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EC number: 951-779-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2020-10-14 to 2020-11-06
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Analysis were performed by Total organic Carbon measurements at initial time (T=0h) and at final time (T=48h). At T0h, All the solutions were analysed taken directly from the starting solutions without daphnids. At T48h, all the solutions were sampled from test tubes containing daphnids.
Observations of the daphnid’s immobility and behaviour were done after 24 and 48 hours in Control and exposed groups.
Any sign of stress observed on the daphnids after 24 and 48 hours of exposure to the test item were reported.
pH and dissolved oxygen were measured at the start and at the end of the test (including Controls). - Details on test solutions:
- Seven test solutions of required concentrations were prepared by direct addition (by weighing) from the test item with the daphnids medium.
The prepared samples were stirred during 24 hours at 20 ± 2°C, darkness. After this step, the samples were filtrated on cellulose acetate filter (porosity: 0.45 µm – batch A26689264).
The test solutions were then filtered through a membrane filter: solutions obtained are named WAF (Water Accommodated Fraction).
Each volume of the daphnids test tubes was approximately 10 mL. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna Straus, clone number 5, from at least the third generation (i.e. the third brood) obtained with a non-cyclic parthenogenesis reproduction.
The daphnids breeding was carried out in closed bottles placed in a climatic chamber free from any toxic vapour and in medium M4.
Daphnids used in this experimentation were previously filtered in order to obtain animals from a size between 560 µm and 800 µm (corresponding to daphnids less than 24 hours old). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- No
- Test temperature:
- 20.0 - 20.2°C
- pH:
- The pH of the solutions did remain in the range 6 – 9, no pH adjustment was necessary.
- Nominal and measured concentrations:
- Loading rates: 0, 5, 10, 18, 32, 60, 105, 190 mg test item /L
- Details on test conditions:
- The protocol consisted of exposing young daphnids at seven test item concentrations over a period of 48 hours in static conditions. Immobilisation of daphnids was recorded at 24 hours and 48 hours and compared with Control values.
The test was carried out under static conditions, which means that the test solution were not renewed at 24h.
The test contained a minimum of 32 glass test tubes as follow:
• Control: 4 replicates
• Loading rate 5 mg/L: 4 replicates
• Loading rate 10 mg/L: 4 replicates
• Loading rate 18 mg/L: 4 replicates
• Loading rate 32 mg/L: 4 replicates
• Loading rate 60 mg/L: 4 replicates
• Loading rate 105 mg/L: 4 replicates
• Loading rate 190 mg/L: 4 replicates
The test was performed in the dark between 18 and 22°C but constant within ±1°C. Daphnids were not fed during the test period.
Observations of the daphnid’s immobility and behaviour were done after 24 and 48 hours in Control and exposed groups.
Any sign of stress observed on the daphnids after 24 and 48 hours of exposure to the test item were reported.
pH and dissolved oxygen were measured at the start and at the end of the test (including Controls). - Reference substance (positive control):
- yes
- Remarks:
- Potassium Dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 135 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Loading rates
- Basis for effect:
- mobility
- Remarks on result:
- other: Cl : 105 -198
- Results with reference substance (positive control):
- The last toxic response obtained on the reference item K2Cr2O7 (November 12, 2020) indicates an EC50-24h of 1.43 mg/L (1.30 – 1.57), indicating that the daphnids sensitivity is correct (within the accepted interval of 0.6 – 2.1 mg/L fixed by the International Standard ISO 6341 December 2012 as mentioned in the OECD 202 Guideline) and in accordance with historical data obtained by the laboratory.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results obtained for the test item “TOBACCO ABSOLUTE” on the toxicological endpoint “immobilisation” of daphnids give an EL50-48h = 135 mg/L [105 - 198], based on test item loading rate.
- Executive summary:
The aim of this study was to determine the acute toxicity of the test item “TOBACCO ABSOLUTE” on the daphnids Daphnia magna, according to the OECD guideline 202, in static-conditions.
This study was carried out in accordance with the study plan no.20/1044F/d dated November 19, 2020.
There was no deviation and no amendment during this study.
Following the results of preliminary tests (non GLP), a final test, at seven nominal test item concentrations was performed, in order to evaluate the concentration which immobilise 50% of the daphnids exposed to the test item after 24 and 48 hours, reported as EL50-24h and EL50-48h, relative to tested loading rates.
The immobility of the daphnids was determined in a closed static 48-hours test by visual observation after 24 and 48 hours.
The concentration of “TOBACCO ABSOLUTE” in the Control and the solutions was checked by Carbon analyser.
Observation time
24h
48h
EL10
Between 105 and 190 mg/L
52 mg/L [34 - 68]
EL50
≥190 mg/L
135 mg/L [105 - 198]
Reference
pH and dissolved oxygen values during the final test
Nominal test item loading rate (mg/L) | pH | |
T0 | T 48h | |
WAF Control | 7.8 | 7.6 |
WAF 5 | 7.9 | 7.6 |
WAF 10 | 7.9 | 7.5 |
WAF 18 | 7.8 | 7.5 |
WAF 32 | 7.8 | 7.5 |
WAF 60 | 7.8 | 7.5 |
WAF 105 | 7.8 | 7.4 |
WAF 190 | 7.8 | 7.2 |
Nominal test item loading rate (mg/L) | O2 (mg/L) | |
T0 (Air: 9.5 mg/L) | T48 h (Air: 9.3 mg/L) | |
WAF Control | 9.3 | 9.1 |
WAF 5 | 9.2 | 9.0 |
WAF 10 | 9.1 | 8.8 |
WAF 18 | 9.2 | 8.8 |
WAF 32 | 9.1 | 8.7 |
WAF 60 | 9.0 | 8.7 |
WAF 105 | 8.9 | 8.6 |
WAF 190 | 8.9 | 8.7 |
Description of key information
The aim of this study was to determine the acute toxicity of the test item “TOBACCO ABSOLUTE” on the daphnids Daphnia magna, according to the OECD guideline 202, in static-conditions.
This study was carried out in accordance with the study plan no.20/1044F/d dated November 19, 2020.
There was no deviation and no amendment during this study.
Following the results of preliminary tests (non GLP), a final test, at seven nominal test item concentrations was performed, in order to evaluate the concentration which immobilise 50% of the daphnids exposed to the test item after 24 and 48 hours, reported as EL50-24h and EL50-48h, relative to tested loading rates.
The immobility of the daphnids was determined in a closed static 48-hours test by visual observation after 24 and 48 hours.
The concentration of “TOBACCO ABSOLUTE” in the Control and the solutions was checked by Carbon analyser.
Observation time |
24h
| 48h |
EL10 |
Between 105 and 190 mg/L
| 52 mg/L [34 - 68] |
EL50 | ≥190 mg/L | 135 mg/L [105 - 198] |
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 135 mg/L
Additional information
The influence of the test substance on immobilisation of Daphnia magna was investigated according to OECD Guideline 202, with GLP statement. Daphnids were exposed to the test item under static conditions, for 48 h.
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