)
- No. of replicates per control: 4 (wheat and pea), 5 (cucumber)
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Farmland Gongzhuling County of Changchun City, Jilin Province, not cultivated
- Pesticide use history at the collection site: no history of pesticide and fertlizer application for 1 year
- Soil texture (if natural soil)
- % sand: 30.0 %
- % silt: 44.8 %
- % clay: 25.2 %
- Organic carbon (%): 1.72 %
- Soil moisture content at start of the test: 1.42 %
- Storage (condition, duration): 2-8 °C
GROWTH CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity and quality: 18100 Lux- 20100 Lux, cool white flourescen
- Relative humidity (%): 68-87 %
- Water source/type: tap water
- Interval of applications: daily
- Method of application: Water was absorbed from the bottom (tap water was added to the tray) after the seeds have been planted
RANGE FINDING STUDY
- Test concentrations: 10,100,1000 mg/kg dw
- Results used to determine the conditions for the definitive study:
- Wheat: 1.7 %, 4.9 %, 4.1 % inhibition
- Cucumber: 8.1 %, 8.7, 6.8 % inhibition
- Pea: 6.3 %, 12.3 %, 46.3 % inhibition - Nominal and measured concentrations:
- Wheat and cucumber: 1000 mg/kg dw
Pea: 100, 211, 447, 946 and 2000 mg/kg dw - Reference substance (positive control):
- no
- Key result
- Species:
- Triticum aestivum
- Duration:
- 16 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Key result
- Species:
- Triticum aestivum
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Key result
- Species:
- Cucumis sativus
- Duration:
- 16 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Key result
- Species:
- Cucumis sativus
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Key result
- Species:
- Pisum sativum
- Duration:
- 16 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Key result
- Species:
- Pisum sativum
- Duration:
- 16 d
- Dose descriptor:
- EC50
- Effect conc.:
- 2 089 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks on result:
- other: EC50 = 1030-4238 mg/kg dw
- Details on results:
- Measured concentrations at beginning of the test were 86.1 - 94.6% of nominal concentrations.
No visible phototoxic effects (e. g. chlorosis, necrosis, wilting, leaf and stem deformations). Plants exhibited only normal variation in growth and morphology for each particular species. - Reported statistics and error estimates:
- The result of the Two-sided Student t-test indicated that the emergence of three species did not display significant differences (p> 0.05) between the control and each test group.
For wheat and cucumber, the dry shoot weight did not display significant differences (p> 0.05) between the control and the test group of 1000 mg/kg dw.
For pea, the dry shoot weight showed a visible concentration-dependent decrease. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this test the test substance did not show terrestrial toxicity on the three species Wheat (Triticum aestivum), Cucumber (Cucumis sativus) and Pea (Pisum sativum).
- Executive summary:
A terrestrial plant test with Guerbet alcohols, C24 -26, branched and cyclic was conducted with three plant species (wheat, cucumber and pea) according to OECD Guideline 208. For wheat and cucumber a limit test was performed with 1000 mg/kg dw. For pea the test was carried out with the following test concentrations: 100, 211, 447, 946 and 1000 mg/kg dw.
For the three plant species the seedlings didn't exhibit visible phytotoxic effetcs and the plants exhibited only normal variation in growth and morphology for that particular species.
For wheat and cucumber the dry shoot weight did not display significant differences (p> 0.05) between the control and the test groups of 1000 mg/kg dw. For pea a dose-related decrease of dry shoot weight was observed. According to the test results the effective concentrations of the test substance to the three terrestrial plants was: NOEC (germination) > 1000 mg/kg dw and EC50 (dry shoot weight) > 1000 mg/kg dw.
Reference
Table 1: Germination, survival plants and plant dry weight for Wheat
Concentration | Mean germination rate | Inhibition rate of germination | p-value (t-test) | Mean survival rate | inhibition rate of survival |
Average dry weight per shoot (g) |
Inhibition rate to weight (%) |
Control |
95 |
- |
- |
100 |
- |
0.0854 |
- |
1000 mg/kg dw |
100 |
- 5.3 |
0.36 |
100 |
0.0 |
0.0963 |
-11.6 |
Table 2: Germination, survival plants and plant dry weight for Cucumber
Concentration | Mean germination rate | Inhibition rate of germination | p-value (t-test) | Mean survival rate | inhibition rate of survival |
Average dry weight per shoot (g) |
Inhibition rate to weight (%) |
Control |
95 |
- |
- |
100 |
- |
0.2810 |
- |
1000 mg/kg dw |
100 |
5.0 |
0.35 |
100 |
0.0 |
0.2578 |
8.3 |
Table 3: Germination, survival plants and plant dry weight for Pea
Concentration | Mean germination rate | Inhibition rate of germination | p-value (t-test) | Mean survival rate | inhibition rate of survival |
Average dry weight per shoot (g) |
Inhibition rate to weight (%) |
Control |
87 |
- |
- |
100 |
- |
0.1599 |
- |
100 mg/kg dw |
83 |
4.6 |
0.62 |
100 |
0.0 |
0.1711 |
-7.0 |
211 mg/kg dw |
79 |
9.2 |
0.21 |
100 |
0.0 | 0.1565 |
2.1 |
447 mg/kg dw |
75 |
13.8 |
0.10 |
100 |
0.0 |
0.1366 |
14.6 |
946 mg/kg dw |
79 |
9.2 |
0.21 |
100 |
0.0 |
0.1169 |
26.9 |
2000 mg/kg dw |
75 |
13.8 |
0.1 |
100 |
0.0 | 0.0914 |
42.8 |
Description of key information
A terrestrial plant test with Guerbet alcohols, C24 -26, branched and cyclic was conducted with three plant species (wheat, cucumber and pea) according to OECD Guideline 208. For Wheat and cucumber a limit test was performed with 1000 mg/kg dw. For pea the test was carried out with the following test concentrations: 100, 211, 447, 946 and 1000 mg/kg dw.
For the three plant species the seedlings didn't exhibit visible phytotoxic effetcs and the plants exhibited normal variation in growth and morphology for that particular species.
For wheat and cucumber the dry shoot weight did not display siginifcant differences (p> 0.05) between the control and the test groups of 1000 mg/kg dw. For pea a dose-related decrease of dry shoot weight was observed. According to the test results the effective concentrations of the test substance to the three terrestrial plants was: NOEC (germination) > 1000 mg/kg dw and EC50 (dry shoot weight) > 1000 mg/kg dw.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for terrestrial plants:
- 1 000 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 1 000 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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