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EC number: 211-334-3
CAS number: 638-38-0
The effects of Manganese (II) acetate on aquatic invertebrates was assessed in a weight-of-evidence approach in various species on several read-across substances:Espiritu EQ 1995: Acetic acid, Artemia nauplii, EC50 (24h) = 134 µl/L, EC50 (48h) = 132 µl/LJanssen CR 1993: Acetic acid, Daphnia magna, EC50 (24h) = 71 ± 3 µl/L, EC50 (48h) = 65 ± 9 µl/LSchweiger G 1956: Mn2+, corrected values for Manganese (II) acetate, 7 daysCarinogammarus Roeselii, LC0 = 45 mg/L, LC100 = 210 mg/LTubifex tubifex, LC0 = 600 mg/L, LC100 = 2100 mg/LChironomus thummy larvae, LC0 = 750 mg/L, LC100 = 3000 mg/LAnabolia nervosa larvae, LC0 = 3000 mg/L, LC 100 = 6000 mg/LBringmann G 1959: MnCl2, Daphnia magna, LC50 (48h) ≤ 50 mg/LKhangarot BS 1991: MnSO4 (values referring to element Mn), Tubifex tubifex, EC50 (24h) = 301.3 mg/L, EC50 (48h) = 208.6 mg/L, EC50 (96h) = 170.61 mg/LRathore RS 2002: MnSO4 (values referring to element Mn), Tubifex tubifex, EC50 (24h) = 422.36 mg/L, EC50 (48h) = 350.16 mg/L, EC50 (72h) = 275.7 mg/L, EC50 (96h) = 275.7 mg/LBengtsson B-E 1978: MnCl2, corrected value for Manganese (II) acetate, Nitocra spinipes, LC50 (96h) = 220.5 mg/LThe most relevant value for further risk assessment was determined to be LC0 (7 d) of 45 mg/L in Carinogammarus Roeselii.
(II) acetate consists of two ions, namely the Manganese (II) cation and
acetate anion. The existing data suggest to follow the approach of
assessing the two ions individually and perform the subsequent risk
assessment, i.e. deriving PNECs, based on the more distressing ion.
the acetate anion, an EC50 value of 132 µl/L (approx. 132 mg/L) after 48
h was found in Artemia nauplii (Espiritu EQ, Environmental Toxicology
and Water Quality: An International Journal, Vol. 10 (1995) 25-34 0 1995
by John Wiley & Sons, Inc.), and an EC50 (48 h) of 65 ± 9 µl/L,
corresponding to approx. 65 mg/L, was found in Daphnids. Taking into
account the sensitivity of daphnids towards acidic conditions and the
fact, that the pH was not adjusted in both experiments, the EC50 value
would be much higher if pH was adjusted, preferably above 100 mg/L as
already shown in Artemia nauplii. Additionally, it can be concluded from
the magnitude of the observed EC50s, the NOEC in a chronic study will be
above 1 mg/L. Acetic acid is part of many metabolic pathways, has an
ionic structure and low molecular weight, so it can be concluded, that
it has a low BCF and no toxic metabolites will be formed. In Summary,
the observed EC50 values obtained from acetic acid and subsequent
conclusions do not trigger classification. Consequently, Acetic acid can
considered to be non-harmful for aquatic life, especially as it can be
assumed that the toxic effects result mainly from the changes in the
test water pH which was not adjusted in these experiments. So it can be
furthermore concluded that the acetate anion contained in manganese (II)
acetate poses no harm to aquatic life and it will be sufficient that all
further risk assessment is based on the manganese cation only.
assessing the toxicity of the manganese (II) cation, most studies on the
read across substances exhibited EC50, LC50, LC0 and LC100 values above
100 mg/L on several timepoints up to 7 d:
Mn2+, corrected values for Manganese (II) acetate, 7 days:
Carinogammarus Roeselii, LC0 = 45 mg/L, LC100 = 210 mg/L; Tubifex
tubifex, LC0 = 600 mg/L, LC100 = 2100 mg/L; Chironomus thummy larvae,
LC0 = 750 mg/L, LC100 = 3000 mg/L; Anabolia nervosa larvae, LC0 = 3000
mg/L, LC 100 = 6000 mg/L (Schweiger G, “Die toxikologische Einwirkung
von Schwermetallsalzen auf Fische und
Fischnährtiere”,Inaugural-Dissertation zur Erlangung der Doktorwürde der
Hohen Naturwissenschaftlichen Fakultät der
Ludwig-Maximilians-Universität zu München, Februar 1956)
MnCl2, Daphnia magna, LC50 (48h)≤50
mg/L (Bringmann G, Gesundheits-Ingenieur, Heft 4 (80. Jahrgang 1959),
MnSO4 (values referring to element Mn), Tubifex tubifex, EC50 (24h) =
301.3 mg/L, EC50 (48h) = 208.6 mg/L, EC50 (96h) = 170.61 mg/L (Khangarot
BS, Bull. Environ. Contam. Toxicol. (1991) 46:906-912, Springer-Vedag
New York Inc.)
MnSO4 (values referring to element Mn), Tubifex tubifex, EC50 (24h) =
422.36 mg/L, EC50 (48h) = 350.16 mg/L, EC50 (72h) = 275.7 mg/L, EC50
(96h) = 275.7 mg/L (Rathore RS, Ecotoxicologyand Environmental Safety
53, 27}36 (2002) Environmental Research, Section B
MnCl2, corrected value for Manganese (II) acetate, Nitocra spinipes,
LC50 (96h) = 220.5 mg/L (Bengtsson BE, Marine Pollution Bulletin, Vol.
9. pp. 238-241, Pergamon Press Ltd. 1978.)
high EC50, LC50, LC0 and LC100 values determined in aquatic invertebrate
studies covering this endpoint do not reasonable indicate the necessity
to perform long-term toxicity test because the NOEC which would be
determined in these test is expected to be very high, too, i.e. much
higher than the boundary value of 1 mg/L, which is especially supported
by the LC0 values determined by Schweiger, 1956, after 7 days.
Additionally, the manganese (II) cation as an inorganic ion is neither
expected to have a BCF≥500
nor a logKow≥4.
Taking into account the classification criteria as set out in regulation
1272/2008/EC, Mn2+ does not trigger classification, and consequently,
Manganese (II) acetate does not need to be classified as well as no
further long-term testing is indicated.
the key value (freshwater invertabrates) for further risk assessment the
LC0 (7d) of 45 mg/L in Carinogammarus Roeselii was chosen. Since the
determined EC50, LC50, LC0 and LC100 values do not indicate further
long-term testing, the possibly best surrogate for a chronic NOAEL
should be chosen. 7 days is the longest test duration available and the
value LC0 indicates that no animal was affected, which corresponds very
well to a No Observed Adverse Effects Level. Furthermore, Carinogammarus
Roeselii was determined to be the most sensitive species. Consequently,
when performing the further assessment of Manganese (II) acetate
toxicity and deriving PNECS, it must be taken into account that 45 mg/L
is not the usually used EC50 (48 h) value but the LC0 (7 d) value and
subsequently lower assessment factors should be applied.
EC50 for marine water invertebrates of 132 mg/L was chosen on the basis
of the effects of Acetic acid on Artemia nauplii, although it
is most likely to be caused by changes in the pH, because it is the only
value available for marine water invertebrates.
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