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EC number: 931-745-8 | CAS number: 1335203-21-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-05-6 to 2010-07-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure:
Fresh samples of activated sludge are withdrawn on June 22th, 2010 from the sewage treatment plant Ruhrverband Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany, which is mainly fed with municipal wastewater. On return to the laboratory the sludge was washed three times with isotonic solution which is in accordance with the guideline.
- Pretreatment: Subsequently, mixed liquor suspended solids level of the sludge was
adjusted on about 4.0 g/L. The solution was kept aerated at 20 ± 2 °C until test start.
- Initial biomass concentration: The concentration used in the test was 1.61 g dry mass/litre. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18-22 °C
- pH:
- 7.2
- Nominal and measured concentrations:
- Nominal concentrations of test substance: 95.3, 171.5, 308.6, 555.6, and 1,000 mg/l.
Nominal concentrations of the reference substance: 4, 12, and 36 mg/l. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Conical flask
- Material, size, headspace, fill volume: 1 liter volume glass flasks. Fill volume of 500 ml
- Aeration: Contents were aerated during incubation (0.75 l/min)
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):2 and 1 control withouth inoculum
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water free from inhibitory conentrations of toxic substances was used.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Respiration rates were measured and recorded over a period of 10 minutes.
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 95.3, 171.5, 308.6, 555.6, and 1,000 mg/l.
- Results used to determine the conditions for the definitive study: Results from range finding study indicated that the EC50 is somewhere in the range of 500-600 mg/l. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 564 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL = 364-887 mg/l
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 308.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 555.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none reported
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: The EC50 of 3,5-dichlorophenol for respiration inhibition was found to be within the range of 5.0 and 30.0 mg/l. - Reported statistics and error estimates:
- Numerical values in this report are frequently rounded to a smaller degree of precision (number of digits) than were used in the actual calculation. Minor differences in results obtained from calculations with such rounded values in comparison to those obtained with higher precision values are possible. They are, however, well within the limits of the experimental accuracy and thus of no practical concern.
- Calculation of the respiration rates and the percent inhibition of these rates was performed according to the guideline and listed in a table.
- The test results were statistically analyzed using the Probit-analysis of the computer program (ToxRat) assuming log-normal distribution of the values. - Validity criteria fulfilled:
- yes
- Remarks:
- The validity crieria of the test were fulfilled. The pH of the test medium remained in the range of 6.0 -8.0, the incubation temperature remained in the range of 18 -22 °C, the EC50 of the reference substance (3,5 -dichlorphenol) was in the the accepted r
- Conclusions:
- In a test according to EU Method C.11, the 3h EC50 of the test substance is 564 mg/l with a 95% confidence limit of 364 -887 mg/l. The NOEC was found to be 308.6 mg/l and the LOEC 555.6 mg/l.
- Executive summary:
In a test according to EU Method C.11, the 3h EC50 of the test substance is 564 mg/l with a 95% confidence limit of 364 -887 mg/l. The NOEC was found to be 308.6 mg/l and the LOEC 555.6 mg/l.
Reference
Table 1: Inhibition (%) of the respiration rate by the test item
Test Concentration |
||||
95.3 mg/l |
171.5 mg/l |
308.6 mg/l |
555.6 mg/l |
1000 mg/l |
4.4% |
12.6% |
8.1% |
47.2% |
94.2% |
Description of key information
The 3h EC50 of the test substance is 564 mg/l. The NOEC was found to be 308.6 mg/l and the LOEC 555.6 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 564 mg/L
Additional information
In a test according to EU Method C.11, the 3h EC50 of the test substance is 564 mg/l with a 95% confidence limit of 364 -887 mg/l. The NOEC was found to be 308.6 mg/l and the LOEC 555.6 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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