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EC number: 263-272-1 | CAS number: 61847-48-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Sediment toxicity
Administrative data
Link to relevant study record(s)
- Endpoint:
- sediment toxicity: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-05-26 to 2008-06-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study compliant to GLP
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 225: Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment (October 2007)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- GLP according to German Chemikaliengesetz and Directive 88/320/EEC
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on sediment and application:
- PREPARATION OF SPIKED SEDIMENT
- Details of spiking: The test substance concentration was weighed out, dissolved in 15 mL acetone and mixed thoroughly with 60 g quartz sand (10 g quartz sand per replicate) in a glass vessel. After complete evaporation of the solvent, the dry quartz sand was given to the sediment. Residues of the dry quartz sand sticking at the glas vessel were rinsed off with 50 mL demineralised water per treatment. Thereafter, the sediment was thoroughly mixed with a long handled blender for at least three minutes and filled into the test vessels.
- Equilibration time: 2 days prior to insertion of test organisms
- Equilibration conditions: Same as test
- Controls: Controls with test sediment and quartz sand. Vehicle controls with test sediment and quartz sand treated with acetone.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): organic solvent, acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 15 mL per 60 g quartz sand (6 replicates)
- Evaporation of vehicle before use: Yes, complete evaporation
- Test organisms (species):
- Lumbriculus variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: Lumbriculus variegatus (Müller), Lumbriculidae, Oligochaeta
- Source: Bundesanstalt für Gewässerkunde (BfG), Am Mainzer Tor 1, 56068 Koblenz, Germany
- Age of test organisms (mean and range, SD): Synchronized from cultured organisms 13 days before test insertion
- Breeding conditions: 20 +/- 2 deg C, diffuse light (100 - 1000 lx, 16 h photoperiod) dissolved oxygen concentration > 60 % of the air saturation value
- Handling of worms: 13 days before insertion into the test, the worms were synchronized. Therefore, a sufficient amount of worms was taken from the breeding and cut into half.. After regeneration of heads, the worms were inserted into the test.
- Age of animals at beginning of exposure: 13 days after synchronisation, completely regenerated head region. Synchronization was done to minimize uncontrolled regeneration and reproduction during the test.
- Feeding during test
- Food type: Powder of Urtica dioica (Heinrich Klein GmbH & Co KG, 97525 Schwebheim, Germany)
- Amount: 0.5 % Urtica powder
- Frequency: Urtica powder was mixed into the sediment once at test start.
ACCLIMATION
- Acclimation period: 13 days (after synchronization)
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Same as test
- Feeding frequency: Same as test (Once)
- Health during acclimation (any mortality observed): No mortality observed - Study type:
- laboratory study
- Test type:
- static
- Water media type:
- freshwater
- Type of sediment:
- artificial sediment
- Limit test:
- yes
- Duration:
- 28 d
- Exposure phase:
- total exposure duration
- Hardness:
- Day 0: Control: > 356 mg/L CaCO3, Solvent Control: 340 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3
Day 28: Control: > 356 mg/L CaCO3, Solvent Control: > 356 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3 - Test temperature:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 Temperature [°C] 21.1 21.0 20.9
7 Temperature [°C] 21.9 21.2 21.9
14 Temperature [°C] 22.0 20.2 20.2
21 Temperature [°C] 21.8 21.8 21.4
28 Temperature [°C] 21.8 21.2 20.8 - pH:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 pH 7.74 7.82 7.88
7
pH 7.86 7.86 7.88
14
pH 7.76 7.79 7.86
21
pH 7.58 7.87 8.01
28
pH 8.22 8.27 8.29 - Dissolved oxygen:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 O2 [mg/L] 6.16 6.78 6.37
7 O2 [mg/L] 6.06 6.05 6.40
14 O2 [mg/L] 6.90 6.22 6.71
21 O2 [mg/L] 7.37 7.11 7.05
28
O2 [mg/L] 7.30 8.04 7.96 - Salinity:
- Not measured, freshwater conditions
- Ammonia:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 NH4-N [mg/L] 0.803 0.622 0.797
2 NH4-N [mg/L] 0.114 < 0.02* < 0.02*
5 NH4-N [mg/L] 0.039 0.031 0.113
8 NH4-N [mg/L] 0.045 0.039 0.044
9 NH4-N [mg/L] 0.091 0.050 0.031
12 NH4-N [mg/L] 0.064 0.060 0.068
15 NH4-N [mg/L] 0.089 0.241 0.063
16 NH4-N [mg/L] 1.03 0.715 0.121
19 NH4-N [mg/L] > 2.5* > 2.5* > 2.5*
NH4-N [mg/L] 5.71 5.35 4.29
23 NH4-N [mg/L] 4.84 4.75 5.42
26 NH4-N [mg/L] 6.91 6.01 5.85
28 NH4-N [mg/L] 6.76 6.58 4.30
- Nominal and measured concentrations:
- ominal concentration limit concentration: 1000 mg test substance/kg sediment DW.
The submission substance has a low water solubility and due to its sorption to particular matter, low volatility and very low biotic and abiotic degradation. It resides predominantly in the solid phase of the sediment. The fraction of the pigment in the overlying water as well as the porewater can be neglected. As no loss of pigment was expected to occur during the exposure period an analytical determination of the pigment in the overlying water and the sediment has been omitted. Based on the fact that the test system consists of 150 mL overlying water 0.008 % pigment would be in the overlying water at saturation and thus 99.992 % remains in the sediment (solids & porewater). - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 250 mL glass beakers, 6 cm diameter
- Sediment volume: 45 g DW per vessel, ca. 2 cm from the ground
- Overlying water volume: 150 mL
- Depth of sediment and overlying water: Sediment depth ca. 2 cm, water depth ca. 10 cm
- Aeration: yes
- Aeration frequency and intensity: Gentle aeration (2 bubbles per second)
- Replacement of evaporated test water, if any: Yes, once weekly
EXPOSURE REGIME
- No. of organisms per container (treatment): 10 per container (60 per treatment)
- No. of replicates per treatment group: 6 replicates per treatment group
- No. of replicates per control / vehicle control: 6 replicates per control/vehicle control
- Feeding regime: Once at test start
- Type and preparation of food: Powder of Urtica dioica mixed into the sediment before insertion of test organisms
- Amount of food: 0.5 %
OVERLYING WATER CHARACTERISTCS
- Type of water (e.g. deionized, ground water, sea water, Elendt medium acc. to OECD 219): Reconstituted water acc. to OECD 203
CHARACTERIZATION OF (ARTIFICIAL; delete if not applicable) SEDIMENT
- Composition (if artificial substrate): 5 % peat air dried and finely ground, 20 % kaolin, 75 % quartz sand, 0.33 % calcium carbonate
Table 1: Sediment Properties at Test Start
Total Organic Carbon [%] pH-value Moisture [%]
Sediment at test start 2.15 6.74 8.29
OTHER TEST CONDITIONS
Room temperature [°C] Photoperiod [h] Light intensity [lx]
Nominal 20 +/- 2 16 100 - 1000
Actual 18 - 21 16 472 +/- 47.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and behaviour changes (Day 0-28), reproduction and biomass at test end (28 d)
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
1000 mg test substance/kg sediment DW
- Range finding study
- Test concentrations: 10 - 100 - 1000 mg/kg sediment DW
- Results used to determine the conditions for the definitive study:
Concentration Mortality Mean number of worms Mean dry weight
[mg test item/kg sediment DW] [%] [mg]
Control 0 13.5 14.7
Solv. Control 0 14.5 16.0
10 0 13.0 35.4
100 0 11.5 16.8
1000 0 12.0 15.9 - Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality, behaviour, biomass reproduction
- Details on results:
- - Mortality of test animals at end of exposure period: No mortality
- Total mass of test animals at beginning of test: 10 worms were equal to ca. 45.8 mg wet mass
- Morphological abnormalities: None
- Behavioural abnormalities: None - Reported statistics and error estimates:
- One Way Analysis of Variance (ANOVA, Dunnett´s Method) was carried out with worm numbers and biomass data for the determination of statistically significant differences compared to the pooled control. Prior to the ANOVA a normality test and an equal variance test were conducted. P-values for both normality and equal variance test were 0.05. The alpha-value (acceptable probability of incorrectly concluding that there is a difference) for ANOVA was alpha = 0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this sedmient-water Lubriculus toxicity test according to OECD 225 the submission substance caused no significant effects on mortality, behaviour, biomass and reproduction at the limit test item concentration of 1000 mg/kg sediment DW. Thus, the NOEC was determined to be equal or larger than 1000 mg/kg sediment DW.
- Executive summary:
The effects of the test item on the oligochaete Lumbriculus variegatus in a water-sediment system were determined. The study was carried out according to OECD Guideline 225 (2007) compliant to GLP (reliability category 1). Test duration was 28 days after insertion of the test organisms. A limit test with the nominal test concentration of 1000 mg/kg sediment dry weight (DW) was performed by spiking the test item into the sediment. Six replicates per control, vehicle control and limit test item concentration with ten worms per replicate were set up. Water quality parameters as temperature, pH-value, O2-concentration, ammonia and total hardness were determined.
Due to the chemical properties of the test item the fraction of the pigment in the overlying water
as well as in the porewater can be neglected. Thus, no analytical verification of the test item
was performed.
After 28 days no mortality or significant behavioural changes of L. variegatus were observed at the limit test item concentration of 1000 mg/kg sediment DW. Also, the biomass was not significantly reduced compared to the pooled control after 28 days of exposure to the test item. L. variegatus reproduced without any significant differences between the pooled control and the limit test item concentration of 1000 mg/kg sediment DW (Table 1).
Table 1: Summary of all Significant Effects
Effects
Test item concentration
Significance of effects
[mg/kg sediment DW]
Mortality
> 1000
Behaviour
> 1000
Biomass
> 1000
Reproduction
> 1000
NOEC
(mortality, behaviour, biomass, reproduction)
>= 1000
- Endpoint:
- sediment toxicity: long-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2008-05-26 to 2008-06-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study compliant to GLP
- Justification for type of information:
- See Rationale and Justification for the Analogue Read-Across Approach for the registration of the Nanoform of Pigment Red 188 using Pigment Red 22, Pigment Red 112 and Pigment Orange 38 as Source Substances (Chapter 13)
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 225: Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment (October 2007)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- GLP according to German Chemikaliengesetz and Directive 88/320/EEC
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on sediment and application:
- PREPARATION OF SPIKED SEDIMENT
- Details of spiking: The test substance concentration was weighed out, dissolved in 15 mL acetone and mixed thoroughly with 60 g quartz sand (10 g quartz sand per replicate) in a glass vessel. After complete evaporation of the solvent, the dry quartz sand was given to the sediment. Residues of the dry quartz sand sticking at the glas vessel were rinsed off with 50 mL demineralised water per treatment. Thereafter, the sediment was thoroughly mixed with a long handled blender for at least three minutes and filled into the test vessels.
- Equilibration time: 2 days prior to insertion of test organisms
- Equilibration conditions: Same as test
- Controls: Controls with test sediment and quartz sand. Vehicle controls with test sediment and quartz sand treated with acetone.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): organic solvent, acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 15 mL per 60 g quartz sand (6 replicates)
- Evaporation of vehicle before use: Yes, complete evaporation
- Test organisms (species):
- Lumbriculus variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: Lumbriculus variegatus (Müller), Lumbriculidae, Oligochaeta
- Source: Bundesanstalt für Gewässerkunde (BfG), Am Mainzer Tor 1, 56068 Koblenz, Germany
- Age of test organisms (mean and range, SD): Synchronized from cultured organisms 13 days before test insertion
- Breeding conditions: 20 +/- 2 deg C, diffuse light (100 - 1000 lx, 16 h photoperiod) dissolved oxygen concentration > 60 % of the air saturation value
- Handling of worms: 13 days before insertion into the test, the worms were synchronized. Therefore, a sufficient amount of worms was taken from the breeding and cut into half.. After regeneration of heads, the worms were inserted into the test.
- Age of animals at beginning of exposure: 13 days after synchronisation, completely regenerated head region. Synchronization was done to minimize uncontrolled regeneration and reproduction during the test.
- Feeding during test
- Food type: Powder of Urtica dioica (Heinrich Klein GmbH & Co KG, 97525 Schwebheim, Germany)
- Amount: 0.5 % Urtica powder
- Frequency: Urtica powder was mixed into the sediment once at test start.
ACCLIMATION
- Acclimation period: 13 days (after synchronization)
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Same as test
- Feeding frequency: Same as test (Once)
- Health during acclimation (any mortality observed): No mortality observed - Study type:
- laboratory study
- Test type:
- static
- Water media type:
- freshwater
- Type of sediment:
- artificial sediment
- Limit test:
- yes
- Duration:
- 28 d
- Exposure phase:
- total exposure duration
- Hardness:
- Day 0: Control: > 356 mg/L CaCO3, Solvent Control: 340 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3
Day 28: Control: > 356 mg/L CaCO3, Solvent Control: > 356 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3 - Test temperature:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 Temperature [°C] 21.1 21.0 20.9
7 Temperature [°C] 21.9 21.2 21.9
14 Temperature [°C] 22.0 20.2 20.2
21 Temperature [°C] 21.8 21.8 21.4
28 Temperature [°C] 21.8 21.2 20.8 - pH:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 pH 7.74 7.82 7.88
7
pH 7.86 7.86 7.88
14
pH 7.76 7.79 7.86
21
pH 7.58 7.87 8.01
28
pH 8.22 8.27 8.29 - Dissolved oxygen:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 O2 [mg/L] 6.16 6.78 6.37
7 O2 [mg/L] 6.06 6.05 6.40
14 O2 [mg/L] 6.90 6.22 6.71
21 O2 [mg/L] 7.37 7.11 7.05
28
O2 [mg/L] 7.30 8.04 7.96 - Salinity:
- Not measured, freshwater conditions
- Ammonia:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 NH4-N [mg/L] 0.803 0.622 0.797
2 NH4-N [mg/L] 0.114 < 0.02* < 0.02*
5 NH4-N [mg/L] 0.039 0.031 0.113
8 NH4-N [mg/L] 0.045 0.039 0.044
9 NH4-N [mg/L] 0.091 0.050 0.031
12 NH4-N [mg/L] 0.064 0.060 0.068
15 NH4-N [mg/L] 0.089 0.241 0.063
16 NH4-N [mg/L] 1.03 0.715 0.121
19 NH4-N [mg/L] > 2.5* > 2.5* > 2.5*
NH4-N [mg/L] 5.71 5.35 4.29
23 NH4-N [mg/L] 4.84 4.75 5.42
26 NH4-N [mg/L] 6.91 6.01 5.85
28 NH4-N [mg/L] 6.76 6.58 4.30
- Nominal and measured concentrations:
- ominal concentration limit concentration: 1000 mg test substance/kg sediment DW.
The submission substance has a low water solubility and due to its sorption to particular matter, low volatility and very low biotic and abiotic degradation. It resides predominantly in the solid phase of the sediment. The fraction of the pigment in the overlying water as well as the porewater can be neglected. As no loss of pigment was expected to occur during the exposure period an analytical determination of the pigment in the overlying water and the sediment has been omitted. Based on the fact that the test system consists of 150 mL overlying water 0.008 % pigment would be in the overlying water at saturation and thus 99.992 % remains in the sediment (solids & porewater). - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 250 mL glass beakers, 6 cm diameter
- Sediment volume: 45 g DW per vessel, ca. 2 cm from the ground
- Overlying water volume: 150 mL
- Depth of sediment and overlying water: Sediment depth ca. 2 cm, water depth ca. 10 cm
- Aeration: yes
- Aeration frequency and intensity: Gentle aeration (2 bubbles per second)
- Replacement of evaporated test water, if any: Yes, once weekly
EXPOSURE REGIME
- No. of organisms per container (treatment): 10 per container (60 per treatment)
- No. of replicates per treatment group: 6 replicates per treatment group
- No. of replicates per control / vehicle control: 6 replicates per control/vehicle control
- Feeding regime: Once at test start
- Type and preparation of food: Powder of Urtica dioica mixed into the sediment before insertion of test organisms
- Amount of food: 0.5 %
OVERLYING WATER CHARACTERISTCS
- Type of water (e.g. deionized, ground water, sea water, Elendt medium acc. to OECD 219): Reconstituted water acc. to OECD 203
CHARACTERIZATION OF (ARTIFICIAL; delete if not applicable) SEDIMENT
- Composition (if artificial substrate): 5 % peat air dried and finely ground, 20 % kaolin, 75 % quartz sand, 0.33 % calcium carbonate
Table 1: Sediment Properties at Test Start
Total Organic Carbon [%] pH-value Moisture [%]
Sediment at test start 2.15 6.74 8.29
OTHER TEST CONDITIONS
Room temperature [°C] Photoperiod [h] Light intensity [lx]
Nominal 20 +/- 2 16 100 - 1000
Actual 18 - 21 16 472 +/- 47.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and behaviour changes (Day 0-28), reproduction and biomass at test end (28 d)
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
1000 mg test substance/kg sediment DW
- Range finding study
- Test concentrations: 10 - 100 - 1000 mg/kg sediment DW
- Results used to determine the conditions for the definitive study:
Concentration Mortality Mean number of worms Mean dry weight
[mg test item/kg sediment DW] [%] [mg]
Control 0 13.5 14.7
Solv. Control 0 14.5 16.0
10 0 13.0 35.4
100 0 11.5 16.8
1000 0 12.0 15.9 - Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality, behaviour, biomass reproduction
- Details on results:
- - Mortality of test animals at end of exposure period: No mortality
- Total mass of test animals at beginning of test: 10 worms were equal to ca. 45.8 mg wet mass
- Morphological abnormalities: None
- Behavioural abnormalities: None - Reported statistics and error estimates:
- One Way Analysis of Variance (ANOVA, Dunnett´s Method) was carried out with worm numbers and biomass data for the determination of statistically significant differences compared to the pooled control. Prior to the ANOVA a normality test and an equal variance test were conducted. P-values for both normality and equal variance test were 0.05. The alpha-value (acceptable probability of incorrectly concluding that there is a difference) for ANOVA was alpha = 0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this sedmient-water Lubriculus toxicity test according to OECD 225 the submission substance caused no significant effects on mortality, behaviour, biomass and reproduction at the limit test item concentration of 1000 mg/kg sediment DW. Thus, the NOEC was determined to be equal or larger than 1000 mg/kg sediment DW.
- Executive summary:
The effects of the test item on the oligochaete Lumbriculus variegatus in a water-sediment system were determined. The study was carried out according to OECD Guideline 225 (2007) compliant to GLP (reliability category 1). Test duration was 28 days after insertion of the test organisms. A limit test with the nominal test concentration of 1000 mg/kg sediment dry weight (DW) was performed by spiking the test item into the sediment. Six replicates per control, vehicle control and limit test item concentration with ten worms per replicate were set up. Water quality parameters as temperature, pH-value, O2-concentration, ammonia and total hardness were determined.
Due to the chemical properties of the test item the fraction of the pigment in the overlying water
as well as in the porewater can be neglected. Thus, no analytical verification of the test item
was performed.
After 28 days no mortality or significant behavioural changes of L. variegatus were observed at the limit test item concentration of 1000 mg/kg sediment DW. Also, the biomass was not significantly reduced compared to the pooled control after 28 days of exposure to the test item. L. variegatus reproduced without any significant differences between the pooled control and the limit test item concentration of 1000 mg/kg sediment DW (Table 1).
Table 1: Summary of all Significant Effects
Effects
Test item concentration
Significance of effects
[mg/kg sediment DW]
Mortality
> 1000
Behaviour
> 1000
Biomass
> 1000
Reproduction
> 1000
NOEC
(mortality, behaviour, biomass, reproduction)
>= 1000
- Endpoint:
- sediment toxicity: long-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2008-05-26 to 2008-06-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study compliant to GLP
- Justification for type of information:
- See Rationale and Justification for the Analogue Read-Across Approach for the registration of the Nanoform of Pigment Red 188 using Pigment Red 22, Pigment Red 112 and Pigment Orange 38 as Source Substances (Chapter 13)
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 225: Sediment-Water Lumbriculus Toxicity Test Using Spiked Sediment (October 2007)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- GLP according to German Chemikaliengesetz and Directive 88/320/EEC
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on sediment and application:
- PREPARATION OF SPIKED SEDIMENT
- Details of spiking: The test substance concentration was weighed out, dissolved in 15 mL acetone and mixed thoroughly with 60 g quartz sand (10 g quartz sand per replicate) in a glass vessel. After complete evaporation of the solvent, the dry quartz sand was given to the sediment. Residues of the dry quartz sand sticking at the glas vessel were rinsed off with 50 mL demineralised water per treatment. Thereafter, the sediment was thoroughly mixed with a long handled blender for at least three minutes and filled into the test vessels.
- Equilibration time: 2 days prior to insertion of test organisms
- Equilibration conditions: Same as test
- Controls: Controls with test sediment and quartz sand. Vehicle controls with test sediment and quartz sand treated with acetone.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): organic solvent, acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 15 mL per 60 g quartz sand (6 replicates)
- Evaporation of vehicle before use: Yes, complete evaporation
- Test organisms (species):
- Lumbriculus variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: Lumbriculus variegatus (Müller), Lumbriculidae, Oligochaeta
- Source: Bundesanstalt für Gewässerkunde (BfG), Am Mainzer Tor 1, 56068 Koblenz, Germany
- Age of test organisms (mean and range, SD): Synchronized from cultured organisms 13 days before test insertion
- Breeding conditions: 20 +/- 2 deg C, diffuse light (100 - 1000 lx, 16 h photoperiod) dissolved oxygen concentration > 60 % of the air saturation value
- Handling of worms: 13 days before insertion into the test, the worms were synchronized. Therefore, a sufficient amount of worms was taken from the breeding and cut into half.. After regeneration of heads, the worms were inserted into the test.
- Age of animals at beginning of exposure: 13 days after synchronisation, completely regenerated head region. Synchronization was done to minimize uncontrolled regeneration and reproduction during the test.
- Feeding during test
- Food type: Powder of Urtica dioica (Heinrich Klein GmbH & Co KG, 97525 Schwebheim, Germany)
- Amount: 0.5 % Urtica powder
- Frequency: Urtica powder was mixed into the sediment once at test start.
ACCLIMATION
- Acclimation period: 13 days (after synchronization)
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Same as test
- Feeding frequency: Same as test (Once)
- Health during acclimation (any mortality observed): No mortality observed - Study type:
- laboratory study
- Test type:
- static
- Water media type:
- freshwater
- Type of sediment:
- artificial sediment
- Limit test:
- yes
- Duration:
- 28 d
- Exposure phase:
- total exposure duration
- Hardness:
- Day 0: Control: > 356 mg/L CaCO3, Solvent Control: 340 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3
Day 28: Control: > 356 mg/L CaCO3, Solvent Control: > 356 mg/L CaCO3, 1000 mg test substance/kg sediment DW: > 356 mg/L CaCO3 - Test temperature:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 Temperature [°C] 21.1 21.0 20.9
7 Temperature [°C] 21.9 21.2 21.9
14 Temperature [°C] 22.0 20.2 20.2
21 Temperature [°C] 21.8 21.8 21.4
28 Temperature [°C] 21.8 21.2 20.8 - pH:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 pH 7.74 7.82 7.88
7
pH 7.86 7.86 7.88
14
pH 7.76 7.79 7.86
21
pH 7.58 7.87 8.01
28
pH 8.22 8.27 8.29 - Dissolved oxygen:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 O2 [mg/L] 6.16 6.78 6.37
7 O2 [mg/L] 6.06 6.05 6.40
14 O2 [mg/L] 6.90 6.22 6.71
21 O2 [mg/L] 7.37 7.11 7.05
28
O2 [mg/L] 7.30 8.04 7.96 - Salinity:
- Not measured, freshwater conditions
- Ammonia:
- Day Parameter Treatment
Control Solvent Control 1000 mg/kg sediment DW
0 NH4-N [mg/L] 0.803 0.622 0.797
2 NH4-N [mg/L] 0.114 < 0.02* < 0.02*
5 NH4-N [mg/L] 0.039 0.031 0.113
8 NH4-N [mg/L] 0.045 0.039 0.044
9 NH4-N [mg/L] 0.091 0.050 0.031
12 NH4-N [mg/L] 0.064 0.060 0.068
15 NH4-N [mg/L] 0.089 0.241 0.063
16 NH4-N [mg/L] 1.03 0.715 0.121
19 NH4-N [mg/L] > 2.5* > 2.5* > 2.5*
NH4-N [mg/L] 5.71 5.35 4.29
23 NH4-N [mg/L] 4.84 4.75 5.42
26 NH4-N [mg/L] 6.91 6.01 5.85
28 NH4-N [mg/L] 6.76 6.58 4.30
- Nominal and measured concentrations:
- ominal concentration limit concentration: 1000 mg test substance/kg sediment DW.
The submission substance has a low water solubility and due to its sorption to particular matter, low volatility and very low biotic and abiotic degradation. It resides predominantly in the solid phase of the sediment. The fraction of the pigment in the overlying water as well as the porewater can be neglected. As no loss of pigment was expected to occur during the exposure period an analytical determination of the pigment in the overlying water and the sediment has been omitted. Based on the fact that the test system consists of 150 mL overlying water 0.008 % pigment would be in the overlying water at saturation and thus 99.992 % remains in the sediment (solids & porewater). - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 250 mL glass beakers, 6 cm diameter
- Sediment volume: 45 g DW per vessel, ca. 2 cm from the ground
- Overlying water volume: 150 mL
- Depth of sediment and overlying water: Sediment depth ca. 2 cm, water depth ca. 10 cm
- Aeration: yes
- Aeration frequency and intensity: Gentle aeration (2 bubbles per second)
- Replacement of evaporated test water, if any: Yes, once weekly
EXPOSURE REGIME
- No. of organisms per container (treatment): 10 per container (60 per treatment)
- No. of replicates per treatment group: 6 replicates per treatment group
- No. of replicates per control / vehicle control: 6 replicates per control/vehicle control
- Feeding regime: Once at test start
- Type and preparation of food: Powder of Urtica dioica mixed into the sediment before insertion of test organisms
- Amount of food: 0.5 %
OVERLYING WATER CHARACTERISTCS
- Type of water (e.g. deionized, ground water, sea water, Elendt medium acc. to OECD 219): Reconstituted water acc. to OECD 203
CHARACTERIZATION OF (ARTIFICIAL; delete if not applicable) SEDIMENT
- Composition (if artificial substrate): 5 % peat air dried and finely ground, 20 % kaolin, 75 % quartz sand, 0.33 % calcium carbonate
Table 1: Sediment Properties at Test Start
Total Organic Carbon [%] pH-value Moisture [%]
Sediment at test start 2.15 6.74 8.29
OTHER TEST CONDITIONS
Room temperature [°C] Photoperiod [h] Light intensity [lx]
Nominal 20 +/- 2 16 100 - 1000
Actual 18 - 21 16 472 +/- 47.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and behaviour changes (Day 0-28), reproduction and biomass at test end (28 d)
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
1000 mg test substance/kg sediment DW
- Range finding study
- Test concentrations: 10 - 100 - 1000 mg/kg sediment DW
- Results used to determine the conditions for the definitive study:
Concentration Mortality Mean number of worms Mean dry weight
[mg test item/kg sediment DW] [%] [mg]
Control 0 13.5 14.7
Solv. Control 0 14.5 16.0
10 0 13.0 35.4
100 0 11.5 16.8
1000 0 12.0 15.9 - Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality, behaviour, biomass reproduction
- Details on results:
- - Mortality of test animals at end of exposure period: No mortality
- Total mass of test animals at beginning of test: 10 worms were equal to ca. 45.8 mg wet mass
- Morphological abnormalities: None
- Behavioural abnormalities: None - Reported statistics and error estimates:
- One Way Analysis of Variance (ANOVA, Dunnett´s Method) was carried out with worm numbers and biomass data for the determination of statistically significant differences compared to the pooled control. Prior to the ANOVA a normality test and an equal variance test were conducted. P-values for both normality and equal variance test were 0.05. The alpha-value (acceptable probability of incorrectly concluding that there is a difference) for ANOVA was alpha = 0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this sedmient-water Lubriculus toxicity test according to OECD 225 the submission substance caused no significant effects on mortality, behaviour, biomass and reproduction at the limit test item concentration of 1000 mg/kg sediment DW. Thus, the NOEC was determined to be equal or larger than 1000 mg/kg sediment DW.
- Executive summary:
The effects of the test item on the oligochaete Lumbriculus variegatus in a water-sediment system were determined. The study was carried out according to OECD Guideline 225 (2007) compliant to GLP (reliability category 1). Test duration was 28 days after insertion of the test organisms. A limit test with the nominal test concentration of 1000 mg/kg sediment dry weight (DW) was performed by spiking the test item into the sediment. Six replicates per control, vehicle control and limit test item concentration with ten worms per replicate were set up. Water quality parameters as temperature, pH-value, O2-concentration, ammonia and total hardness were determined.
Due to the chemical properties of the test item the fraction of the pigment in the overlying water
as well as in the porewater can be neglected. Thus, no analytical verification of the test item
was performed.
After 28 days no mortality or significant behavioural changes of L. variegatus were observed at the limit test item concentration of 1000 mg/kg sediment DW. Also, the biomass was not significantly reduced compared to the pooled control after 28 days of exposure to the test item. L. variegatus reproduced without any significant differences between the pooled control and the limit test item concentration of 1000 mg/kg sediment DW (Table 1).
Table 1: Summary of all Significant Effects
Effects
Test item concentration
Significance of effects
[mg/kg sediment DW]
Mortality
> 1000
Behaviour
> 1000
Biomass
> 1000
Reproduction
> 1000
NOEC
(mortality, behaviour, biomass, reproduction)
>= 1000
Referenceopen allclose all
Mortality/Reproduction of worms:
After 28 days of exposure to the limit test item concentration the total number of worms did not differ significantly compared to the pooled control (Table 1). No mortality of complete worms was observed due to the exposure to the limit test item concentration of 1000 mg/kg sediment DW over a period of 28 days (Table 2). Also, the number of incomplete worms and thus the reproduction did not significantly differ to the pooled control at test end (Table 3).
Table 1: Total Worm Number after 28 days of Exposure
Nominal conc. |
Total Number of |
|||||||||
Day 28 |
||||||||||
[mg/kg sediment DW] |
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Inhibition |
Significance* |
Control |
17 |
19 |
31 |
20 |
23 |
22 |
22.9 |
4.76 |
- |
- |
Solvent Control |
21 |
32 |
25 |
26 |
19 |
20 |
||||
1000 |
20 |
20 |
22 |
20 |
22 |
18 |
20.3 |
1.51 |
11.3 |
No |
Table 2: Mortality after 28 days of Exposure
Nominal conc. [mg/kg sediment DW] |
Total Number of Complete |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Mortality |
Significance |
|
Control |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
0 |
0 |
- |
Solvent Control |
10 |
10 |
10 |
10 |
10 |
10 |
||||
1000 |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
0 |
0 |
No |
Table 3: Reproduction after 28 days of Exposure
Nominal conc. [mg/kg sediment DW] |
Number of incomplete worms |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Inhibition |
Significance* |
|
Control |
7 |
9 |
21 |
10 |
13 |
12 |
12.9 |
4.76 |
- |
|
Solvent Control |
11 |
22 |
15 |
16 |
9 |
10 |
||||
1000 |
10 |
10 |
12 |
10 |
12 |
8 |
10.3 |
1.51 |
20.0 |
No |
No difference in the worm behaviour was observed at the limit test item concentration of 1000 mg/kg sediment DW compared to the pooled control during the entire study (Table 4).
Table 4: Worm Behaviour during 28 Days of Exposure
Nominal conc. [mg/kg sediment DW] |
Effect |
Days after insertion (pooled replicates) |
||||||||||||
0 |
2 |
5 |
7 |
9 |
12 |
14 |
16 |
19 |
21 |
23 |
26 |
28 |
||
Control |
s) |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
Solvent Control |
s) |
60 |
60 |
58 |
60 |
60 |
60 |
60 |
59 |
60 |
60 |
60 |
60 |
60 |
a) |
0 |
0 |
2 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
|
1000 |
s) |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
conc. = concentration DW = Dry Weight
s) = worm burrowing in sediment a) = worm on sediment surface t) = dead
Dry biomass of the worms
The dry biomass of the worms at the limit test item concentration of 1000 mg/kg sediment DW did not differ significantly to the pooled control (Table 5).
Table 5: Worm Dry Biomass after 28 Days of Exposure
Nominal conc. [mg/kg sediment DW] |
Dry weight [mg] |
||||||||
Replicate |
per Repl. |
Significance* |
per worm |
||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV ± SD |
MV ± SD |
||
Control |
7.10 |
12.7 |
18.2 |
14.0 |
13.5 |
13.0 |
14.8 ± 3.27 |
- |
0.65 ± 0.12 |
Solvent Control |
15.5 |
20.3 |
14.9 |
15.7 |
16.7 |
16.0 |
|||
1000 |
13.7 |
14.4 |
19.0 |
12.3 |
16.8 |
13.3 |
14.9 ± 2.51 |
No |
0.73 ± 0.08 |
Mortality/Reproduction of worms:
After 28 days of exposure to the limit test item concentration the total number of worms did not differ significantly compared to the pooled control (Table 1). No mortality of complete worms was observed due to the exposure to the limit test item concentration of 1000 mg/kg sediment DW over a period of 28 days (Table 2). Also, the number of incomplete worms and thus the reproduction did not significantly differ to the pooled control at test end (Table 3).
Table 1: Total Worm Number after 28 days of Exposure
Nominal conc. |
Total Number of |
|||||||||
Day 28 |
||||||||||
[mg/kg sediment DW] |
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Inhibition |
Significance* |
Control |
17 |
19 |
31 |
20 |
23 |
22 |
22.9 |
4.76 |
- |
- |
Solvent Control |
21 |
32 |
25 |
26 |
19 |
20 |
||||
1000 |
20 |
20 |
22 |
20 |
22 |
18 |
20.3 |
1.51 |
11.3 |
No |
Table 2: Mortality after 28 days of Exposure
Nominal conc. [mg/kg sediment DW] |
Total Number of Complete |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Mortality |
Significance |
|
Control |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
0 |
0 |
- |
Solvent Control |
10 |
10 |
10 |
10 |
10 |
10 |
||||
1000 |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
0 |
0 |
No |
Table 3: Reproduction after 28 days of Exposure
Nominal conc. [mg/kg sediment DW] |
Number of incomplete worms |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Inhibition |
Significance* |
|
Control |
7 |
9 |
21 |
10 |
13 |
12 |
12.9 |
4.76 |
- |
|
Solvent Control |
11 |
22 |
15 |
16 |
9 |
10 |
||||
1000 |
10 |
10 |
12 |
10 |
12 |
8 |
10.3 |
1.51 |
20.0 |
No |
No difference in the worm behaviour was observed at the limit test item concentration of 1000 mg/kg sediment DW compared to the pooled control during the entire study (Table 4).
Table 4: Worm Behaviour during 28 Days of Exposure
Nominal conc. [mg/kg sediment DW] |
Effect |
Days after insertion (pooled replicates) |
||||||||||||
0 |
2 |
5 |
7 |
9 |
12 |
14 |
16 |
19 |
21 |
23 |
26 |
28 |
||
Control |
s) |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
Solvent Control |
s) |
60 |
60 |
58 |
60 |
60 |
60 |
60 |
59 |
60 |
60 |
60 |
60 |
60 |
a) |
0 |
0 |
2 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
|
1000 |
s) |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
conc. = concentration DW = Dry Weight
s) = worm burrowing in sediment a) = worm on sediment surface t) = dead
Dry biomass of the worms
The dry biomass of the worms at the limit test item concentration of 1000 mg/kg sediment DW did not differ significantly to the pooled control (Table 5).
Table 5: Worm Dry Biomass after 28 Days of Exposure
Nominal conc. [mg/kg sediment DW] |
Dry weight [mg] |
||||||||
Replicate |
per Repl. |
Significance* |
per worm |
||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV ± SD |
MV ± SD |
||
Control |
7.10 |
12.7 |
18.2 |
14.0 |
13.5 |
13.0 |
14.8 ± 3.27 |
- |
0.65 ± 0.12 |
Solvent Control |
15.5 |
20.3 |
14.9 |
15.7 |
16.7 |
16.0 |
|||
1000 |
13.7 |
14.4 |
19.0 |
12.3 |
16.8 |
13.3 |
14.9 ± 2.51 |
No |
0.73 ± 0.08 |
Mortality/Reproduction of worms:
After 28 days of exposure to the limit test item concentration the total number of worms did not differ significantly compared to the pooled control (Table 1). No mortality of complete worms was observed due to the exposure to the limit test item concentration of 1000 mg/kg sediment DW over a period of 28 days (Table 2). Also, the number of incomplete worms and thus the reproduction did not significantly differ to the pooled control at test end (Table 3).
Table 1: Total Worm Number after 28 days of Exposure
Nominal conc. |
Total Number of |
|||||||||
Day 28 |
||||||||||
[mg/kg sediment DW] |
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Inhibition |
Significance* |
Control |
17 |
19 |
31 |
20 |
23 |
22 |
22.9 |
4.76 |
- |
- |
Solvent Control |
21 |
32 |
25 |
26 |
19 |
20 |
||||
1000 |
20 |
20 |
22 |
20 |
22 |
18 |
20.3 |
1.51 |
11.3 |
No |
Table 2: Mortality after 28 days of Exposure
Nominal conc. [mg/kg sediment DW] |
Total Number of Complete |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Mortality |
Significance |
|
Control |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
0 |
0 |
- |
Solvent Control |
10 |
10 |
10 |
10 |
10 |
10 |
||||
1000 |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
0 |
0 |
No |
Table 3: Reproduction after 28 days of Exposure
Nominal conc. [mg/kg sediment DW] |
Number of incomplete worms |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV |
SD |
% Inhibition |
Significance* |
|
Control |
7 |
9 |
21 |
10 |
13 |
12 |
12.9 |
4.76 |
- |
|
Solvent Control |
11 |
22 |
15 |
16 |
9 |
10 |
||||
1000 |
10 |
10 |
12 |
10 |
12 |
8 |
10.3 |
1.51 |
20.0 |
No |
No difference in the worm behaviour was observed at the limit test item concentration of 1000 mg/kg sediment DW compared to the pooled control during the entire study (Table 4).
Table 4: Worm Behaviour during 28 Days of Exposure
Nominal conc. [mg/kg sediment DW] |
Effect |
Days after insertion (pooled replicates) |
||||||||||||
0 |
2 |
5 |
7 |
9 |
12 |
14 |
16 |
19 |
21 |
23 |
26 |
28 |
||
Control |
s) |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
Solvent Control |
s) |
60 |
60 |
58 |
60 |
60 |
60 |
60 |
59 |
60 |
60 |
60 |
60 |
60 |
a) |
0 |
0 |
2 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
|
1000 |
s) |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
60 |
conc. = concentration DW = Dry Weight
s) = worm burrowing in sediment a) = worm on sediment surface t) = dead
Dry biomass of the worms
The dry biomass of the worms at the limit test item concentration of 1000 mg/kg sediment DW did not differ significantly to the pooled control (Table 5).
Table 5: Worm Dry Biomass after 28 Days of Exposure
Nominal conc. [mg/kg sediment DW] |
Dry weight [mg] |
||||||||
Replicate |
per Repl. |
Significance* |
per worm |
||||||
1 |
2 |
3 |
4 |
5 |
6 |
MV ± SD |
MV ± SD |
||
Control |
7.10 |
12.7 |
18.2 |
14.0 |
13.5 |
13.0 |
14.8 ± 3.27 |
- |
0.65 ± 0.12 |
Solvent Control |
15.5 |
20.3 |
14.9 |
15.7 |
16.7 |
16.0 |
|||
1000 |
13.7 |
14.4 |
19.0 |
12.3 |
16.8 |
13.3 |
14.9 ± 2.51 |
No |
0.73 ± 0.08 |
Description of key information
PO038
In this sediment-water Lubriculus toxicity test according to OECD 225 the test substance caused no significant effects on mortality, behaviour, biomass and reproduction at the limit test item concentration of 1000 mg/kg sediment DW. Thus, the NOEC was determined to be equal or larger than 1000 mg/kg sediment DW.
Key value for chemical safety assessment
- EC10, LC10 or NOEC for freshwater sediment:
- 1 000 mg/kg sediment dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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