Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 618-460-1 | CAS number: 9010-89-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vitro / ex vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant non-guideline study, conducted with read-across substance
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
- Objective of study:
- other: hydrolysis in rat plasma
Test guideline
- Qualifier:
- no guideline required
- Principles of method if other than guideline:
- Investigation of the stability of Adipic acid, cyclic ester with diethyleneglycol in phosphate buffer, hydrochloric acid (pH 3.0), and in rat plasma.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,3,7,8-tetrahydro-2,5,8-benzotrioxacycloundecine-1,9-dione
- EC Number:
- 229-554-3
- EC Name:
- 2,3,7,8-tetrahydro-2,5,8-benzotrioxacycloundecine-1,9-dione
- Cas Number:
- 6607-34-7
- Molecular formula:
- C10H16O5
- IUPAC Name:
- 1,4,7-Trioxacyclotridecane-8,13-dione
- Reference substance name:
- 1,4,7 Trioxacylotridecane - 8, 13 dione
- IUPAC Name:
- 1,4,7 Trioxacylotridecane - 8, 13 dione
- Details on test material:
- Adipic acid, cyclic ester with diethyleneglycol
Purity >90%
Test substance No.: 99/147-1
Appearence: white solid
Constituent 1
Constituent 2
Administration / exposure
- Duration and frequency of treatment / exposure:
- Sampling of the incubates 0, 1, 2 and 4 h after application of the test substance.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1 mg/ml in phosphate buffer (50 mM Na-phosphate, pH 7.1) and hydrochloric acid (pH 3.0) (nominal)
1 and 5 mg/ml in rat plasma (nominal)
- Details on study design:
- Plasma was prepared from two male Wistar rats.
All incubations were performed at 37°C.
Samples of the incubates were taken immediatelly after addition of Adipic acid, cyclic ester with diethyleneglycol (0 h) and after 1, 2 and 4 h.
1) Investigation of non-enzymatic hydrolysis in plasma:
stability of Adipic acid, cyclic ester with diethyleneglycol in phosphate buffer (50 mM Na-phosphate, pH 7.1) at a nominal concentration of 1 mg/ml.
2) Investigation of hydrolysis in stomach:
stability of Adipic acid, cyclic ester with diethyleneglycol in hydrochloric acid (pH 3.0) at a nominal concentration of 1 mg/ml.
3) Investigation of the hydrolysis of Adipic acid, cyclic ester with diethyleneglycol in freshly prepared rat plasma, nominal concentrations were 1 and 5 mg/ml.
Ahead of analysis the protein was precipitated by ZnSO4-solution and subsequently centrifuged.
Analytical determination of either the test substance itself or by formation of the hydrolytic product adipic acid by HPLC. Quantification in the samples was done by external calibration using stock solutions in aqua bidest.
Results and discussion
Any other information on results incl. tables
The limit of detection of adipic acid was about 4 µg/ml (27 nmol/ml; 27 µM). This means that hydrolysis of only 1% of Adipic acid, cyclic ester with diethyleneglycol could be detected with this method.
1)-2) After a 4h incubation of Adipic acid, cyclic ester with diethyleneglycol in phosphate buffer or hydrochloric acid, no hydrolysis could be detected . Therefore a non-enzymatic hydrolysis either at neutral pH or at a low pH-value simulating conditions in the stomach cannot be anticipated.
3) Hydrolysis of Adipic acid, cyclic ester with diethyleneglycol with corresponding formation of adipic acid could be demonstrated at both concentrations tested. The hydrolysed portion increased with the duration of the incubation and the concentration used.
Table: Enzymatic hydrolysis of Adipic acid, cyclic ester with diethyleneglycol in rat plasma.
Nominal conc. [µg/ml] |
Time [h] |
Mean adipic acid [µg/ml]; n=2 |
Corresponding % adipic acid, cyclic ester with diethyleneglycol hydrolysed |
1068 |
0 |
21.5 |
2.98 |
|
1 |
99.8 |
13.81 |
|
2 |
119.4 |
16.52 |
|
4 |
131.4 |
18.18 |
5335 |
0 |
73.1 |
2.02 |
|
1 |
1552.5 |
43 |
|
2 |
2224.6 |
62.62 |
|
4 |
3268.3 |
90.53 |
In control experiments it could be shown that Adipic acid, cyclic ester with diethyleneglycol was considerably bound to plasma proteins. The fact that enzymatic ester hydrolysis is more pronounced at higher concentrations may therefore result from saturation of protein binding and a consequently higher free plasma concentration.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.