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EC number: 606-524-1 | CAS number: 203313-47-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- adopted July, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- methyl cis-1-{[(2,5-dimethylphenyl)acetyl]amino}-4-methoxycyclohexanecarboxylate
- EC Number:
- 606-524-1
- Cas Number:
- 203313-47-7
- Molecular formula:
- C19H27NO4
- IUPAC Name:
- methyl cis-1-{[(2,5-dimethylphenyl)acetyl]amino}-4-methoxycyclohexanecarboxylate
- Test material form:
- solid: bulk
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Aeration tank of a wastewater plant treating predominantly domestic sewage
(Wupper area water authority, STP Odenthal)
- Laboratory culture: the sludge was washed twice by adding mineral medium and centrifuging
for 10 min at 2000 rpm and 20 °C and decanting off the supernatant
- Storage conditions: The inoculum was stored at room temperature under continuous shaking
with aeration until use for max. 3 days
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22°C
- pH: 7.4 - 7.9
- pH adjusted: no
TEST SYSTEM
- Culturing apparatus: Oxitop
- Number of culture flasks/concentration: 2
- Test performed in closed vessels due to significant volatility of test substance:
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3
- Abiotic sterile control: -
- Toxicity control: 1
Pre-treatment of the test item
25 mg of the test item were weighed out on aluminium foil. Test item and
aluminium foil were added to the test flasks, which had already been filled with 200
mL of mineral medium. Afterwards the flask content was made up to 250 mL with
mineral medium containing inoculum to give a test concentration of 100 mg test
item/L.
Exposure conditions
Test volume: 250 mL
Test apparatus: OxiTop System (WTW)
Mixing: 1 magnetic stirrer per test vessel
Incubation time: 28 days
Incubation temperature: 22±1 °C
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1.4
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0.4
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 82.0 % in 14 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Remarks:
- Within 28 days, a degradation o 0 % was determined.
- Conclusions:
- 1.0 % degradation after 7 days
0.4 % degradation after 14 days
0 % degradation after 21 days
0 % degradation after 28 days
Therefore, the test subsance is considered to be “Not Readily Biodegradable“. - Executive summary:
The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D “Manometric Respirometry Test“(1992). This test method is in all essential parts identical with OECD Guideline 301 F (adopted July 1992).
Within 28 days, a degradation of 0 % was determined for the test substance.
The test substance is considered to be "Not Readily Biodegradable".
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