Registration Dossier

Administrative data

Endpoint:
endocrine system modulation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Reliable study report (not GLP)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2003

Materials and methods

Principles of method if other than guideline:
11 kinds of UV stabilizers used in food contact plastics were tested for their estrogenic activity by the yeast two-hybrid assay.
GLP compliance:
no
Type of method:
in vitro
Endpoint addressed:
other: estrogenic activity

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
other: not applicable, in vitro assay
Strain:
other: not applicable

Administration / exposure

Route of administration:
other: not applicable
Vehicle:
other: DMSO
Details on exposure:
- The yeast cells were preincubated overnight at 30°C with vigorous shaking in a synthetic dropout (SD) medium free from tryptophan and leucine.
- test chemical solution was added and the mixture was incubated for 4 h at 30°C.
Duration of treatment / exposure:
4 h
Doses / concentrations
Remarks:
Doses / Concentrations:
1 mM - 0.1 µM
Basis:
nominal conc.

Examinations

Examinations:
The results were evaluated based on relative activity, expressed as 10% relative effective concentration (REC 10), which is the concentration of the
test chemical showing 10% of the agonist activity of 1 E-6 M 17ß-Estradiol, the highest activity level of 17ß-Estradiol.
Positive control:
17ß-Estradiol

Results and discussion

Details on results:
REC 10 < 1.0 E -4
None of the tested Benzotriazole compounds - among these the test substance - showed estrogenic activity.

Applicant's summary and conclusion