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EC number: 925-425-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 16 September 1999 - 19 October 1999
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- VETYNAL
Lot No. : 9000349063
Purity : GLC profile matches standard - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aïre) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 32 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Water
The water used during this study is deionised water containing less than 10 mg/l dissolved organic carbon.
Preparation of the flasks
Test substance samples (30 mg, corresponding to 30 mg/l in a 1000-ml flask) are weighed in small aluminium boats and added directly to the test flasks of the BI-1000, whereas the reference substance sample (sodium benzoate) is added as 1.0 ml of a 100 mg/ml solution in mineral medium.
All flasks are filled with 1000 ml of mineral medium. Samples of test or reference substances are added. Then, a volume of suspended sludge corresponding to 100 mg dry weight (as determined from 3.7, generally 15 to 40 ml) is added. Except when the test substance has an acid or alkaline character, the pH of each flask is not measured but assumed to be the same as the mineral medium, in order not to remove any floating undissolved test substance from the test medium by dipping a glass electrode in it. Neutral test substances, even sodium benzoate, were shown not to affect the pH of the medium by more than 0.1 pH unit. About 5 g of soda lime is placed in an attachment of the stopper, the flasks are closed and placed in the water bath of the BI-1000. After temperature and pressure equilibration, the measuring cells are started (time zero of the experiment).
Performance of the test
The oxygen consumption of each flask is automatically recorded by the BI-1000 during the whole test duration. Correct temperature and stirring are regurlarly checked visually.
At the end of the test period, the pH of each flask is measured. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Purity : Minimum 99.0%
- Parameter:
- % degradation (O2 consumption)
- Value:
- 37
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 42
- Sampling time:
- 32 d
- Results with reference substance:
- Degradation of sodium benzoate is 77 % after 7 days and 88 % after 14 days, widely in excess of the validity criterium: the activity of the inoculum is thus verified and the test is considered valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- VETYNAL undergoes 37 % biodegradation after 28 days (and 42 % after 32 days) in the test conditions.
Thus, VETYNAL should be regarded as not inherently but partially biodegradable according to this test. - Executive summary:
VETYNAL undergoes 37 % biodegradation after 28 days (and 42 % after 32 days) in the test conditions.
Thus, VETYNAL should be regarded as not inherently but partially biodegradable according to this test,
with 37% of the starting Parent material completely mineralised in to CO2 and H2O by Day 28, increasing to 42% by Day 32.
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- February 04 - April 11, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name (as stated in the report): VETYNAL Extra
Batch: VE00375495
Expiration date: June 07, 2017
Purity: Conform - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 67 d
- Initial conc.:
- 30 mg/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Water
The water used during this study is ultrapure water, containing less than 5 ppb total organic carbon, produced by using a Millipore Direct-Q 3 UV purification system.
Preparation of the flasks
Test substance samples (13.47 mg, corresponding to 30.0 mg/l in 449 ml of test volume) are weighed in small aluminium boats and added directly to the test flasks of the Oxitop that were previously charged with 432 ml of mineral medium. Then, 17.1 ml of suspended sludge, diluted to a concentration of 2.62 g/l dry matter, corresponding to 44.8 mg dry weight is added. Flasks containing sludge only (100 mg/l) are charged with 432 ml of mineral medium. Then, 17.1 ml of suspended sludge, diluted to a concentration of 2.62 g/l dry matter, are added.
Reference substance samples (~43.70 mg, corresponding to 100 mg/l in 437 ml of test volume) are weighed in small aluminium boats and added directly to the test flasks of the Oxitop that were previously charged with 432 ml of mineral medium. Then, 5.0 ml of suspended sludge, diluted to a concentration of 2.62 g/l dry matter, corresponding to 13.1 mg dry weight is added. Flasks containing sludge only (30 mg/l) are charged with 432 ml of mineral medium. Then, 5.0 ml of suspended sludge, diluted to a concentration of 2.62 g/l dry matter, are added.
Except when the test substance has an acid or alkaline character, the pH of each flask is not measured but assumed to be the same as the mineral medium, in order not to remove any floating undissolved test substance from the test medium by dipping a glass electrode in it. Neutral test substances, even sodium benzoate, were shown not to affect the pH of the medium by more than 0.1 pH unit. Two sodium hydroxide pellets are placed in the quivers on top of the bottle, and the flasks are closed tightly with the measuring heads. The flasks are allowed to equilibrate to the test temperature. The measurement is started by programming the measuring unit of the Oxitop test flasks, and the test flasks are placed in the temperature controlled cupboard of the Oxitop system. After temperature equilibration, the controller of the instrument starts the data acquisition (time zero of the experiment).
Performance of the test
Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked.
At the end of the test period (normally 28 days), the pH of each flask is measured again. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- purity: min. 99.0 %
- Parameter:
- % degradation (O2 consumption)
- Value:
- 39
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 56
- Sampling time:
- 67 d
- Results with reference substance:
- Degradation of sodium benzoate exceeded 40 % after 7 days and 65 % after 14 days: the activity of the inoculum was thus verified (validity criterion).
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: inherently primarily biodegradable
- Conclusions:
- In the test conditions Vetynal Extra undergoes 39% biodegradation after 28 days (56% biodegradation after 67 days).
Vetynal Extra did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Vetynal Extra cannot be regarded as inherently and ultimately biodegradable according to this test. However, Vetynal Extra should be regarded as inherently primarily biodegradable according to this test. - Executive summary:
The Inherent Biodegradability of Vetynal Extra was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 302C.
In the test conditions Vetynal Extra undergoes 39% biodegradation after 28 days (56% biodegradation after 67 days).
Vetynal Extra did not significantly inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Vetynal Extra cannot be regarded as inherently and ultimately biodegradable according to this test. However, Vetynal Extra should be regarded as inherently primarily biodegradable according to this test with 39% of the starting Parent material completely mineralised in to CO2 and H2O by Day 28, injcreasing to 56% by Day 67.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 July 2015 - 19 October 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ECHA Guidance for the implementation of REACH, Guidance on information requirements and chemical safety assessment, Chapter R.7b: Endpoint specific guidance, November 2014 (version 2.0)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name (as stated in the report): VETYNAL Extra
Batch: VE00375495
Expiration date: June 07, 2017
Purity: Conform - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 62 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Preparation of the flasks
Test substance samples (13.10 mg, corresponding to 30.0 mg/l in 437 ml of test medium) are weighed in small aluminium boats and added directly to the test flasks of the Oxitop. For reference substance samples 43.70 mg (corresponding to 100.0 mg/l in 437 ml of test medium) are weighed in small aluminium boats and added directly to the test flasks of the Oxitop.
Flasks are filled with 432 ml of mineral medium. Samples of test or reference substance are added. Then 5.00 ml of suspended sludge diluted to a concentration of 2.62 g/l dry matter is added. Except when the test substance has an acid or alkaline character, the pH of each flask is not measured but assumed to be the same as the mineral medium, in order not to remove any floating undissolved test substance from the test medium by dipping a glass electrode in it. Neutral test substances, even sodium benzoate, were shown not to affect the pH of the medium by more than 0.1 pH unit. Two sodium hydroxide pellets are placed in the quivers on top of the bottle, and the flasks are closed tightly with the measuring heads. The flasks are allowed to equilibrate to the test temperature. The measurement is started by programming the measuring unit of the Oxitop test flasks, and the test flasks are placed in the temperature controlled cupboard of the Oxitop system. After temperature equilibration, the controller of the instrument starts the data acquisition (time zero of the experiment).
Performance of the test
Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked.
At the end of the test period, the pH of each flask is measured again. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 99% purity, concentration 100 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 12
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 41
- Sampling time:
- 62 d
- Results with reference substance:
- Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Vetynal Extra undergoes 12% biodegradation after 28 days (41% after 62 days) in the test conditions.
Vetynal Extra did inhibit the intrinsic respiration of the inoculum at the test concentration at the beginning of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.
Vetynal Extra should be regarded as not readily biodegradable according to this test. - Executive summary:
The Ready Biodegradability of Vetynal Extra was determined by the Manometric Respirometry Test.
Vetynal Extra undergoes 12% biodegradation after 28 days (41% after 62 days) in the test conditions.
Vetynal Extra did inhibit the intrinsic respiration of the inoculum at the test concentration at the beginning of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.
Vetynal Extra should be regarded as not readily biodegradable according to this test. Significant mineralisation was, nonetheless, observed during the study suggesting a high rate of metabolism of the parent.
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
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