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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 18-20, 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 202 "Daphnia sp., Acute Immobilisation Test. and Reproduction Test".

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Principles of method if other than guideline:
None
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples were taken from all concentrations at the beginning of the study and after 72 hours. After preparation of the test media the analytical values were determined.

Test solutions

Vehicle:
no
Details on test solutions:
Reconstituted water according to ELENDT (1990):
Macro nutrients (mg/L):
CaCI2 x 7 H20 293.80
MgSO4 x 7 H20 123.30
NaHCO3 64.80
KCI 5.80
Na2SiO3 x 9H20 10.00
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18

Trace elements (mg/L):
B 0.5000
Fe 0.2000
Mn 0.1000
Li, Rb and Sr 0.0500
Mo 0.0250
Br 0.0125
Cu and Zn 0.0063
Co and I 0.0025
Se 0.0010
V 0.0003

Macro nutrients (mg/L):
Na2EDTA x 2H20 2.50

Vitamins (µg/L):
Thiamine 75.00
B12 1.00
Biotin 0.75


After preparation, the reconstituded water was aerated for 24 hours.

- Hardness: about 14° dH (about 250 mg/L CaCO3)
- pH: 7.9 ± 0.3, after an aeration for 24 hours.



References:

ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna Straus
Breeder: in house
Age: not older than 24 hours
This species is internationally accepted for this type of study.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
14 ° dH (about 250 mg/L CaCO3)
Test temperature:
21 – 22°C
pH:
7.7 ± 0.1
Dissolved oxygen:
7.8-8.2 mg /L
Nominal and measured concentrations:
100.0 mg/L nominal = 4.34 mg/L measured
Details on test conditions:
for details see executive summary
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
for details see executive summary
Results with reference substance (positive control):
for details see executive summary
Reported statistics and error estimates:
for details see executive summary

Any other information on results incl. tables

for details see executive summary

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Phenophthalein, solved in reconstituted water, was tested in an open static test system. An aqueous solution of 100 mg/L of Phenolphthalein revealed no aquatic toxicity in the test system.
The 48 hours EC50 to daphnids was > 4.34 mg/L (nominal > 100 mg/L) and, thus, could not be determined in this test.
Executive summary:
Purpose
The purpose of this assay was to identify the aquatic toxicity potential of the test item in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments

Study Design
For this purpose, juvenile daphnids were exposed to an aqueous test material solution over 48 hours, under defined conditions. The study comprised of four test vessels per concentration and control group containing five daphnids each, i.e. 20 daphnids per concentration (test medium group) and control group.
Daphnids were exposed to a nominal test material concentration of 100 mg/L equivalent to a measured concentration of 4.34 mg/L (limit test) in an open static system.


Results
The limit of quantification of the analytical method was 1.0 mg/L. Due to the low water solubility of the test material, the analytically determined test material concentrations were 4.3 % immediately after preparation of the medium and 48 hours thereafter. During the experimental phase of the study, the test material concentration could be maintained at 80 % of the initial concentration. The test was performed as a static test in open vessels. The EC50 values were calculated with the mean analytical concentrations due to the low solubility of the test material. Nominal, corresponding mean analytical concentrations and motility are given in the following table.

Nominal concentration Mean analytical concentration
during the study
Immobilized/exposed daphnids
[mg/L] [%] [mg/L] 24 hours 48 hours
0 - - 0/20 0/20
100 4.34 4.34 0/20 0/20

Daphnids exposed to an aqueous preparation of the nominal concentration of 100 mg/L were not affected. For the test material, the following nominal EC50 values for daphnids were determined: 24 h EC50 > 4.34 mg/L (nominal > 100 mg/L) 48 h EC50 > 4.34 mg/L (nominal > 100 mg/L)


Conclusion
Phenolphthalein, solved in reconstituted water, was tested in an open static test system. An aqueous solution of 100 mg/L of Phenolphthalein revealed no aquatic toxicity in the test system. The 48 hours EC50 to daphnids was > 4.34 mg/L (nominal > 100 mg/L) and, thus, could not be determined in this test.