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EC number: 237-424-2 | CAS number: 13780-06-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13-15 April 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Good-quality guideline study, conducted to GLP. Certain water quality parameters (e.g. hardness, conductivity) were not reported.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Produced by BASF on 23 November 2009. 47219706D0 vom 23.11.2009
- Expiration date of the lot/batch: November 2012
- Purity test date: not specified
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions: "The stability of the test substance under storage conditions over the test period was guaranteed by the sponsor and the sponsor holds this responsibility".
- Solubility and stability of the test substance in the solvent/vehicle: water solubility 848 g/L
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not specified
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: not specified
- Preliminary purification step (if any): not specified
- Final dilution of a dissolved solid, stock liquid or gel: not specified
- Final preparation of a solid: not specified
FORM AS APPLIED IN THE TEST (if different from that of starting material): not specified - Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 2.0 g of the test substance was added to about 800 mL of demineralized water and stirred at room temperature until the test substance was completely dissolved. After that the pH-value was measured (measured value = 7.3). The stock solution was made up to 1L with demineralized water.
- Eluate: not applicable
- Differential loading: not applicable
- Controls: To prepare the blank control assays 150 mL of demineralized water and 8 mL synthetic medium were given to the blank control test vessels.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): "The stock solution appeared clear and no undissolved test substance was visible". - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
activated sludge from a municipal wastewater treatment plant
- Name and location of sewage treatment plant where inoculum was collected: Mannheim, Germany
- Method of cultivation: no data
- Preparation of inoculum for exposure: The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated overnight at room temperature.
- Pretreatment: The next day the sludge suspension was washed once with tap water and the suspension was adjusted to 7.5 g/L dry matter.
- Initial biomass concentration: "An aliquot of this suspension was added to the test vessels to obtain a sludge concentration of 1.5 g/L dry substance." - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- As recommended in applicable guideline
- Post exposure observation period:
- Not applicable
- Hardness:
- No data
- Test temperature:
- 20.5 - 20.8 °C
- pH:
- 7.1-7.2 [7.9-8.0 after incubation].
- Dissolved oxygen:
- Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement: > 6.5 mg/L - Salinity:
- Not applicable
- Conductivity:
- No data
- Nominal and measured concentrations:
- 62.5, 125, 250, 500 or 1000 mg/L (nominal). 62.4, 125, 250, 500 or 1000 mg/L (actual).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer-flasks
- Type (delete if not applicable): no data
- Material, size, headspace, fill volume: nominal volume 250 mL
- Aeration: "with pressure air" [not further specified]
- No. of vessels per concentration (replicates): 1
- No. of vessels per blank control (replicates): 2
- No. of vessels per vehicle control (replicates): not applicable
- No. of vessels per abiotic control (replicates): not applicable
- Sludge concentration (weight of dry solids per volume): 1.5 g/L
- Weight of dry solids per volume of reaction mixture per unit of time: no data
- Nutrients provided for bacteria: no data
- Nitrification inhibitor used: not applicable
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: [synthetic medium] 8 mL/test vessel of 100-fold concentrated OECD medium
- Particulate matter: no data
OTHER TEST CONDITIONS
- Adjustment of pH: not considered neccesary
- Photoperiod: not specified
- Light intensity: not specified
- Details on termination of incubation: "After 180 minutes incubation the mixtures in the test vessels were placed subsequently into a closed oxygen measuring cell".
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen respiration rates (oxygen consumption) measured for 8-10 minutes after incubation
TEST CONCENTRATIONS
- Spacing factor for test concentrations: "According to the test guidelines, at least 5 concentrations in a geometric series with a separation factor of ≤ 3.2 were used".
- Justification for using fewer concentrations than requested by guideline: not applicable
- Range finding study : not specified - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 510 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
no data
- Effect concentrations exceeding solubility of substance in test medium: not applicable
- Adsorption (e.g. of test material to the walls of the test container): no data
- Blank controls oxygen uptake rate: 1.5 mg/L in 6 minutes (15 mg/L/hr)
- Coefficient of variation of oxygen uptake rate in control replicates: not applicable (both blank controls had same uptake rate) - Results with reference substance (positive control):
- - Results with reference substance valid? Yes (within the range of 5-30 mg/L after 180 minutes)
- Relevant effect levels: EC50 7.2 mg/L - Reported statistics and error estimates:
- The oxygen consumption rates were determined after 180 minutes of exposure and used to calculate the inhibition effects at particular test concentrations compared to the mean of the 2 blank controls. These values were used for the determination of the ECx by the probit method based on Finney with the software TOXRAT. The values were given with an accuracy of 2 significant digits.
- Validity criteria fulfilled:
- yes
- Remarks:
- The deviation of the two blank controls is <15 % O2 consumption from the higher value and the EC50 of 3,5-dichlorophenol is in the range of 5-30 mg/L.
- Conclusions:
- In a guideline study, to GLP, sodium nitrite was studied for its ability to inhibit the respiration (oxygen consumption) of freshwater activated domestic sewage under static conditions. The 3-hr EC50 for sodium nitrite was determined to be 510 mg/L.
- Executive summary:
In an OECD Test Guideline 209 study, conducted according to GLP, the ability of sodium nitrite to inhibit the respiration (oxygen consumption) of freshwater activated domestic sewage was investigated under static conditions.
The test substance was added to culture medium at (nominal) concentrations of 0 (blank control), 62.5, 125, 250, 500 or 1000 mg/L and incubated for 3 hours. The reference substance (positive control; 2,5-dichlorophenol) was treated in a similar way. Following this, the respiration rate (oxygen consumption) of each treated and control inoculum was measured for 6 minutes. The oxygen consumption rates were used to calculate the inhibition effects at each test concentration (relative to the controls) and the degree of inhibition was subsequently evaluated by Probit analysis.
The blank controls and reference substance behaved as expected, confirming the validity of the study. The 3-hr EC50 for sodium nitrite was determined to be 510 mg/L.
Reference
The results with the reference substance indicate that the microorganisms are responding normally to toxicant stress.
Respective (rounded) EC10, EC20, EC50 and EC80 values of 210, 280, 510 and 940 mg/L were calculated for the test material.
Description of key information
No data on calcium nitrite were identified.
In a GLP study conducted according to OECD Test Guideline 209, sodium nitrite was studied for its ability to inhibit the respiration (oxygen consumption) of freshwater activated domestic sewage under static conditions. The 3-hr EC50 for sodium nitrite was determined to be 510 mg/L (Hammer, 2010).
Key value for chemical safety assessment
Additional information
No data on calcium nitrite were identified.
In an OECD Test Guideline 209 study, conducted according to GLP, the ability of sodium nitrite to inhibit the respiration (oxygen consumption) of freshwater activated domestic sewage was investigated under static conditions. The test substance was added to culture medium at (nominal) concentrations of 0 (blank control), 62.5, 125, 250, 500 or 1000 mg/L and incubated for 3 hours. The reference substance (positive control; 2,5-dichlorophenol) was treated in a similar way. Following this, the respiration rate (oxygen consumption) of each treated and control inoculum was measured for 6 minutes. The oxygen consumption rates were used to calculate the inhibition effects at each test concentration (relative to the controls) and the degree of inhibition was subsequently evaluated by Probit analysis. The blank controls and reference substance behaved as expected, confirming the validity of the study. The 3-hr EC50 for sodium nitrite was determined to be 510 mg/L (Hammer, 2010).
Sodium nitrite is closely related to calcium nitrite, and is considered a suitable surrogate for read-across for this endpoint. [See read-across justification report in IUCLID section 13 for details.]
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