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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- From August 02, 1990 to September 03, 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Refer to the Quaternary ammonium salts (QAS) category or section 13 of IUCLID for details on the category justification. The study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Instead of using 3,5-dichlorophenol as reference (acc. to OECD 209) potassium dichromate was used in a concentration to inhibit respiration of 50%.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable as nominal test concentrations are evaluated
- Vehicle:
- no
- Details on test solutions:
- No further details available
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: activated sludge from municipal waste water treatment plant Hildesheim
- Method of cultivation:
- Preparation of inoculum for exposure: washed twice with dechlorinated, autoclaved tap water
- Pretreatment: none
- Initial biomass concentration: 1.251 g/L dry weight - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Not applicable
- Hardness:
- Not specified
- Test temperature:
- 22°C
- pH:
- Not specified
- Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 10 - 18 - 32 - 58 - 100 - 180 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:500 mL Erlenmeyer flasks
- Type (delete if not applicable): not specified
- Material, size, headspace, fill volume: 500mL
- Aeration: yes, permanently
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate):not specified
- No. of organisms per vessel:not specified
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water acc. to OECD 209
OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
- Light intensity: not specified - Reference substance (positive control):
- yes
- Remarks:
- kaliumdichromat
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 9.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- Respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- Respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 43 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- Respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- Respiration rate
- Details on results:
- No further details available
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 32 - 58 - 100 mg/ L - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 3 h EC 10 and EC50 (in activated sludge) of the test substance was determined to be 9.5 and 43 mg/L, respectively.
- Executive summary:
A study was conducted to determine the toxicity of the read across substance, C20-22 TMAC (active content not specified), to microorganisms according to OECD Guideline 209 (Activated Sludge Respiration Inhibition Test). Approximately 1.251 g/L dry weight of activated sludge (domestic) was exposed to the test substance at nominal concentrations of 10, 18, 32, 58, 100 and 180 mg/L for 3 h. A positive control group (Potassium dichromate) was included in the experiment and gave values within the expected range. Under the study conditions, the 3 h EC10 and EC50 (in activated sludge) of the test substance was determined to be 9.5 and 43 mg/L (nominal), respectively (Hildesheim, 1990). Based on the results of the read across study, similar EC50 values can be expected for the test substance, C18-22 TMAC.
Reference
No further details available
Description of key information
Based on the results of the read across study, the 3 h EC10 and EC50 (in activated sludge) of the test substance, C18-22 TMAC, is considered to be 9.5 and 43 mg/L, respectively (nominal).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 43 mg/L
- EC10 or NOEC for microorganisms:
- 9.5 mg/L
Additional information
A study was conducted to determine the toxicity of the read across substance, C20-22 TMAC (active content not specified), to microorganisms according to OECD Guideline 209 (Activated Sludge Respiration Inhibition Test). Approximately 1.251 g/L dry weight of activated sludge (domestic) was exposed to the test substance at nominal concentrations of 10, 18, 32, 58, 100 and 180 mg/L for 3 h. A positive control group (Potassium dichromate) was included in the experiment and gave values within the expected range. Under the study conditions, the 3 h EC10 and EC50 (in activated sludge) of the test substance was determined to be 9.5 and 43 mg/L (nominal), respectively (Hildesheim, 1990). Based on the results of the read across study, similar EC50 values can be expected for the test substance, C18-22 TMAC, for toxicity to microorganisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.