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EC number: 287-820-4 | CAS number: 85586-18-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 to 14 May 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was conducted in accordance with international guidelines and in accordance with GLP. All relevant validity criteria were met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Remarks:
- Statement
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
n/a - Analytical monitoring:
- no
- Details on sampling:
- n/a
- Vehicle:
- no
- Details on test solutions:
- Previous studies had identified the 48 hour EC50 as being approximately 100mg/L, therefore definitive test concentrations were prepared as 0 (control), 25, 50, 100, 200, 400 and 800 mg/L.
The PR-4758 is stated to contain 45% active monoethylene glycol, therefore two further controls were prepared containing 45mg/L and 360 mg/L ethylene glycol. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST SPECIES
Test organism: Daphnia magna
Source: Shell Research Laboratories
Date of gravid isolation: 11 May 2003
Age of daphnia at start of test: <24 hours
Culture conditions:
- Temperature: 20 +/- 1 deg C
- Dissolved oxygen: >60% ASV
- Photoperiod: 16 hours light and 8 hours dark
Feeding regime:
- Each working day a suspension of Chlorella vulgaris, at a rate of 1mg organic carbon per litre of culture water. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 231 mg/L CaCO3
- Test temperature:
- Temperature: 20 +/- 1 deg C
- pH:
- ph 7.0 - 8.0
- Dissolved oxygen:
- >60% ASV
- Salinity:
- n/a
- Nominal and measured concentrations:
- Nominal concentrations at 0 (control), 25, 50, 100, 200, 400 and 800 mg/L.
- Details on test conditions:
- For each test concentration five Daphnia were transfered in a minimum amount of culture water ( < 0.5 mL) into four test vessels (50 mL) with 25 mL of test solution. The four controls were prepared with 25 mL of dilution water. Aerial contamination and evapouration were limited by placing a trasparent perspex sheet over the tops of the test vessels. The pH, dissolved oxygen and temperature were measured prior to initiating the test and at the end of the test. (See details in Water Quality Table).
The number of immobilised Daphnia were recorded at 24 and 48 hours, and EC 50 values calculated using ToxCalc v5. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 703 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI = 500-1418 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 105 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI = 79 - 138 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- There were no mortalities in the dilution water control, or the monoethylene glycol (PR-4758 solvent) control tests.
- Results with reference substance (positive control):
- n/a
- Reported statistics and error estimates:
- See summary table of effect concnetrations
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 hour EC50 value for PR-4758 to Daphnia magna was 105 mg/L, and the 48 hour NOEC was 25 mg/L.
Allwater quality measurements were within acceptabel limits, and the water and solvent (MEG present in PR-4758) controls resulted in no mortalities, therefore the solvent present in PR-4758 does not contribute to the toxicity observed. - Executive summary:
Substance: PR-4758
Chemex reference: Sample: ECO040309; Study: ENV6432
Test species: Daphnia magna
Test type: Acute toxicity: 48 hour EC50
Test Conditions: Static test conditions according to SOP E201 based upon OECD 202 guideline
Test Period: 12 May to 14 May 2003
Test performed at: Chemex Environmental International Limited, Bar Hill Business Park, 37 Saxon Way, Bar Hill, Cambridge, CB3 8EL, UK
Results:
Period of Exposure (hours)
EC50Value (mg/L)
95 % Confidence Limits (mg/L)
24
703
500 - 1418
48
105
79 - 138
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 June 2002 to 05 July 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study was conducted in accordance with international guidelines and in accordance with GLP. All relevant validity criteria were met.
- Qualifier:
- according to guideline
- Guideline:
- other: ISO/CD 14669 and in accordance with PARCOM Ring Test Workshop 1990
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- GLP Statement
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
n/a - Analytical monitoring:
- no
- Details on sampling:
- n/a
- Vehicle:
- no
- Details on test solutions:
- Test substance was dissolved in filtered and aerated seawater. A stock solution was prepared at the highest test concentration of 54mg/L and test solutions of 2, 6 and 18 prepared from dilutions of the stock solution.
- Test organisms (species):
- other: Acartia tonsa
- Details on test organisms:
- TEST SPECIES
Test organism: Acartia tonsa
Source: University of Copenhagen, Laboratory of Marine Biology, Helsingor, Denmark
Date organisms delivered to laboratory: 12 April 2002
Age of copepods at start of test: 16 day
Life stage: Adult - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- mortality only
- Hardness:
- no data
- Test temperature:
- 18.0 - 19.6 deg C
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 2, 6, 18 & 54 mg/L
- Details on test conditions:
- Four replicates at four concentrations and four controls were tested. One test concentration (1.0 mg/L) of the reference substance 3,5-Dichlorophenol was also used.
A range finding test was not performed, but the definitive test concentrations selected based on the results from the Skeletonema costatum study results. The number of mortalities after 24 & 48 hours were recorded. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 48 h
- Dose descriptor:
- LC10
- Effect conc.:
- 29.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 31 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- 32.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Test Validation Criteria:
- The dissolved oxygen concentration at the end of the test is greater then or equal to 4 mg/L
- The percentage lethality of the control is less than or equal to 10%
- The concentration of 1.0 mg/L 3,5-Dichlorophenol results in an effect between 20 and 80% lethality.
- The temperature has been in the range 20.0 deg C +/- 2.4 deg C
In this test the control parameters yielded the following values:
- DO concentration = 7.2 mg/L
- % Lethality of the control = 0%
- Lethality of 1.0 mg/L 3,5-Dichlorophenol = 75%
- Temperature variations during the test = 18.0-19.6 deg C - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 hour LC50 was 31.0 mg/L. 95% confidence interval could not be calculated. The NOEC value was 18 mg/L.
- Executive summary:
A marine invertebrate 48 hour acute toxicity test was performed using the species Acartia tonsa, and the test substance PR-4758.
The control and reference substance validation criteria were met, and based on the four test concentrations employed the LC50 was calculated as 31.0 mg/L.
Referenceopen allclose all
Cumulative percent immobilisation
Concentration (mg/L) |
Number immobilised | % immobilised | ||
24 hours | 48 hours | 24 hours | 48 hours | |
Contol I (water) | 0 | 0 | 0 | 0 |
Control II (MEG) | 0 | 0 | 0 | 0 |
Control III (MEG) | 0 | 0 | 0 | 0 |
25 | 0 | 0 | 0 | 0 |
50 | 0 | 5 | 0 | 25 |
100 | 0 | 12 | 0 | 60 |
200 | 2 | 15 | 10 | 75 |
400 | 7 | 17 | 35 | 85 |
800 | 10 | 20 | 50 | 100 |
Test concentration and Mortality Table
Test Conc (mg/L) | Parallel | No. or organisims per vessel | Mortalities 24 hours | Mortalities 48 hours |
Control | 1 | 5 | 0 | 0 |
2 | 5 | 0 | 0 | |
3 | 5 | 0 | 0 | |
4 | 5 | 0 | 0 | |
2 | 1 | 5 | 0 | 0 |
2 | 5 | 0 | 0 | |
3 | 5 | 0 | 0 | |
4 | 5 | 0 | 0 | |
6 | 1 | 5 | 0 | 0 |
2 | 5 | 0 | 0 | |
3 | 5 | 0 | 0 | |
4 | 5 | 0 | 0 | |
18 | 1 | 5 | 0 | 0 |
2 | 5 | 0 | 0 | |
3 | 5 | 0 | 0 | |
4 | 5 | 0 | 0 | |
54 | 1 | 5 | 5 | 5 |
2 | 5 | 5 | 5 | |
3 | 5 | 5 | 5 | |
4 | 5 | 5 | 5 |
Description of key information
96 hour EC50 = 105 mg/L; OECD 202; WJ Craig (2003)
96 hour EC50 = 31 mg/L; ISO/CD 14669 and in accordance with PARCOM Ring Test Workshop 1990; Haug (2002)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 105 mg/L
Marine water invertebrates
Marine water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 31 mg/L
Additional information
A key study for both freshwater and marine compartments is available. Both studies were conducted in accordance with international guidelines and GLP.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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