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EC number: 950-112-7 | CAS number: 347377-00-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03-10-2019 - 01-11-2019 (experimental phase)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- see "Principles of method if other than guideline"
- Principles of method if other than guideline:
- A minor deviation from the guidelines of the Closed Bottle test (OECD TG 301D) was introduced; ammonium chloride was omitted from the medium to prevent oxygen consumption due to nitrification (omission does not result in nitrogen limitation as shown by the biodegradation of the reference compound).
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,4-Hexadienoic acid, 1,1’-[2-ethyl-2-[[(1-oxo-2,4-hexadien-1-yl)oxy]methyl]-1,3-propanediyl] ester
- EC Number:
- 950-112-7
- Cas Number:
- 347377-00-8
- Molecular formula:
- C24H32O6
- IUPAC Name:
- 2,4-Hexadienoic acid, 1,1’-[2-ethyl-2-[[(1-oxo-2,4-hexadien-1-yl)oxy]methyl]-1,3-propanediyl] ester
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: River water without particles was used as inoculum
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): River water was sampled from the Rhine near Heveadorp, The Netherlands (26-09-2019). The nearest plant (Arnhem-Zuid) treating domestic wastewater biologically was 3 km upstream.
- Preparation of inoculum for exposure: The river water used in the Closed Bottle test was spiked per liter of water with 8.51 mg KH2PO4, 21.75 mg K2HPO4, 33.42 mg Na2HPO4.2H2O, 22.50 mg MgSO4·7H2O, 27.51 mg CaCl2, 0.25 mg FeCl3·6H2O.Ammonium chloride was not added to the river water to prevent nitrification. Sodium acetate was added to the bottles using a stock solution of 1.0 g/L. The test item, a poorly water-soluble substance was administered using a stock solution of 1.0 g/L in dichloromethane.
- Pretreatment: The river water was aerated for 7 days before use to reduce the endogenous respiration (van Ginkel and Stroo, 1992). River water without particles was used as inoculum. The particles were removed by sedimentation after 1 day while moderately aerating. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2 mg/L
- Based on:
- not specified
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- see "Any other information on materials and methods incl. tables"
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 62
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The biodegradation percentage of the reference compound, sodium acetate, at day 14 was 83
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biodegradation in water (screening) was determined in a well-documented guideline study compliant to GLP. Therefore, it can be considered as reliable without restrictions (Reliability 1). According to the results of this study, the test item did not cause a reduction in the endogenous respiration at day 7. The test substance is therefore considered to be non-inhibitory to the inoculum. The test item was biodegraded by 62% at day 28 in the OECD 301D Closed Bottle test. The time window criterion should not be applied as a pass/fail criterion because test sample is a poorly water soluble substance. Test sample is therefore classified as readily biodegradable based on the >60% biodegradation reached at day 28. The test is valid as shown by an endogenous respiration of 0.75 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded by 83% of its theoretical oxygen demand after 14 days. Finally, the most important criterion was met by oxygen concentrations >0.5 mg/L in all bottles during the test period.
- Executive summary:
To assess the biotic degradation of the test sample, a ready biodegradability test was performed which allows the biodegradability to be measured in an aerobic aqueous medium. The ready biodegradability was determined in the Closed Bottle test performed according to slightly modified OECD 310D and in compliance with the OECD principles of GLP.
The test item (2 mg/L) was exposed to river water which was spiked with nutrients, dosed in closed bottles and incubated in the dark at 22.4-22.9°C for 28 days. The degradation of the test item was assessed by the measurement of oxygen consumption. According to the results of this study, the test item did not cause a reduction in the endogenous respiration at day 7. The test substance is therefore considered to be non-inhibitory to the inoculum.
The test item was biodegraded by 62% at day 28 in the OECD 301D Closed Bottle test. The time window criterion should not be applied as a pass/fail criterion because test sample is a poorly water soluble substance. Test sample is therefore classified as readily biodegradable based on the >60% biodegradation reached at day 28.
The test is valid as shown by an endogenous respiration of 0.75 mg/L and by the total mineralization of the reference compound, sodium acetate. Sodium acetate was degraded by 83% of its theoretical oxygen demand after 14 days. Finally, the most important criterion was met by oxygen concentrations >0.5 mg/L in all bottles during the test period.
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