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EC number: 272-897-9 | CAS number: 68919-40-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 28, 2019 - April 18, 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Solid content: 99.7%.
- Carbon content: 60.15% (determined by Hydrotox with a total carbon analyzer) . - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: municipal wastewater treatment plant Breisgauer Bucht.
- Concentration of sludge: the dry solid content of the activated sludge was 4.2 g/L. It was determined by weight measurements after drying at 105°C for 4 hours (mean of triplicate measurements).
- Pretreatment: the activated sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- PRERATION OF THE SOLUTIONS:
Test item:
The test item was added directly into the test vessels. 49.2 – 50.6 mg of the test item were added into the test vessels corresponding to a concentration of 19.7 – 20.3 mg/L organic carbon.
Toxicity control:
49.5 mg of the test item and 5.15 mL of the reference stock solution (10 g/L) were added into the toxicity control vessel corresponding to a concentration of 39.8 mg/L organic carbon.
TEST CONDITIONS
- Composition of medium: Mineral medium as described in the OECD 301
- Test temperature: The temperature was 20.7 – 22.4°C throughout the whole study and therefore within the required range of 22 ± 2°C.
- pH adjusted: no
- Aeration: The aeration rate was kept at a rate of 30 - 100 mL / min (1.6 - 5.5 bubbles / second) and determined visually daily on working days. (counted bubbles: 2.5 – 5.3 bubbles/second).
- Suspended solids concentration: 30 mg/L dry solids.
- Continuous darkness: yes
TEST SYSTEM:
- Number of flasks: In total three reactors containing the test item with inoculum, three reactors containing only inoculum (blank), three reactors containing the reference compound with inoculum and one reactor containing the test item and reference compound with inoculum (toxicity control) were set up.
- Method used to create aerobic conditions: The CO2-free air production system consists of an air compressor, three 1000 mL gas wash bottles filled with dry soda lime in series followed by one bottle filled with 0.1 M NaOH (sodium hydroxide).
- Measuring equipment: The CO2 produced in the reactors was absorbed in two 250 mL gas wash bottles in series each
filled with 200 mL 0.2 M NaOH.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 reactors containing only inoculum (blank)
- Toxicity control: yes, one reactor containing the test item and reference compound with inoculum (toxicity control) were set up.
- Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 43.6
- Sampling time:
- 28 d
- Details on results:
- TEST ITEM:
The degradation extent of the test item at the end of the test was 43.6% of ThCO2 at 28 d after acidification (mean of three replicates). See 'Any other information on results incl. tables'.
TOXICITY CONTROL:
The degradation extent in the toxicity control was 56.9% within 14 days. According to the guideline, the test substance had no inhibitory effect on the inoculum. See 'Any other information on results incl. tables'. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation of the test item at the end of the test was 43.6% of ThCO2 (28 d after acidification, mean of three replicates). Therefore, the test item did not reach the criteria for ready biodegradability (60% of ThCO2 within a 10-d window). The degradation of the toxicity control after 14 days was 56.9%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.
- Executive summary:
The ready biodegradability of the test item was investigated in a GLP-compliant study performed in accordance with OECD Guideline 301B. The degradation of the toxicity control after 14 days was 56.9%. Thus, the test item had no inhibitory effect on the inoculum according to the criterion of the guideline. The degradation of the test item at the end of the test was 43.6% of ThCO2 (28 d after acidification, mean of three replicates). Therefore, the test item did not reach the criteria for ready biodegradability (60% of ThCO2 within a 10-d window).
Reference
Ultimate biodegradation after x days [% of ThCO2]
Days |
0 |
4 |
7 |
11 |
14 |
21 |
28 |
29 |
Test item flasks |
0 |
12.5 |
20.0 |
26.9 |
30.0 |
38.6 |
43.1 |
45.1 |
0 |
14.2 |
22.9 |
30.4 |
36.3 |
4.08 |
44.2 |
42.7 |
|
0 |
16.8 |
24.4 |
30.4 |
33.8 |
36.8 |
43.3 |
43.1 |
|
Reference flasks |
0 |
72.1 |
78.1 |
82.4 |
84.8 |
83.8 |
84.4 |
84.1 |
0 |
71.7 |
72.9 |
80.0 |
81.6 |
85.1 |
85.9 |
87.3 |
|
0 |
72.5 |
78.9 |
84.8 |
85.6 |
85.2 |
84.7 |
86.7 |
|
Toxicity control |
0 |
40.4 |
49.5 |
55.1 |
56.9 |
62.1 |
64.8 |
64.8 |
Validity criteria:
- The IC content in the test vessel was less than 5% of the TOC introduced with the test item.
- The CO2 evolution in the inoculum blank at the end of the test was below 40 mg/L.
- The difference of extremes of replicate values of the test item at the end of the test was less than 20%.
- The biodegradation of the reference compound reached the pass level of 60% ThCO2 by day 4.
- The degradation extent in the toxicity control was above 25% in 14 days based on ThCO2.
Description of key information
The ready biodegradability of the test item was investigated in a GLP-compliant study (Flach, 2018) performed in accordance with OECD Guideline 301B. The degradation of the toxicity control after 14 days was 56.9%. Thus, the test item had no inhibitory effect on the inoculum according to the criterion of the guideline. The degradation of the test item at the end of the test was 43.6% of ThCO2 (28 d after acidification, mean of three replicates). Therefore, the test item did not reach the criteria for ready biodegradability (60% of ThCO2 within a 10-d window).
Key value for chemical safety assessment
- Type of water:
- freshwater
Additional information
The aerobic biodegradability of the test item was investigated in GLP-compliant study (Flach, 2018) performed in accordance with standard methods, without deviations. The study is considered as reliable (Klimisch 1) and is selected as a key study for the endpoint.
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