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Diss Factsheets
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EC number: 605-460-1 | CAS number: 167004-78-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From Dec 1992 to Dec 11 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- yes
- Remarks:
- The Report was drawn in a simplified form. No inspection by the Quality Assurance Unit took place. There is thus no Quality Assurance Statement (test procedure according to GLP) included in this report.
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of stock solution:
1.25 g/L test material stirred for 18 h at 23-24 °C. The pH after stirring (3.8) was corrected to 7.1 with NaOH, 1 mol/l; HCL, 0.1 mol/l
The test material was clearly dissolved. The batch was used as stock solution. - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- The strain Pseudomonas putida Migula, DSM 50026 was used as inoculum. The pre-culture and inoculation of the test batch were carried out as stated in the cited DIN guideline (Section 11.2). Prior to the test, the bacterial culture was not acclimatised to the test substance or reference substance.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 16 h
- Remarks on exposure duration:
- ±1 hour
- Nominal and measured concentrations:
- test concentrations ranged from 0.1 mg/L to 250 mg/L
- Details on test conditions:
- The composition of the reaction medium corresponded to the data given in the cited DIN guideline.
Incubation took place at 21±1°C in Erlenmeyer flasks over a period of 16±1 hours. For aeration, the solutions were kept in suspension on a rotary shaker with a circular movement, 5 cm in diameter, and at approx. 220 revolutions per minute. The composition of the reaction medium corresponded to the data given in the cited DIN guideline.
The sensitivity of the bacterial culture was checked with a reference batch. 2,4-dichlorophenol was used as reference batch in the concentrations of
30, 70 and 100 mg/l. - Reference substance (positive control):
- yes
- Remarks:
- 2,4-dichlorophenol
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- 2,4-dichlorphenol was used as reference batch in the concentrations of 30, 70 and 100 mg/L. The EC50 was between 30 and 100 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is toxic to microorganisms at an EC50 of 2.3 mg/L and an EC10 of 0.1 mg/L.
- Executive summary:
The bacterial toxicity was investigated in a cell multiplication inhibition test with Pseudomonas putida in accordance with the guideline DIN 38 412, part 8. The test organism were exposed to concentration between 0.1 and 250 mg/L for 16 h. The effect concentration of the test substance was determined at 2.3 mg/L (EC50) and 0.1 mg/L (EC10).
Reference
Description of key information
The bacterial toxicity was investigated in a cell multiplication inhibition test with Pseudomonas putida in accordance with the guideline DIN 38 412, part 8. The test organism were exposed to concentration between 0.1 and 250 mg/L for 16 h. The effect concentration of the test substance was determined at 2.3 mg/L (EC50) and 0.1 mg/L (EC10).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2.3 mg/L
- EC10 or NOEC for microorganisms:
- 0.1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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