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EC number: 217-006-6 | CAS number: 1719-57-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Chloro(chloromethyl)dimethylsilane
- EC Number:
- 217-006-6
- EC Name:
- Chloro(chloromethyl)dimethylsilane
- Cas Number:
- 1719-57-9
- Molecular formula:
- C3H8Cl2Si
- IUPAC Name:
- chloro(chloromethyl)dimethylsilane
- Test material form:
- other: colourless liquid
- Details on test material:
- Purity 99.4% (GC)
Constituent 1
Method
- Target gene:
- his D 3052
his G 46
his G 428
his C 3076
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- Post-mitochondrial fraction (59 fraction) from rats treated with Aroclor 1254 was prepared according to MARON and AME5 (1983). 59 was collected from 20 - 30 rats.
- Test concentrations with justification for top dose:
- 100, 316, 1000, 3160 and 5000 ug/plate
3 plates per concentration and experiment - Vehicle / solvent:
- ethylene glycol dimethylether
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- cyclophosphamide
- methylmethanesulfonate
- other: 2-anthracene amide
- Details on test system and experimental conditions:
- Ethylene glycol dimethylether served as solvent for the test substance and as negative control in all test strains.
- Evaluation criteria:
- The statistical evaluation of the results of the AMES test is still under discussion
A test chemical is considered to show a positive response if
the number of revertants is significantly increased (p <= 0.05, U-test according to
MANN and WHITNEY, compared with the solvent control to at
least 2-fold of the solvent control for TA 98, TA 100 and TA 102 and 3-fold of
the solvent control for TA 1535 and TA 1537 in both independent experiments;
in addition, a significant (p <= 0.05) concentration (log value)-related effect
(Spearman's rank correlation coefficient, see reference 3.) is observed;
positive results have to be reproducible and the histidine independence of the
revertants has to be confirmed by streaking random samples on histidine-free
agar plates. - Statistics:
- The statistical evaluation of the results of the AMES test is still under discussion
A test chemical is considered to show a positive response if
the number of revertants is significantly increased (p <= 0.05, U-test according to
MANN and WHITNEY, compared with the solvent control to at
least 2-fold of the solvent control for TA 98, TA 100 and TA 102 and 3-fold of
the solvent control for TA 1535 and TA 1537 in both independent experiments;
in addition, a significant (p <= 0.05) concentration (log value)-related effect
(Spearman's rank correlation coefficient, see reference 3.) is observed;
positive results have to be reproducible and the histidine independence of the
revertants has to be confirmed by streaking random samples on histidine-free
agar plates.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- No precipitation of the test substance occurred up to the highest investigated dose.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Two independent experiments were carried out each without and with metabolic
activation, each experiment consisted of 3 plates/concentration.
The first experiment was carried out by the standard plate incorporation method
whereas the second was carried out by the preincubation method. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In conclusion, under the present test conditions Chlormethyl-dimethyl-chlorsilan tested
up to 5000 ug/plate caused no mutagenic effect in the Salmonella typhimurium strains
TA 98, TA 100, TA 102, TA 1535 and TA 1537 neither in the plate incorporation test
nor in the preincubation test each carried out without and with metabolic activation - Executive summary:
Chlormethyl-dimethyl-chlorsilan was examined in the 5 Salmonella typhimurium strains
TA 98, TA 100, TA 102, TA 1535 and TA 1537 in two independent experiments, each
carried out without and with metabolic activation (a microsomal preparation derived
from Aroclor 1254-induced rat liver). The first experiment was carried out as a plate
incorporation test and the second as a preincubation test.
Preliminary test
Chlormethyl-dimethyl-chlorsilan was examined in a preliminary cytotoxicity test without
metabolic activation in test strain TA 100. Ten concentrations of Chlormethyldimethyl-
chlorsilan ranging from 0.316 to 5000 ug/plate were examined in the
preliminary test using strain TA 100. Slight cytotoxicity (scarce background lawn) was
noted at the top concentration of 5000 ug/plate. Hence, 5000 ug/plate was chosen as
the top concentration for the main study.
Main study
Five concentrations ranging from 100 to 5000 ug Chlormethyl-dimethylchlorsilan/
plate were employed in two independent experiments each carried out
without and with metabolic activation.
Cytotoxicity
In the plate incorporation test without and with metabolic activation slight cytotoxicity
(scarce background lawn) was noted at the top concentration of 5000 ug Chlormethyldimethyl-
chlorsilan/plate in all test strains.
In the preincubation test without and with metabolic activation pronounced cytotoxicity
(reduction of the number of revertants by more than 50%) was noted at the top
concentration of 5000 ug Chlormethyl-dimethyl-chlorsilan/plate in all test strains and at
3160 ug/plate in test strain TA 102. Scarce background lawn was observed at the
concentrations of 31 60 and 5000 ug Chlormethyl-dimethyl-chlorsil.anlplate in all test
strains.
Mutagenicity
No mutagenic effect (no increase in revertant colony numbers as compared with control
counts) was observed for Chlormethyl-dimethyl-chlorsilan tested up to a concentration
of 5000 ug/plate in any of the 5 test strains in two independent experiments without
and with metabolic activation (plate incorporation or preincubation test).
In conclusion, under the present test conditions Chlormethyl-dimethyl-chlorsilan tested
up to 5000 ug/plate caused no mutagenic effect in the Salmonella typhimurium strains
TA 98, TA 100, TA 102, TA 1535 and TA 1537 neither in the plate incorporation test
nor in the preincubation test each carried out without and with metabolic activation
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