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EC number: 269-212-0 | CAS number: 68201-32-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-07-07 to 2004-07-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP study performed according to Guideline United Kingdom Proposal to ISO TC147/SC5/WG2 "Water Quality, Determination of Acute Lethal Toxicity to Marine Copepods (Copepoda, Crustacea)".
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: The method used followed the recommendations of the United Kingdom Proposal to ISO TC147/SC5/WG2 "Water Quality, Determination of Acute Lethal Toxicity to Marine Copepods (Copepoda, Crustacea)"
- Deviations:
- yes
- Remarks:
- During holding, the test organisms were fed a mixture of different marine algae other than those specified in the test method. This deviation is judged not to affect the results of the study.
- Principles of method if other than guideline:
- N/A
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Department of Health of the Government of the United Kingdom
Test material
- Reference substance name:
- Asphalt, sulfonated, sodium salt
- EC Number:
- 269-212-0
- EC Name:
- Asphalt, sulfonated, sodium salt
- Cas Number:
- 68201-32-1
- Molecular formula:
- UVCB
- IUPAC Name:
- Asphalt, sulfonated, sodium salt
- Details on test material:
- - Name of test material (as cited in study report): Na+ sulfonated asphalt
- Molecular formula (if other than submission substance): N/A
- Molecular weight (if other than submission substance): N/A
- Smiles notation (if other than submission substance): N/A
- InChl (if other than submission substance): N/A
- Structural formula attached as image file (if other than submission substance): N/A
- Substance type: active
- Physical state: black powder
- Analytical purity: N/A
- Impurities (identity and concentrations): N/A
- Composition of test material, percentage of components: N/A
- Isomers composition: N/A
- Purity test date: N/A
- Lot/batch No.: N/A
- Stability under test conditions: N/A
- Storage condition of test material: N/A
- Other: Test material supplied by Sponsor.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - The test material concentrations in test preparations were not determined by analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - The test water was prepared using a commercially available formulation (Tropic Marin® Synthetic Sea Salt).
- In the definitive test, 731 g was dispersed in a final volume of 20 L of water giving a nominal salinity of 31 parts per thousand, and the pH was adjusted to 8.0 using 10 M HCl.
- Test solutions were prepared by directly adding 1000 mg of test material to the synthetic seawater, which was dispersed by ultrasonication for 30 minutes and the volume adjusted to 1 liter to give the 1000 mg/L test concentration. Aliquots of the 1000 mg/L test concentration were used to prepare the remaining test concentrations: 10, 18, 32, 56, 100, 180, 320 and 560 mg/L. Each prepared concentration was inverted several times to ensure adequate mixing and homogeneity.
Test organisms
- Test organisms (species):
- other: Acartia tonsa
- Details on test organisms:
- TEST ORGANISM
- Common name: Marine copepod
- Strain: N/A
- Source: Guernsey Sea Farms, Vale, Guernsey, UK
- Age at study initiation (mean and range, SD): 11 - 14 days were supplied for use in the definitive test and positive control.
- Weight at study initiation (mean and range, SD): N/A
- Length at study initiation (length definition, mean, range and SD): N/A
- Valve height at study initiation, for shell deposition study (mean and range, SD): N/A
- Peripheral shell growth removed prior to test initiation: N/A
- Method of breeding: N/A
- Feeding during test
- Food type: N/A
- Amount: N/A
- Frequency: Not fed during test
ACCLIMATION
- Acclimation period: 1 day
- Acclimation conditions (same as test or not): sameas test; The test animals were held in 20 +/- 1 deg. C.
- Type and amount of food: Held in sea water with a salinity of 33 +/- 2 parts per thousand and fed on a mixed culture of marine algae. Algal mix used as food consisted of Pavlova lutheri and Tetraselmis sueccica supplied by Guernsey Sea Farms, rather than Skeletonema sp. and Rhodomonas sp. as stated in the standard test method. This deviation was considered to have no effect on the test results.
- Feeding frequency: N/A
- Health during acclimation (any mortality observed): N/A
QUARANTINE (wild caught)
- Duration: N/A
- Health/mortality: N/A
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- N/A
Test conditions
- Hardness:
- N/A
- Test temperature:
- - The water temperature was recorded daily throughout the test in each replicate test vessel.
- The test was carried out in a temperature-controlled room at 21.7 - 22.3 deg. C. - pH:
- - The pH was recorded at test start and termination in each replicate test vessel.
- Dissolved oxygen:
- - The test vessels were not aerated during the test.
- The DO concentrations were recorded at test start and termination in each replicate test vessel. - Salinity:
- - 31 ppt in dilution water
- Nominal and measured concentrations:
- - The test concentrations used in the definitive test were based on the results of a range-finding test.
- Based on the results of the range-finding test, the following nominal concentrations were chosen for the definitive test: 10, 18, 32, 56, 100, 180, 320, 560, and 1000 mg/L.
- The positive control was tested at: 0.56, 1.0, 1.8, 3.2, 5.6, 10, 18, 32, and 56 mg/L. - Details on test conditions:
TEST SYSTEM
- Test vessel: 50 mL jars
- Type (delete if not applicable): closed- covered to reduce evaporation
- Material, size, headspace, fill volume: 50 mLglass jars, 25 mL test solution
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): N/A
- Biomass loading rate: N/A
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic seawater made with Tropic Marin seasalt
- Total organic carbon: N/A
- Particulate matter: N/A
- Metals: N/A
- Pesticides: N/A
- Chlorine: N/A
- Alkalinity: N/A
- Ca/mg ratio: N/A
- Conductivity: N/A
- Culture medium different from test medium: N/A
- Intervals of water quality measurement: N/A
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light, 8 h dark, 20 min dawn and dusk transitions
- Light intensity: N/A
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, at 24 and 48 h. At 320, 560 and 1000 mg/L, the organisms were removed from the test vessels and placed in a Petri dish during the 24 and 48 h observations due to the turbidity of the test preparations.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline: N/A
- Range finding study
- Test concentrations: 0.1, 1.0, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: yes- Reference substance (positive control):
- yes
- Remarks:
- : potassium dichromate - Exposure conditions were the same as those in the definitive test with the exception that the temperatures were shown to range from 21.7 - 22.2 deg C.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: No CL
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 380 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL= 330 - 440 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Details on results:
- MORTALITY:
- At 24 h, less than 50% mortality was observed at the highest test concentration of 1000 mg/L, therefore, it was not possible to calculate an LC50 value from the data by the probit method.
- At 48 h, there was no mortality at 10 - 180 mg/L, with increasing mortality at higher concentrations.
MATERIAL SOLUBILITY:
- The turbidity of the dispersion and amount of precipitate increased with increasing concentration. The 10 mg/L treatment was a clear colorless solution throughout the duration of the test. The other treatments were observed to be brown dispersions with fine brown particles in suspension at 0 hours; these particles settled to the bottom of the vessel during the test.
OBSERVATIONS ON THE TEST ORGANISMS:
- It was confirmed that no TS adhered to the test organisms in the definitive test. Thus, mortality was not attributable to a physical effect.
PHYSICO-CHEMICAL MEASUREMENTS:
- pH ranged from 8.1 - 8.2 in all treatments throughout the test.
- Dissolved oxygen ranged from 52% to 84% of the air saturation value (ASV) at 0 hours, with decreasing ASV at higher test concentrations. At 48 hours, DO was 70 - 78% ASV at all test concentrations.
- Temperature ranged from 21.7 - 22.2 in all treatments throughout the test. - Results with reference substance (positive control):
- - The 24 h LC50 was: 35 mg/L (95% CI 29 - 47 mg/L).
- The 48 h LC50 was: 4.5 mg/L (95% CI 3.8 - 5.5 mg/L).
POSITIVE CONTROL:
- The results from the positive control were within the normal range for this potassium dichromate.
- The 24 h NOEC was: 10 mg/L.
- The 48 h NOEC was: 0.1 mg/L.
PHYSICO-CHEMICAL MEASUREMENTS:
- The temperature was maintained at 21.7 - 22.3 deg C throughout the test (this is above the temperature range in the protocol; however, it was determined that there were no temperature-related effects on the test organisms).
- No treatment-related differences in pH.
- A concentration-dependent difference in oxygen concentration was observed throughout the test. - Reported statistics and error estimates:
- The LC50 values and associated confidence limits at 24 and 48 hours were calculated by the maximum-likelihood probit method (Finney 1971) using the ToxCalc computer software package (ToxCalc 1991). Probit analysis is used were two or more partial responses to exposure are shown.
Any other information on results incl. tables
Table 1. Cumulative Mortality in the Definitive Test.
Cumulative Mortality Data in the Definitive Test |
||||||||||||
Nominal Concentration (mg/L) |
Cumulative Mortalities Initial Population: 5 Per Replicate |
|||||||||||
24 Hours |
48 Hours |
|||||||||||
R1 |
R2 |
R3 |
R4 |
Total |
% |
R1 |
R2 |
R3 |
R4 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
18 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
32 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
56 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
180 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
320 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
2 |
2 |
6 |
30 |
560 |
0 |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
4 |
4 |
18 |
90 |
1000 |
3 |
2 |
2 |
1 |
8 |
40 |
5 |
5 |
5 |
6 |
20 |
100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- sufficient survival in controls
- Conclusions:
- The 48 hr LC 50 of the test material to the marine copepod Acartia tonsa has been found to be 380 mg/L with 95% CL of 330 - 440 mg/L. The NOEC at 48 h was 180 mg/L.
- Executive summary:
N/A
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