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EC number: 203-309-0 | CAS number: 105-56-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethyl cyanoacetate
- EC Number:
- 203-309-0
- EC Name:
- Ethyl cyanoacetate
- Cas Number:
- 105-56-6
- Molecular formula:
- C5H7NO2
- IUPAC Name:
- ethyl 2-cyanoacetate
- Details on test material:
- Batch No. T 434
ID No. 3633/81 410
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Himalayan
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- 0.1 cm³ undiluted test material
- Duration of treatment / exposure:
- 24 hours
- Observation period (in vivo):
- 1, 24, 48, 72 hours, 6 and 8 days
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing: with warm isotonic solution of sodium chloride
- Time after start of exposure: 24 hours
SCORING SYSTEM:cornea score, conjunctivae score, iris score, chemosis score
TOOL USED TO ASSESS SCORE: fluorescein
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 24 - 72 h
- Score:
- 1.22
- Reversibility:
- fully reversible within: 8 days
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- other: 24 - 72 h
- Score:
- 0.78
- Reversibility:
- fully reversible within: 8 days
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 24 - 72 h
- Score:
- 0.11
- Reversibility:
- fully reversible within: 8 days
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24 - 72 h
- Score:
- 0.78
- Reversibility:
- fully reversible within: 8 days
Any other information on results incl. tables
Individuell results
1h | 24h | 48h | 72h | 6d | 8d | |||||||||||||
animal | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 |
conjunctivae score | 2 | 1 | 2 | 2 | 1 | 1 | 2 | 2 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | |
chemosis score | 1 | 1 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | |
iris score | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
cornea score | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | |
fluorescein test* | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | ||||||
exudation | x | x | x | x | ||||||||||||||
- of substance | ||||||||||||||||||
- white mucous | x | x | x | x | ||||||||||||||
conjunctivae, incl. nictitating membrane- with hemorrhage | ||||||||||||||||||
iris | ||||||||||||||||||
- reddened | x | |||||||||||||||||
- circumcomeal injection |
* the area of the brightening from the cornea surface after instillation of one trop fluorescein (0,1%) is denoted:
0 = no brightening
1 = punctual to 1/4
2 = 1/4 to 1/2
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Classification: irritating
The following mean scores were calculated from the individual examinations performed 24 h, 48 h and 72 h p.a.:
Cornea score: 0.78
Conjunctivae score: 1.22
Iris score: 0.11
Chemosis score: 0.78
Ocular lesions were reversible in all animals. A clearly hyperemia respectively a redness and a light swelling of the conjunctivae was observed in all
animals after one hour exposition.
Mean scores for conjunctivae, chemosis and cornea exceed the thresholds for classifying a test substance as "irritating" as set by the EC Guideline 93/21. Criteria for corrosion are not fulfilled.
Therefore the test substance ethyl cyanoacetate is an irritant according to the EC-guideline 93/21. - Executive summary:
Aim of the study
The aim of this study was to investigate possible irritating or corrosive effects of the undiluted test substance following a single administration into a conjunctival sac of rabbits.
Methods
Methods and investigations were performed in conformance with the OECD Guideline 405, 1987.
Administration of the test substance
The equivalent of 0.1 cm³ ethyl cyanoacetate was instilled into the conjunctival sac of one eye of each of 3 rabbits. The eyes were rinsed with warm isotonic solution of sodium chloride after 24 h.
Investigations
Eye examination: 1, 24,48, 72 h and 6,8, d after the administration
Results
General signs
No other than ocular lesions were noted.
Eye examination
A clearly hyperemia respectively a redness and a light swelling of the conjunctivae was observed in all animals after one hour exposition.
Cornea: 1 animal was normal at any time. In 2 animals diffuse opacity of the cornea was present from 48 h to 72 h p.a.
Iris: 2 animals were normal at any time. In one animal mild lesions were observed after 24 h p.a.
Conjunctivae: Pronounced redness was observed in all animals already 1 h p.a.
Chemosis: A light swelling was seen in all animals already 1 h p.a. In 1 animal a clearly swelling with a partial ecetropion of the lid was observed 24 h p.a.
The third animal was without findings after 48 h p.a., the other two animals were without findings after 8 d p.a.
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