3,4-dichlorobenzonitrile

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

June 1981

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

There is no data available on skin irritation/corrosion testing with 3,4-Dichlorobenzonitrile. In order to classify the substance according to GHS relevant endpoint data available for 2,6-Dichlorobenzonitrile was read-acrossed to 3,4-Dichlorobenzonitrile based on structural similarities. 2,6-Dichlorobenzonitrile is as well as 3,4-Dichlorobenzonitrile, is a disubstituted Benzonitrile. Both substances only differ in the position of the chloro substituents. Furthermore both substances are solid, practically insoluble in water and have a water octanol partition coefficient of 2.2 and 2.8. This might perhaps also lead to a similiar skin irritation/corrosion potential of both substances.
for Justification of read across see section 13.2

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Dichlobenil

EC Number:

214-787-5

EC Name:

Dichlobenil

Cas Number:

1194-65-6

Molecular formula:

C7H3Cl2N

IUPAC Name:

2,6-dichlorobenzonitrile

Details on test material:

- Name of test material (as cited in study report): 2,6-Dichlorbenzonitril

Test animals

Species:

rabbit

Strain:

other: Russian White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: own breed
- Weight at study initiation: at least 2.5 and 2.8 kg
- Housing: during the post oberservation period the animals were housed in V2A-steelcages with a floor area of 0.4 m2.
- Diet (e.g. ad libitum): KF-G 774 of Laboratorium für Pharmakologie und Toxikologie, 2104 Hamburg 92, Germany) ad libitum
- Water (e.g. ad libitum): tap water ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 +/- 3 °C
- Humidity (%): 60 +/- 5 %
- Photoperiod (hrs dark / hrs light): 12 hours light (150 Lux), 12 hours dark

Test system

Type of coverage:

not specified

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 g

Duration of treatment / exposure:

4 hours

Observation period:

30-60 minutes and 24, 48, 72 and 96 hours after exposure

Number of animals:

6 rabbits (3 male and 3 female)

Details on study design:

TEST SITE
- Area of exposure: max. 6 cm2 of the shaved dorsum skin
- Type of wrap if used: linen lobe with plastic film and bandage (Leukoplast, P. Beiersdorf & Co. AG, Unnastraße 48, 2000 Hamburg, Germany)

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data
- Time after start of exposure: 4 hours

SCORING SYSTEM:
Skin reaction erythema and eschar generation:
0: no erythema
1: very slight, barely perceptible erythema
2: distinctive erythema
3: moderate till serious erythema
4: serious (deep red) erythema till light eschar generation (serious damage)

skin reaction edema generation:
0: no edema
1: very slight, barely perceptible erythema
2: moderate edema with clear definition
3: moderate edema (swelling approx. 1 mm)
4: serious edema (swelling greater than 1 mm and overlapping the area of exposure)

Furthermore the appearance of necrosis was examined.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Remarks on result:

no indication of irritation

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Remarks on result:

no indication of irritation

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

2,6-Dichlorobenzonitrile was tested to be not skin irritating in a test equivalent to OECD 404.

Executive summary:

As there is no data available on skin irritation testing with 3,4 -Dichlorobenzonitrile relevant endpoint data available for 2,6 -Dichlorobenzonitrile was read-acrossed to 3,4 -Dichlorobenzonitrile in order to classify the substance (without any unecessary animal testing) according to GHS. The Read-Across approach was based on structrual and physico chemical similarities (see rational for reliablitity).

2,6 -Dichlorobenzontrile was tested to be not skin irritating in a test equivalent to OECD 404. This agrees with the non skin irritating property of 2 -Chlorobenzonitrile. Therefore 3,4 -Dichlorobenzonitrile was also classified as non irritating to skin.