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EC number: 700-330-1 | CAS number: 433733-92-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 July - 03 July 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations
- Sampling method:
Frequency at t=0 h and t=48 h
Volume 3 ml from the approximate centre of the test vessels
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
- Sample storage conditions before analysis: in a freezer until analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Preparation of test solutions started with the highest test solution of 100 mg/l. No special treatment was necessary to completely dissolve the test substance in the test medium. The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. The final test solutions were all clear and colourless. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Source: In-house laboratory culture with a known history.
- Age at study initiation (mean and range, SD): For the test selection of young daphnids with an age of < 24 hours, from parental daphnids of more than two weeks old.
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel.
- Feeding during test: noo feeding - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No post exposure observations.
- Hardness:
- 180 mg/l
- Test temperature:
- 19.5 - 20.0°C
- pH:
- 6.0-8.5
- Dissolved oxygen:
- >=3 mg/l at the end of the test
- Nominal and measured concentrations:
- See the table "Concentrations of the test substance in test medium" below
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: 100 ml, all-glass
- Aeration: No aeration of the test solutions.
- No. of organisms per vessel: 5 per vessel containing 80 ml of test solution
- No. of vessels per concentration (replicates): 4 for the highest concentration; 2 each for the lower concentration
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium
- Culture medium different from test medium: yes, M7 medium
OTHER TEST CONDITIONS
- Adjustment of pH: 7.7 ± 0.3.
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility (including mortality): At 24 hours and at 48 hours.
pH and dissolved oxygen: At the beginning and at the end of the test, for all concentrations and the control.
Temperature of medium: Continuously in a temperature control vessel, beginning at the start of the test.
TEST CONCENTRATIONS
- Test concentrations: 0.1, 1.0, 10 and 100 mg/l.
- Controls: Test medium without test substance or other additives. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: 5% at 0.32 mg/l after 48h, 15% at 0.56 mg/l after 48h, 100% at 1 mg/l after 48h
- EC50/LC50:
The 24h-EC50 was 0.77 mg/l with a 95% confidence interval between 0.68 and 0.91 mg/l.
The 48h-EC50 was 0.62 mg/l with a 95% confidence interval between 0.53 and 0.77 mg/l. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study the test substance did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50 exceeded 100 mg/l, the regulatory limit concentration.
Reference
Concentrations of the test substance in test medium
Time of sampling |
Concentration |
|||
Nominal [mg/l] |
Analysed |
Relative to nominal |
Relative to initial |
|
0 |
0 |
* |
n.a. |
|
100 |
102 |
102 |
||
48 |
0 |
* |
n.a. |
n.a. |
100 |
102 |
102 |
100 |
* A small response at the retention time of the test substance was observed. It was not considered to derive from the test samples since it was also observed in the analytical blanks.
- Recovery: The mean recovery of the procedural recovery samples was 99%. Because the criterion that mean recoveries should be between 70% and 110% was met, the results for the test samples were accepted.
Description of key information
Under the conditions of the present study the test substance did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC).
The 48h-EC50 exceeded 100 mg/l, the regulatory limit concentration.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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