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EC number: 247-269-2 | CAS number: 25797-81-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Remarks:
- BOD
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)
- Deviations:
- yes
- Remarks:
- (aerate over night after addition of the inoculum)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Code number: FAT - 20'033/H
EN/Batch number: 672001.29
Purity: 72 %
Appearance: Solid
Solubility: 80 g/L (in water)
Storage: room temperature
Expiration date: January 1984 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Seeding water of an urban waste water
- Details on inoculum:
- - Source of inoculum/activated sludge: Seeding water of an urban waste water
- Duration of test (contact time):
- 5 d
- Initial conc.:
- 6.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12.5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 25.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 50.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 100.3 mg/L
- Based on:
- test mat.
- Initial conc.:
- 200.6 mg/L
- Based on:
- test mat.
- Initial conc.:
- 401.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 802.2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Temperature: 20 ± 1 °C
Duration: 5 d
Estimation Technique: O2 determination, measured with an oxygen sensitive electrode system: SYLAND (SAV: 00 107)
Suppression of nitrification: without suppression of nitrification - Reference substance:
- other: D(+)-Glucose /L-Glutamic acid
- Remarks on result:
- not measured/tested
- Details on results:
- The BOD5 of the test substance corrected by the blank control is 0 mg O2/g. Further, the COD value was taken from an another experiment and was found to be 1416 mg O2/L. The % biodegradation was calculated using the below formula: BOD/COD*100.
Hence, the biodegradation of the test substance is 0 %. - Parameter:
- BOD5
- Value:
- 0 mg O2/g test mat.
- Results with reference substance:
- The BOD5 of glucose/glutamic acid solution is 205 mg O2/L.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: BOD5 determined
- Conclusions:
- The BOD5 of the test substance corrected by the blank control was 0 mg O2/g.
- Executive summary:
The biochemical oxygen demand of the test substance (of ca. 72 % purity) was determined by measuring the dissolved oxygen concentration before and after 5 d incubation at 20 °C at different concentrations diluted in water saturated with dissolved oxygen and containing a seed of microorganisms. The test was carried out according to ISO 5815 in compliance with GLP. The substance was tested at ca. 6 to 800 mg/L. The reference substance D(+)-Glucose / L-Glutamic acid was tested at a concentration of 20 mL/L. Under the study conditions, the BOD5 of the test substance corrected by the blank control was 0 mg O2/g. Further, the COD value from another experiment was found to be 1416 mg O2/L. The % biodegradation was calculated using the formula: BOD/COD*100. Hence, the biodegradation of the test substance was 0 %.
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- Chemical Oxygen Demand
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.9 (Biodegradation: Zahn-Wellens Test)
- Deviations:
- no
- Principles of method if other than guideline:
- A predetermined amount of the test substance dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 h.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Code number: FAT - 20'033/H
EN/Batch number: 672001.29
Purity: 72 %
Appearance: Solid
Solubility: 80 g/L (in water)
Storage: room temperature
Expiration date: January 1984 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- not specified
- Duration of test (contact time):
- 2 h
- Initial conc.:
- 2.5 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Sample weight: 2.5 mg of the test substance
Temperature: 148 ± 3 °C
Duration: 2 h
Estimation Technique: Titration (Potentiometric), METROHM Titroprozessor 670 (SAV : 00 111) - Reference substance:
- other: Potassium hydrogen phthalate solution
- Remarks on result:
- not measured/tested
- Details on results:
- none
- Parameter:
- COD
- Value:
- 1 416 mg O2/g test mat.
- Results with reference substance:
- The COD of the reference substance is 198 mg O2/L (Criteria: 200 ± 8 mg O2/L)
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: COD determined
- Conclusions:
- The COD of the test substance was found to be 1416 mg O2/g.
- Executive summary:
COD of FAT 2033/G was estimated according to method EEC Directive 94/449 (1984) Part C.9 (DEV H 41 -1) under GLP. A predetermined amount of test substance (of ca. 72 % purity) dissolved in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 h. The residual dichromate was determined by titration with standardized ferrous ammonium sulphate. The substance was tested at 2.5 mg/L. Under the study conditions, the COD of the test substance was 1416 mg O2/g. Further, the BOD5 value from another experiment was found to be 0 mg O2/L. The % biodegradation was calculated using the formula: BOD/COD*100. Hence, the biodegradation of the test substance was 0 %.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO Method 8192 B
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Code number: FAT - 20033/H
EN/Batch number: 672001.29
Purity: 72 %
Appearance: Solid
Solubility: 80 g/L (in water)
Storage: room temperature
Expiration date: January 1984 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Sludge of a biological sewage treatment plant (ARA-Therwil) collected on 22.06.1993
- Amount: 1020 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 150 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Water/nutrient: According to the Guideline
Temperature: 22.0 ± 3 °C (room temperature)
Test duration: 28 d
Lighting: Indirect daylight
Estimation technique: TOC/DOC analyzer / SHIMADZU TOC-500 - Reference substance:
- diethylene glycol
- Preliminary study:
- The mean value of preliminary test is 146.4 mg/L.
- Test performance:
- no data
- Parameter:
- % degradation (DOC removal)
- Value:
- 3.1
- Sampling time:
- 28 d
- Details on results:
- The bioelimination of the test substance measured as DOC after 28 d corrected by the blank control was 3.1 % (average of 2 tests running in parallel). Further, the adsorption after 3 h was 2.9 %.
- Results with reference substance:
- The bioelimination, corrected by the blank control and measured as DOC (mg/L) is 98.7 % 16th day.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The test substance was not considered to be inherently biodegradable.
- Executive summary:
The inherent biodegradability of the test substance (of ca. 72 % purity) was determined in a 28 d Zahn-Wellens test according to OECD Guideline 302B and ISO Method 8192 B in compliance with GLP. The substance was tested at 150 mg/L DOC. The bioelimination of the test substance measured as DOC corrected by the blank control was 3.1 % (average of 2 tests running in parallel). Further, the adsorption after 3 h was 2.9 %. Under the study conditions, the test substance was not considered to be inherently biodegradable.
Referenceopen allclose all
BOD values of the oxygen measurements:
Substance |
Test-concentration E = mg/L |
Start mg O2/L T0 |
End mg O2/L T5 |
Blank mg O2/L B1 |
mg O2/g BOD5 |
Test substance |
802.2 |
8.8 8.8 |
8.3 8.5 |
0.4 0.4 |
0 0 |
401.1 |
8.8 8.8 |
8.4 8.5 |
0.4 0.4 |
0 0 |
|
200.6 |
8.8 8.7 |
8.4 8.4 |
0.4 0.4 |
0 0 |
|
100.3 |
8.8 8.7 |
8.4 8.5 |
0.4 0.4 |
0 0 |
|
50.1 |
8.7 8.7 |
8.1 8.3 |
0.4 0.4 |
4 0 |
|
25.1 |
8.7 8.7 |
8.3 8.4 |
0.4 0.4 |
0 0 |
|
12.5 |
8.7 8.7 |
8.3 8.4 |
0.4 0.4 |
0 0 |
|
6.5 |
8.7 8.7 |
8.3 8.4 |
0.4 0.4 |
0 0 |
COD analytical data:
Product |
Sample weight E(mg) |
Titration (mL) |
N = |
mg O2/ g |
||
|
|
a |
b |
c |
|
|
Normality |
|
|
|
10.100 10.103 |
0.1188 0.1187 Ǿ : 0.1187 |
|
Blank |
|
9.910 9.900 Ǿ : 9.905 |
|
|
|
|
Test substance |
2.5 mg 2.5 mg |
|
6.157 6.199 |
|
|
1424 1408 Ǿ :1416 |
Table:
|
| Reference | Substance | ||||
Timepoint | Date | Mean Value of the Reference DOC (mg/l)
| Value of the Blank DOC (mg/l)
| Elimination % | Mean Value of the Substance DOC (mg/l) | Value of the Blank DOC (mg/l)
| Elimination % |
-1 day | 22.06.93 | 151.9 | 7.3 | - | 146.4 | - | - |
0 | 23.06.93 | 159.3 | 12.4 | 0 | 151.9 | 7.3 | 1.2 |
3 h | 23.06.93 | 163.3 | 12.8 | 0.7 | 154.5 | 12.4 | 2.9 |
2 day | 25.06.93 | 164.4 | 22.5 | 0.2 | 152.5 | 12.8 | 4.6 |
5 day | 28.06.93 | 170.1 | 21.7 | 2.8 | 143.3 | 22.5 | 17.5 |
7 day | 30.06.93 | 170.1 | 17.4 | 2.3 | 145.7 | 21.7 | 15.3 |
9 day | 02.07.93 | 161.1 | 13.0 | 5.4 | 142.4 | 17.4 | 14.6 |
12 day | 05.07.93 | 14.3 | 12.9 | 99.1 | 134.9 | 13.0 | 16.7 |
13 day | 06.07.93 | 13.8 | 11.3 | 99.4 | - | - | - |
14 day | 07.07.93 | 13.4 | 8.4 | 98.6 | 133.7 | 11.3 | 16.4 |
15 day | 08.07.93 | 11.8 | 11.0 | 97.8 | - | - | - |
16 day | 09.07.93 | 13.0 | 10.8 | 98.7 | 130.4 | 11.0 | 18.4 |
19 day | 12.07.93 | - | 6.4 | - | 129.3 | 10.8 | 19.1 |
21 day | 14.07.93 | - | 12.7 | - | 125.9 | 6.4 | 18.4 |
23 day | 16.07.93 | - | 9.2 | - | 146.4 |
| 12.0 |
26 day | 19.07.93 | - | 8.9 | - | 151.9 |
| 1.4 |
28 day | 21.07.93 | - | 7.3 | - | 154.5 |
| 3.1 |
Description of key information
Acid Blue 277 is considered to be not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The inherent biodegradability of FAT 20033/H (of ca. 72 % purity) was determined in a 28-days Zahn-Wellens test according to OECD Guideline 302B and ISO Method 8192 B in compliance with GLP. The substance was tested at 150 mg/L DOC. The bioelimination of the test substance measured as DOC corrected by the blank control was 3.1 % (average of 2 tests running in parallel). Further, the adsorption after 3 h was 2.9 %. Under the study conditions, the test substance was not considered to be inherently biodegradable.
The BOD5 of FAT 20033/H corrected by the blank control was 0 mg O2/g. Further, the COD value from another experiment was found to be 1416 mg O2/L. The biodegradation [%] was calculated using the formula: BOD/COD*100. Hence, the biodegradation of the test substance was 0 %.
Several supporting studies investigating Biological Oxygen Demand (BOD5), Chemical Oxygen Demand (COD) and inherent biodegradability of Acid Blue 277 are available. The weight of evidence from these studies supports the conclusion that Acid Blue 277 is not readily biodegradable.
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