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EC number: 218-664-7 | CAS number: 2212-81-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989.10.19 to 1989.11.02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was conducted in accordance with a recognized scientific procedure for biodegradation and is based on OECD 209 EEC Part C, Methods for the determination of ecotoxicity, biodegradation, activated sludge respiration inhibition test.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- See "Any other information on materials and methods incl. tables"
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No surrogate or test material was used. - Analytical monitoring:
- not specified
- Details on sampling:
- No data.
- Vehicle:
- yes
- Details on test solutions:
- Stock solution
The test substance was adsorbed onto silica gel using a stock solution of 10 g/litre in dichloromethane. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Secondary activated sludge was collected from the RZWI Nieuwgraaf in Duiven (1989.10.27). This activated sludge plant predominantly treats domestic wastewater. Prior to use, the activated sludge was diluted with tap water (1.5x) and homogenized for two minutes in a lab blender 400. The activated sludge concentration in the incubation vessels was 0,5 g dry weight/litre.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Post exposure observation period:
- No post exposure observation period was realised.
- Hardness:
- No data
- Test temperature:
- 20°C
- pH:
- The pH of the reaction mixture before the respiration measurement varies from 7.5 to 7.9.
- Dissolved oxygen:
- The respiration rates of the activated sludge were measured in a Biological. Oxygen Monitor YSI 5300 (Yellow Springs Instruments, Ohio, USA). The BOM consisted of a thermostated vessel with a magnetic stirrer and an oxygen electrode. The vessel is tightly closed by the electrode to measure the oxygen depletion. The volume of the vessel was 10 ml and the temperature in the vessel was 20°C.
- Salinity:
- The test was performed in freshwater.
- Nominal and measured concentrations:
- No data. Results are expressed as nominal concentrations.
- Details on test conditions:
- Test procedure
The activated sludge respiration inhibition test was performed according to EEC (4.1) and OECD test guidelines (4.2). In this test, the respiration rate of activated sludge fed with a standard amount of synthetic sewage is measured after a contact time of 30 minutes. The respiration rate of the same activated sludge in the presence of various concentrations of the test substance under otherwise identical conditions is also measured. The inhibitory effect of the test substance at a particular concentration is expressed as a percentage of the mean respiration rates of the controls. One deviation introduced and some aspects of the test are presented in "Any other information on materials and methods incl. tables".
Test conditions
The homogenized activated sludge was incubated in a shaking water bath (100 Hz, 20°C) for 30 minutes with various concentrations of the test compound and synthetic sewage.
- Reference substance (positive control):
- yes
- Remarks:
- instead of 3,5-dichiorophenol, 2,4,5-trichlorophenol was used as a reference compound because 3,5-dichiorophenol was not commercially available. 2,4,5-Trichlorophenol was chosen for its structural resemblance and is expected to give the same result
- Duration:
- 30 min
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Duration:
- 30 min
- Dose descriptor:
- EC0
- Effect conc.:
- > 1 000 mg/L
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Details on results:
- The results are presented in the Table in "Remarks on results including tables and figures". No inhibition of the respiration rate occurred at 1000 mg/litre of bis (tert-butyl peroxy isopropyl)benzene adsorbed onto silica gel. This result shows that [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide, at the obtained distribution, is not toxic for activated sludge.
- Results with reference substance (positive control):
- See table in "Remarks on results including tables and figures"
- Reported statistics and error estimates:
- No data.
- Validity criteria fulfilled:
- yes
- Remarks:
- See Conclusions
- Conclusions:
- The validity of the test is shown by two criteria. Firstly, the control respiration rates are within 0% of each other and secondly, the EC50 of the reference compound is within the described range (5-30 mg/litre). Furthermore, the silica gel used is not toxic for the activated sludge. The pH of the reaction mixture before the respiration measurement varies from 7.5 to 7.9. The test substance was not soluble in water and therefore the activated sludge was exposed to [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide adsorbed onto silica gel at a concentration of 1 g/litre. By this exposure no effect was recorded indicating that the obtained distribution of the [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide in the water system did not inhibit the activated sludge, EC 50 > 1000 mg/l.
- Executive summary:
- In order to predict effects of chemicals in the environment and in biological wastewater treatment plants, an activated sludge respiration inhibition test was performed according to OECD 209 guideline and GLP requirements. The test substance was not soluble in water and therefore the activated sludge was exposed to [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide adsorbed onto silica gel at a concentration of 1 g/litre. By this exposure no effect was recorded indicating that the obtained distribution of [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide in the water system did not inhibit the activated sludge. Validity criteria were fulfilled.
Reference
Respiration rates of the activated sludge(R5) and inhibition percentaqes calculated at 1 q/litre of the test compound
concentration |
pH |
respiration rate |
inhibition |
(mg/litre) |
start |
(mg02/litre min) |
(%) |
control (Rci) |
7.9 |
0.37 |
-‑ |
control (Rc2) |
7.9 |
0.36 |
-‑ |
1000 |
7.5 |
0.36 |
1 |
Respiration rates of the activated sludqe and inhibition percentages calculated at various concentrations of 2,4,5-trichlorophenol (reference substance)
concentration |
pH |
respiration rate |
inhibition |
(mg/litre) |
start |
(mg02/litre min) |
(%) |
control |
7.9 |
0.37 |
-‑ |
control |
7.9 |
0.37 |
-‑ |
5 |
7.8 |
0.30 |
16 |
10 |
7.9 |
0.20 |
46 |
20 |
7.9 |
0.13 |
66 |
40 |
7.9 |
0.05 |
86 |
Respiration rates of the activated sludqe and the inhibition percentage calculated for silica gel
concentration |
pH |
respiration rate |
inhibition |
(mg/litre) |
start |
(mg 02/litre min) |
(%) |
control (Rc1) |
7.9 |
0.37 |
-‑ |
control (Rc2) |
7.9 |
0.36 |
-‑ |
silica gel |
7.5 |
0.38 |
0 |
Description of key information
In order to predict effects of [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide in the environment and in biological wastewater treatment plants, an activated sludge respiration inhibition test was performed according to OECD 209 guideline and GLP requirements. The test substance was not soluble in water and therefore the activated sludge was exposed to [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide adsorbed onto silica gel at a concentration of 1 g/litre. By this exposure no effect was recorded indicating that the obtained distribution of [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide in the water system did not inhibit the activated sludge and EC50 > 1000 mg/l.
Key value for chemical safety assessment
Additional information
In order to predict effects of chemicals in the environment and in biological wastewater treatment plants, an activated sludge respiration inhibition test was performed according to OECD 209 guideline and GLP requirements. The test substance was not soluble in water and therefore the activated sludge was exposed to [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide adsorbed onto silica gel at a concentration of 1 g/litre. By this exposure no effect was recorded indicating that the obtained distribution of [1,3-phenylenebis(1-methylethylidene)]bis[tert-butyl] peroxide in the water system did not inhibit the activated sludge. Validity criteria were fulfilled.
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